FB2024_03 , released June 25, 2024
Allele: Dmel\lilliUAS.SR.Tag:FLAG
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General Information
Symbol
Dmel\lilliUAS.SR.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0327022
Feature type
allele
Associated gene
Dmel\lilli
Associated Insertion(s)
Carried in Construct
P{UAS-lilli.SR.FLAG}
Key Links
Transgenic product class
cDNA
(Liu et al., 2017)
wild_type
(Liu et al., 2017)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Liu et al., 2017)
wild_type
(Liu et al., 2017)
Mutagen
in vitro construct
(Liu et al., 2017)
Progenitor genotype
Carried in construct
P{UAS-lilli.SR.FLAG}
Cytology
Description

UASt regulatory sequences drive a shmiRNA resistant form (silent nucleotide mutations) of full-length lilli cDNA (DGRC AT02676 with the following corrected: a missense mutation (D1142I) and deletion of two base pairs (C3452 and C3453)), tagged with Tag:FLAG at the N-terminus.

(Liu et al., 2017)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
lilli
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of lilliScer\UAS.SR.T:Zzzz\FLAG under the control of Scer\GAL4pnt-14-94 does not lead a significant change in the number of type II neuroblasts per third instar larval brain lobe, as compared to controls.

      (Liu et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressed by
      Statement
      Reference

      Scer\GAL4pnt-14-94, Su(Tpl)UAS.SR.Tag:MYC, lilliUAS.SR.Tag:FLAG has increased cell number | third instar larval stage phenotype, suppressible | partially by Df(3R)grob32.2/+

      (Liu et al., 2017)

      Scer\GAL4insc-Mz1407, Su(Tpl)UAS.SR.Tag:MYC, lilliUAS.SR.Tag:FLAG has increased cell number | third instar larval stage phenotype, suppressible by Cdk9D199N.UAS.SR, Scer\GAL4insc-Mz1407

      (Liu et al., 2017)

      Scer\GAL4insc-Mz1407, Su(Tpl)UAS.SR.Tag:MYC, lilliUAS.SR.Tag:FLAG has increased cell number | third instar larval stage phenotype, suppressible by NGD144, Scer\GAL4insc-Mz1407

      (Liu et al., 2017)

      Scer\GAL4insc-Mz1407, Su(Tpl)UAS.SR.Tag:MYC, lilliUAS.SR.Tag:FLAG has increased cell number | third instar larval stage phenotype, suppressible by DeltaDN.UAS, Scer\GAL4insc-Mz1407

      (Liu et al., 2017)

      Scer\GAL4pnt-14-94, Su(Tpl)UAS.SR.Tag:MYC, lilliUAS.SR.Tag:FLAG has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by Df(3R)grob32.2/+

      (Liu et al., 2017)

      Scer\GAL4insc-Mz1407, Su(Tpl)UAS.SR.Tag:MYC, lilliUAS.SR.Tag:FLAG has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Cdk9D199N.UAS.SR, Scer\GAL4insc-Mz1407

      (Liu et al., 2017)

      Scer\GAL4insc-Mz1407, Su(Tpl)UAS.SR.Tag:MYC, lilliUAS.SR.Tag:FLAG has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by NGD144, Scer\GAL4insc-Mz1407

      (Liu et al., 2017)

      Scer\GAL4insc-Mz1407, Su(Tpl)UAS.SR.Tag:MYC, lilliUAS.SR.Tag:FLAG has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by DeltaDN.UAS, Scer\GAL4insc-Mz1407

      (Liu et al., 2017)
      Suppressor of
      Other
      Statement
      Reference

      Scer\GAL4pnt-14-94, Su(Tpl)UAS.SR.Tag:MYC, lilliUAS.SR.Tag:FLAG has abnormal neuroanatomy | third instar larval stage phenotype

      (Liu et al., 2017)

      Scer\GAL4pnt-14-94, Su(Tpl)UAS.SR.Tag:MYC, lilliUAS.SR.Tag:FLAG has increased occurrence of cell division | third instar larval stage phenotype

      (Liu et al., 2017)

      Scer\GAL4insc-Mz1407, Su(Tpl)UAS.SR.Tag:MYC, lilliUAS.SR.Tag:FLAG has abnormal neuroanatomy | third instar larval stage phenotype

      (Liu et al., 2017)

      Scer\GAL4insc-Mz1407, Su(Tpl)UAS.SR.Tag:MYC, lilliUAS.SR.Tag:FLAG has abnormal size | third instar larval stage phenotype

      (Liu et al., 2017)

      Scer\GAL4insc-Mz1407, Su(Tpl)UAS.SR.Tag:MYC, lilliUAS.SR.Tag:FLAG has increased cell number | third instar larval stage phenotype

      (Liu et al., 2017)

      Scer\GAL4pnt-14-94, Su(H)UAS.Tag:V5, Su(Tpl)UAS.SR.Tag:MYC, lilliUAS.SR.Tag:FLAG has abnormal neuroanatomy | third instar larval stage phenotype

      (Liu et al., 2017)

      Scer\GAL4pnt-14-94, Su(H)UAS.Tag:V5, Su(Tpl)UAS.SR.Tag:MYC, lilliUAS.SR.Tag:FLAG has neoplasia | third instar larval stage phenotype

      (Liu et al., 2017)

      Scer\GAL4pnt-14-94, Su(H)UAS.Tag:V5, Su(Tpl)UAS.SR.Tag:MYC, lilliUAS.SR.Tag:FLAG has increased occurrence of cell division | third instar larval stage phenotype

      (Liu et al., 2017)

      Scer\GAL4pnt-14-94, Su(Tpl)UAS.SR.Tag:MYC, lilliUAS.SR.Tag:FLAG has increased cell number | somatic clone | third instar larval stage phenotype

      (Liu et al., 2017)

      Scer\GAL4pnt-14-94, Su(Tpl)UAS.SR.Tag:MYC, lilliUAS.SR.Tag:FLAG has abnormal size | third instar larval stage phenotype

      (Liu et al., 2017)

      Scer\GAL4pnt-14-94, Su(Tpl)UAS.SR.Tag:MYC, lilliUAS.SR.Tag:FLAG has abnormal neuroanatomy | somatic clone | third instar larval stage phenotype

      (Liu et al., 2017)

      Scer\GAL4pnt-14-94, Su(Tpl)UAS.SR.Tag:MYC, lilliUAS.SR.Tag:FLAG has increased cell number | third instar larval stage phenotype

      (Liu et al., 2017)

      Scer\GAL4pnt-14-94, Su(Tpl)UAS.SR.Tag:MYC, lilliUAS.SR.Tag:FLAG has neoplasia | third instar larval stage phenotype

      (Liu et al., 2017)
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      Statement
      Reference

      Scer\GAL4insc-Mz1407, Su(Tpl)UAS.SR.Tag:MYC, lilliUAS.SR.Tag:FLAG has type II neuroblast | third instar larval stage phenotype, suppressible by NGD144, Scer\GAL4insc-Mz1407

      (Liu et al., 2017)

      Scer\GAL4insc-Mz1407, Su(Tpl)UAS.SR.Tag:MYC, lilliUAS.SR.Tag:FLAG has type II neuroblast | third instar larval stage phenotype, suppressible by DeltaDN.UAS, Scer\GAL4insc-Mz1407

      (Liu et al., 2017)

      Scer\GAL4pnt-14-94, Su(Tpl)UAS.SR.Tag:MYC, lilliUAS.SR.Tag:FLAG has embryonic/larval brain | third instar larval stage phenotype, suppressible | partially by Df(3R)grob32.2/+

      (Liu et al., 2017)

      Scer\GAL4insc-Mz1407, Su(Tpl)UAS.SR.Tag:MYC, lilliUAS.SR.Tag:FLAG has embryonic/larval brain | third instar larval stage phenotype, suppressible by Cdk9D199N.UAS.SR, Scer\GAL4insc-Mz1407

      (Liu et al., 2017)

      Scer\GAL4insc-Mz1407, Su(Tpl)UAS.SR.Tag:MYC, lilliUAS.SR.Tag:FLAG has embryonic/larval brain | third instar larval stage phenotype, suppressible by NGD144, Scer\GAL4insc-Mz1407

      (Liu et al., 2017)

      Scer\GAL4insc-Mz1407, Su(Tpl)UAS.SR.Tag:MYC, lilliUAS.SR.Tag:FLAG has embryonic/larval brain | third instar larval stage phenotype, suppressible by DeltaDN.UAS, Scer\GAL4insc-Mz1407

      (Liu et al., 2017)

      Scer\GAL4pnt-14-94, Su(Tpl)UAS.SR.Tag:MYC, lilliUAS.SR.Tag:FLAG has type II neuroblast | third instar larval stage phenotype, suppressible | partially by Df(3R)grob32.2/+

      (Liu et al., 2017)

      Scer\GAL4insc-Mz1407, Su(Tpl)UAS.SR.Tag:MYC, lilliUAS.SR.Tag:FLAG has type II neuroblast | third instar larval stage phenotype, suppressible by Cdk9D199N.UAS.SR, Scer\GAL4insc-Mz1407

      (Liu et al., 2017)
      Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The co-expression of lilliScer\UAS.SR.T:Zzzz\FLAG and Su(Tpl)Scer\UAS.SR.T:Hsap\MYC in third instar larval brains leads to a significant increase in the number of type II neuroblasts as compared to controls under the following conditions: constitutive or clonal co-expression under the control of Scer\GAL4pnt-14-94; constitutive co-expression at high levels, but not at low levels, under the control of Scer\GAL4insc-Mz1407. The neuroblasts derived from Scer\GAL4pnt-14-94-driven co-expression display normal cortical polarity but are highly proliferative, giving rise to an enlargement of the brain, and their transplantation into the abdomen of wild-type adults leads to highly proliferative overgrowths that metastasize to distant tissues, as compared to controls.

      The increased number of type II neuroblasts resulting from Scer\GAL4pnt-14-94-driven co-expression of lilliScer\UAS.SR.T:Zzzz\FLAG and Su(Tpl)Scer\UAS.SR.T:Hsap\MYC is enhanced by one copy of E(spl)mγ-HLHT:Avic\GFP and partially suppressed by one copy of Df(3R)grob32.2; the increased number of type II neuroblast resulting from Scer\GAL4insc-Mz1407-driven co-expression is enhanced by the additional co-expression of Su(H)Scer\UAS.T:SV5\V5, is fully suppressed by the additional co-expression of Cdk9D199N.Scer\UAS.SR and is even converted into a near absence of type II neuroblasts as compared to controls by the additional co-expression of either NGD144 (together with Dicer-2 transgene, for efficient RNAi) or DlD.Scer\UAS.

      The co-expression of lilliScer\UAS.SR.T:Zzzz\FLAG and Su(Tpl)Scer\UAS.SR.T:Hsap\MYC under the control of Scer\GAL4ase.neuro does not lead to significant changes in the number of type I neuroblasts in the third instar larval brain, as compared to controls.

      The combined co-expression at low levels of lilliScer\UAS.SR.T:Zzzz\FLAG and Su(Tpl)Scer\UAS.SR.T:Hsap\MYC fully suppresses the decreased number of type II neuroblasts in third instar larval brain lobes resulting from the expression of aPKCHMS01320 under the control of Scer\GAL4insc-Mz1407.

      (Liu et al., 2017)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      lilliScer\UAS.SR.T:Zzzz\FLAG
      lilliUAS.SR.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (1)