dSEPT7, septin
member of the septin family of polymerizing GTPases - functions together with Septin 1 and Septin 2 in organizing the cytoskeleton during cytokinesis and septum formation
Please see the JBrowse view of Dmel\pnut for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.49
2.5 (northern blot)
539 (aa); 60 (kD observed); 60 (kD predicted)
Likely part of a multicomponent septin complex that includes Septin1 (PubMed:8590810). Interacts with Septin1 (PubMed:8590810). Interacts with hil (PubMed:15818553). Interacts with park (PubMed:17456438).
Ubiquitinated by park, leading to its degradation by the proteasome.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\pnut using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
pnut protein is detected on the cell surfaces of the embryonic CNS and on the apical membranes of developing photoreceptor cells in the eye imaginal disc.
JBrowse - Visual display of RNA-Seq signals
View Dmel\pnut in JBrowsePlease Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
monoclonal
New stable cell line derived from S2-unspecified : A stable S2 cell line expressing pnut tagged with Tag:FLAG was generated.
dsRNA made from templates generated with primers directed against pnut that is transfected into S2 treated with Listeria monocytogenes reveals pnut to be involved in Listeria monocytogenes intracellular growth, with less intracellular growth observed in pnut-treated cells. pnut also seems to be involved in L.monocytogenes entry.
dsRNA directed against this gene causes defects in cytokinesis when tested in an RNAi screen in S2 cells.
A complex of septin polypeptides is isolated that bind and hydrolyse GTP and form filaments. Their high degree of conservation, ubiquitous expression and proven role in cytokinesis suggests septins are certain to be important players in regulating cell architecture and function. Septins alone can form regular filamentous polymers, since filament formation is likely to be central to their localisation and function.
