Scer\GAL4Act5C.PU-driven expression of obst-EScer\UAS.b rescues the lethality of obst-EKO/obst-EDel transheterozygotes but the 'rescue' pupae show body shape defects (are thinner and longer than controls) due to failed shape change and contraction upon dehydration of the larval cuticle during metamorphosis. The prepupae show normal internalization of the anterior segments but the anterior spiracles fail to evert and remain encapsulated within the puparium. In contrast to wild-type, no ridges are observed on the inner surface of third instar larval cuticle of the mutant animals and the surface appears essentially flat with the exception of a narrow lateral region where some of the ridging is partially preserved. Although the general three-layer organization of the cuticle is maintained, unlike the lamellae in wild-type, the lamellae in the procuticle layer of the mutants are flat, of constant thickness and tend to contain unidirectional arcs. No apparent morphological aberration are observed in the cuticle of first or second instar larvae.
Scer\GAL4Act5C.PU/obst-EUAS.b partially rescues obst-EDel/obst-EKO
Scer\GAL4Act5C.PU/obst-EUAS.b fails to rescue obst-EDel/obst-ECPTI002377
The pupal shape defects (pupae are longer and thinner compared to wild-type) characteristic for obst-ECPTI002377/obst-EDel transheterozygotes is not rescued by expression of obst-EScer\UAS.b under the control of Scer\GAL4Act5C.PU in the mutant background. Similarly, Scer\GAL4Act5C.PU-driven expression of obst-EScer\UAS.b rescues the larval lethality of obst-EKO/obst-EDel transheterozygotes but the rescued pupae are abnormal in shape, despite having normal body shape during the larval stages. Simultaneous expression of obst-EScer\UAS.b and obst-EScer\UAS.a rescues both the lethality and the pupal shape defects of obst-EKO/obst-EDel animals.