FB2024_03 , released June 25, 2024
Allele: Dmel\obst-EUAS.a
Open Close
General Information
Symbol
Dmel\obst-EUAS.a
Species
D. melanogaster
Name
FlyBase ID
FBal0325125
Feature type
allele
Associated gene
Dmel\obst-E
Associated Insertion(s)
Carried in Construct
P{UAS-obst-E.a}
Key Links
Transgenic product class
cDNA
(Tajiri et al., 2017)
wild_type
(Tajiri et al., 2017)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Tajiri et al., 2017)
wild_type
(Tajiri et al., 2017)
Mutagen
in vitro construct
(Tajiri et al., 2017)
Progenitor genotype
Carried in construct
P{UAS-obst-E.a}
Cytology
Description

UASt regulatory sequences drive expression of cDNA (from GH01453) for splice variant a of obst-E.

(Tajiri et al., 2017)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
obst-E
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The body shape of pupae expressing obst-EScer\UAS.a under the control of Scer\GAL4Act5C.PU is not significantly different from controls and no significant abnormalities are observed in the morphology of first, second or third instar larval cuticle.

      (Tajiri et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4Act5C.PU/obst-EUAS.a rescues obst-EDel/obst-ECPTI002377

      (Tajiri et al., 2017)

      Scer\GAL4Act5C.PU, obst-EUAS.a, obst-EUAS.b rescues obst-EDel/obst-EKO

      (Tajiri et al., 2017)
      Partially rescues

      Scer\GAL4Act5C.PU/obst-EUAS.a partially rescues obst-EDel/obst-EKO

      (Tajiri et al., 2017)
      Comments

      The pupal shape defects (pupae are longer and thinner compared to wild-type and have higher axis ratio) characteristic for obst-ECPTI002377/obst-EDel transheterozygotes is rescued by expression of obst-EScer\UAS.a under the control of Scer\GAL4Act5C.PU in the mutant background. Expression of obst-EScer\UAS.a only weakly rescues the larval lethality of obst-EKO/obst-EDel transheterozygotes but the pupae of the escapers have body shape with an axis ratio only slightly (though significantly) increased compared to wild-type. Simultaneous expression of obst-EScer\UAS.b and obst-EScer\UAS.a rescues both the lethality and the pupal shape defects of obst-EKO/obst-EDel animals.

      (Tajiri et al., 2017)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      obst-EScer\UAS.a
      obst-EUAS.a
      Name Synonyms
      Secondary FlyBase IDs
        References (1)