FlyBase Allele Report: Crei\ChR1::Vcar\ChR1::Vcar\ChR2[ReaChR.UAS.Citrine]

FB2024_03 , released June 25, 2024

FB2024_03 , released June 25, 2024

Allele: Crei\ChR1::VcarChR1::VcarChR2^{ReaChR.UAS.Citrine}

General Information

Symbol

Crei\ChR1::Vcar\ChR1::Vcar\ChR2^{ReaChR.UAS.Citrine}

Species

C. reinhardtii

Name

FlyBase ID

FBal0351553

Feature type

allele

Associated gene

Crei\ChR1::Vcar\ChR1::Vcar\ChR2

Associated Insertion(s)

Carried in Construct

PBac{UAS-ReaChR}

Also Known As

UAS-ReaChR, UAS-ReaChR::Citrine

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Inagaki and Anderson, 2013.11.13)

Progenitor genotype

Carried in construct

PBac{UAS-ReaChR}

Cytology

Description

UAS regulatory sequences drive expression of Citrine-tagged ReaChR.

(Inagaki and Anderson, 2013.11.13)

Allele components

Component

Use(s)

Regulatory region(s)

binary expression system - regulatory region

Encoded product / tool

light-regulated neuron activation tool

Tagged with

yellow fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | adult stage | conditional, with Hsap\RELA^AD.R15A01, Scer\GAL4^DBD.R71G01

(Hoopfer et al., 2015)

abnormal behavior | adult stage | conditional, with Scer\GAL4^GMR21D02

(Owald et al., 2015)

abnormal courtship behavior | conditional, with Hsap\RELA^AD.R15A01, Scer\GAL4^DBD.R71G01

(Hoopfer et al., 2015)

abnormal feeding behavior | adult stage | conditional, with Ecol\lexA^GMR65C11, Scer\GAL4^Gr28b.b.4.7, Scer\GAL80^{IVS.WPRE.8xlexAop}

(Zhan et al., 2016)

abnormal feeding behavior | adult stage | conditional, with Scer\GAL4^AstA.2.1

(Zhan et al., 2016)

abnormal feeding behavior | adult stage | conditional, with Scer\GAL4^AstA.2.1, Scer\GAL4^Gr28b.b.4.7

(Zhan et al., 2016)

abnormal feeding behavior | adult stage | conditional, with Scer\GAL4^Dh44.PU, Scer\GAL4^Gr28b.b.4.7

(Zhan et al., 2016)

abnormal feeding behavior | adult stage | conditional, with Scer\GAL4^Gr28b.b.4.7

(Zhan et al., 2016)

abnormal feeding behavior | adult stage | conditional, with Scer\GAL4^Gr28b.b.4.7, Scer\GAL4^Gr43a-GAL4

(Zhan et al., 2016)

abnormal feeding behavior | adult stage | conditional, with Scer\GAL4^Gr28b.b.4.7, Scer\GAL4^Ilp2.PU

(Zhan et al., 2016)

abnormal locomotor behavior | adult stage | conditional, with Hsap\RELA^AD.R15A01, Scer\GAL4^DBD.R71G01

(Hoopfer et al., 2015)

abnormal locomotor behavior | adult stage | conditional, with Scer\GAL4^GMR38E07

(Hu et al., 2018)

abnormal locomotor behavior | adult stage | conditional, with Scer\GAL4^GMR49H02

(Hu et al., 2018)

abnormal locomotor behavior | adult stage | conditional, with Scer\GAL4^GMR89E07

(Hu et al., 2018)

abnormal locomotor behavior | adult stage | conditional, with Scer\GAL4^RapGAP1-OK6

(Dawydow et al., 2014)

abnormal locomotor behavior | larval stage | conditional, with Scer\GAL4^RapGAP1-OK6

(Dawydow et al., 2014)

abnormal neurophysiology | adult stage | conditional, with Scer\GAL4^GH146

(Jeanne et al., 2018)

abnormal pain response | larval stage | conditional, with Scer\GAL4^GMR52F05

(Yoshino et al., 2017)

abnormal pain response | larval stage | conditional, with Scer\GAL4^GMR94B10

(Yoshino et al., 2017)

paralytic | adult stage | conditional, with Scer\GAL4^RapGAP1-OK6

(Dawydow et al., 2014)

paralytic | larval stage | conditional, with Scer\GAL4^RapGAP1-OK6

(Dawydow et al., 2014)

Phenotype Manifest In

adult lateral horn neuron | conditional, with Scer\GAL4^GH146

(Jeanne et al., 2018)

Kenyon cell | adult stage | conditional, with Scer\GAL4^GH146

(Jeanne et al., 2018)

Detailed Description

Statement

Reference

When retinal is not added to the food, larvae expressing Crei\ChR1^{ReaChR.Scer\UAS.T:Crei\ChR2,T:Avic\GFP-mCitrine} display a relatively small reaction to photostimulation, as measured by the degree of immobilization. When retinal is added, light application induces reversible muscle paralysis, with the duration of immobilization scaling with light intensity.

Mid-intensity green or blue light immobilizes adult flies expressing Crei\ChR1^{ReaChR.Scer\UAS.T:Crei\ChR2,T:Avic\GFP-mCitrine} via Scer\GAL4^RapGAP1-OK6, dependent on retinal supplementation.

(Dawydow et al., 2014)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Crei\ChR1::Vcar\ChR1::Vcar\ChR2^{ReaChR.UAS.Citrine}, Scer\GAL4^Gr28b.b.4.7 has abnormal feeding behavior | adult stage | conditional phenotype, suppressible | partially by amon^RNAi.UAS.cRa, Scer\GAL4^Gr28b.b.4.7

(Zhan et al., 2016)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

53741

w^*; P{UAS-ReaChR}attP40

53748

w^*; P{UAS-ReaChR}su(Hw)attP5/CyO

53742

w^*; P{UAS-ReaChR}attP40/CyO; PBac{UAS-GCaMP3.T}VK00005

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Crei\ChR1::Vcar\ChR1::Vcar\ChR2^{ReaChR.UAS.Citrine}

Crei\ChR1^{ReaChR.Scer\UAS.T:Crei\ChR2,T:Avic\GFP-mCitrine}

Crei\ChR1^{ReaChR.UAS.Citrine}

Name Synonyms

Secondary FlyBase IDs

FBal0291763
FBal0351222

References (30)

Report Sections