FlyBase Gene Report: Dmel\DCP2

FB2024_04 , released June 25, 2024

FB2024_04 , released June 25, 2024

Gene: Dmel\DCP2

General Information

Symbol

Dmel\DCP2

Species

D. melanogaster

Name

Decapping protein 2

Annotation Symbol

CG6169

Feature Type

protein_coding_gene

FlyBase ID

FBgn0036534

Gene Model Status

Stock Availability

8 publicly available

Enzyme Name (EC)

5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase (3.6.1.62)

Gene Summary

Decapping protein 2 (DCP2) encodes a m7G(5')pppN diphosphatase involved in gene silencing by miRNA and the regulation of cytoplasmic mRNA processing body assembly. [Date last reviewed: 2019-09-12] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

dDcp2, l(3)tb

Key Links

Genomic Location

Cytogenetic map

Sequence location

3L:15,818,734..15,826,423 [-]

Recombination map

3-43

RefSeq locus

NT_037436 REGION:15818734..15826423

Sequence

Get Decorated FASTA

Genomic Maps

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (16 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

enables 5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase activity

inferred from direct assay

(Lin et al., 2008)

enables actin filament binding

inferred from direct assay

(Lee et al., 2020)

enables protein binding

inferred from physical interaction with UniProtKB:Q9VKK1

(Jinek et al., 2008)

inferred from physical interaction with UniProtKB:Q9VVI2

(Tritschler et al., 2007)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables 5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase activity

inferred from electronic annotation with InterPro:IPR044099

(InterPro Project Members, 2004-)

enables manganese ion binding

inferred from electronic annotation with InterPro:IPR036189

(InterPro Project Members, 2004-)

enables RNA binding

inferred from electronic annotation with InterPro:IPR007722, InterPro:IPR036189

(InterPro Project Members, 2004-)

Biological Process (8 terms)

Terms Based on Experimental Evidence (8 terms)

CV Term

Evidence

References

involved_in cortical actin cytoskeleton organization

inferred from mutant phenotype

(Lee et al., 2020)

involved_in deadenylation-dependent decapping of nuclear-transcribed mRNA

inferred from direct assay

(Lin et al., 2008)

involved_in deadenylation-independent decapping of nuclear-transcribed mRNA

inferred from mutant phenotype

(Arvola et al., 2020)

involved_in miRNA-mediated post-transcriptional gene silencing

inferred from mutant phenotype

(Rehwinkel et al., 2005)

involved_in nuclear-transcribed mRNA catabolic process

inferred from mutant phenotype

(Bönisch et al., 2007)

involved_in pole plasm oskar mRNA localization

inferred from mutant phenotype

(Lee et al., 2020)

involved_in regulation of cytoplasmic mRNA processing body assembly

inferred from mutant phenotype

(Lin et al., 2008)

NOT involved_in nuclear-transcribed mRNA catabolic process, nonsense-mediated decay

inferred from mutant phenotype

(Rehwinkel et al., 2005)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in deadenylation-dependent decapping of nuclear-transcribed mRNA

inferred from electronic annotation with InterPro:IPR044099

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000561807

(GO Reference Genome Project, 2011-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Lin et al., 2008)

located_in P-body

inferred from direct assay

(Lee et al., 2020, Eulalio et al., 2007)

located_in pole plasm

inferred from direct assay

(Lee et al., 2020)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000561806

(GO Reference Genome Project, 2011-)

is_active_in P-body

inferred from biological aspect of ancestor with PANTHER:PTN000561808

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

RNA DECAPPING ENZYMES

Protein Family (UniProt)

-

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase activity

a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + H2O = a 5'-end phospho-ribonucleoside in mRNA + 2 H(+) + N(7)-methyl-GDP (3.6.1.62)

RHEA 67484:

Summaries

Gene Snapshot

Decapping protein 2 (DCP2) encodes a m7G(5')pppN diphosphatase involved in gene silencing by miRNA and the regulation of cytoplasmic mRNA processing body assembly. [Date last reviewed: 2019-09-12]

Gene Group (FlyBase)

RNA DECAPPING ENZYMES -: RNA decapping enzymes catalyze the removal/degradation of the 5' cap structure that is present on most eukaryotic mRNAs and small nuclear RNAs, and thereby modulate RNA turnover. Canonical decapping enzymes (Nudix hydrolases, such as DCP2) hydrolyse a pyrophosphate bond in the 5' N7-methyl guanosine (m7G) cap of intact RNA molecules, thereby exposing a free 5' monophosphate to nucleases and initiating a 5' -> 3' decay pathway. Others (HIT family proteins, such as DCPS) act as scavengers and cleave a pyrophosphate bond of m7G capped di- or oligonucleotide remnants of the 3' -> 5' decay pathway. Members of the diverse DXO protein family (e.g. Rai1/DXO) are mainly involved in mRNA cap quality control and selectively cleave bonds in the 5' end of uncapped (triphosphorylated) mRNAs or mRNAs with an unmethylated cap. DXO family proteins are also involved in the removal of the NAD+ cap variant. (Adapted from PMID:30345629.)

Gene Model and Products

Number of Transcripts

4

Number of Unique Polypeptides

3

Please see the JBrowse view of Dmel\DCP2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q5U127)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.45

Sequence Ontology: Class of Gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0075538

3665

791

FBtr0075539

2850

564

FBtr0100528

2690

564

FBtr0304975

5934

792

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

FBpp0075293

87.5

791

10.11

FBpp0075294

63.3

564

10.10

FBpp0099969

63.3

564

10.10

FBpp0293514

87.6

792

10.11

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

564 aa isoforms: DCP2-PB, DCP2-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\DCP2 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.85

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

organism | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

(Lin et al., 2008)

oogenesis stage S1 -- S9

• cytoplasm

(Lin et al., 2006)

oogenesis stage S6

(Lee et al., 2020)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

(Lee et al., 2020)

first instar larval stage

(Lee et al., 2020)

adult stage | female

(Lee et al., 2020)

Additional Descriptive Data

DCP2-PA is the major isoform expressed in ovaries, embryos, and first instar larvae. DCP2 colocalizes with DCP1 and Ge-1 in the cytoplasm of oogenesis stage 6 nurse cells. It localizes with Ge-1 to the osk mRNP at the oocyte posterior cortex at stage S10.

(Lee et al., 2020)

DCP2 protein is observed in the cytoplasm of nurse cells from early oogeneis stages to stage 8-9. At stage 10, cytoplasmic foci in nurse cells are greatly reduced and staining is concentrated mainly in the nucleus.

(Lin et al., 2006)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Lin et al., 2008)

located_in P-body

inferred from direct assay

(Lee et al., 2020, Eulalio et al., 2007)

located_in pole plasm

inferred from direct assay

(Lee et al., 2020)

Expression Deduced from Reporters

Reporter: P{GT1}DCP2^BG01766

Stage

Tissue/Position (including subcellular localization)

Reference

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\DCP2 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\DCP2

View exonic expression by tissue for Dmel\DCP2

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0036534

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39722/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0036534

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0036534

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0036534

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6169

Flygut - An atlas of the Drosophila adult midgut

FBgn0036534

Images

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 9 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 14 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of DCP2

Transgenic constructs containing regulatory region of DCP2

Aberrations (Deficiencies and Duplications) ( 12 )

Inferred from experimentation ( 12 )

Gene disrupted in

Gene partially disrupted in

Df(3L)dbo^henji-1

(Wang et al., 2016)

Df(3L)dbo^henji-8

(Wang et al., 2016)

Gene not disrupted in

(Mishra et al., 2020)

(Mishra et al., 2020)

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^29BD

DCP2^GS3219

lethal, with Scer\GAL4^c355

DCP2^GS3219

lethal, with Scer\GAL4^dpp.blk1

DCP2^GS3219

lethal, with Scer\GAL4^hs.2sev

DCP2^GS3219

lethal | recessive

DCP2^e00034

lethal - all die before end of first instar larval stage

DCP2^del21

lethal - all die before end of P-stage | recessive

DCP2^tb

lethal - all die before end of pupal stage

DCP2^BG01766

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

DCP2^GD11844

lethal - all die before end of pupal stage | recessive

DCP2^tb

lethal - all die before end of second instar larval stage

DCP2^GS3219

lethal - all die before end of second instar larval stage (with DCP2^BG01766)

DCP2^GS3219

lethal - all die before end of second instar larval stage (with DCP2^GS3219)

DCP2^BG01766

lethal - all die during P-stage (with DCP2^BG01766)

DCP2^e00034

lethal - all die during P-stage (with DCP2^e00034)

lethal - all die during P-stage (with DCP2^tb)

DCP2^e00034

partially lethal - majority die (with DCP2^BG01766)

DCP2^tb

partially lethal - majority die (with DCP2^tb)

DCP2^BG01766

DCP2^c02419

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

DCP2^GD11844

viable, with Scer\GAL4^tin.CΔ4

DCP2^GD11844

Sterility

Allele

female semi-sterile (with DCP2^BG01766)

DCP2^tb

female semi-sterile (with DCP2^tb)

DCP2^BG01766

male semi-sterile (with DCP2^BG01766)

DCP2^tb

male semi-sterile (with DCP2^tb)

DCP2^BG01766

Other Phenotypes

Allele

abnormal developmental rate (with DCP2^BG01766)

abnormal developmental rate (with DCP2^e00034)

abnormal developmental rate (with DCP2^tb)

abnormal developmental rate | recessive

DCP2^tb

abnormal eclosion (with DCP2^BG01766)

DCP2^tb

abnormal eclosion (with DCP2^tb)

DCP2^BG01766

abnormal locomotor behavior, with Scer\GAL4^elav.PU

abnormal size | third instar larval stage

DCP2^tb

chemical resistant, with Scer\GAL4^elav.PU

chemical resistant | adult stage (with DCP2^c02419)

DCP2^e00034

chemical resistant | adult stage (with DCP2^e00034)

DCP2^c02419

chemical resistant | adult stage | recessive

DCP2^c02419

decreased cell size | larval stage

DCP2^tb

increased body size | P-stage

DCP2^tb

increased cell number | larval stage

DCP2^tb

increased cell number | larval stage (with DCP2^e00034)

DCP2^tb

increased cell number | larval stage (with DCP2^tb)

DCP2^e00034

increased occurrence of cell division | larval stage (with DCP2^e00034)

DCP2^tb

increased occurrence of cell division | larval stage (with DCP2^tb)

DCP2^e00034

increased occurrence of cell division | third instar larval stage

DCP2^tb

majority die during embryonic stage

DCP2^del21

majority die during P-stage (with DCP2^BG01766)

DCP2^tb

majority die during P-stage (with DCP2^tb)

DCP2^BG01766

majority die during P-stage | recessive

DCP2^tb

melanotic mass phenotype (with DCP2^BG01766)

DCP2^tb

melanotic mass phenotype (with DCP2^tb)

DCP2^BG01766

some die during pupal stage, with Scer\GAL4^pnr-MD237

DCP2^GD11844

some die during pupal stage | recessive

DCP2^tb

some die during third instar larval stage | recessive

DCP2^tb

Phenotype manifest in

Allele

abdominal ventral denticle belt | absent | embryonic stage | germline clone | maternal effect

DCP2^del21

adult abdomen (with DCP2^BG01766)

DCP2^tb

adult abdomen (with DCP2^tb)

DCP2^BG01766

adult optic lobe

DCP2^tb

adult thorax (with DCP2^BG01766)

DCP2^tb

adult thorax (with DCP2^tb)

DCP2^BG01766

embryonic/larval brain | larval stage

DCP2^tb

embryonic/larval brain | larval stage (with DCP2^BG01766)

DCP2^tb

embryonic/larval brain | larval stage (with DCP2^e00034)

DCP2^tb

embryonic/larval brain | larval stage (with DCP2^tb)

embryonic/larval optic lobe | larval stage

DCP2^tb

eye disc | third instar larval stage

DCP2^tb

filamentous actin | ectopic | oogenesis, with Scer\GAL4^{VP16.nanos.UTR}

DCP2^{UASp.PA.bcd3'UTR}

filamentous actin | germline clone

DCP2^del21

interommatidial bristle | absent (with DCP2^BG01766)

DCP2^tb

interommatidial bristle | absent (with DCP2^tb)

DCP2^BG01766

larval neuroblast | increased number

DCP2^tb

larval neuroblast | increased number (with DCP2^e00034)

DCP2^tb

larval neuroblast | increased number (with DCP2^tb)

DCP2^e00034

leg (with DCP2^BG01766)

DCP2^tb

leg (with DCP2^tb)

DCP2^BG01766

leg disc | third instar larval stage

DCP2^tb

male genitalia (with DCP2^BG01766)

DCP2^tb

male genitalia (with DCP2^tb)

DCP2^BG01766

microtubule | germline clone

DCP2^del21

ommatidium (with DCP2^BG01766)

DCP2^tb

ommatidium (with DCP2^tb)

DCP2^BG01766

oocyte, with Scer\GAL4^{VP16.nanos.UTR}

DCP2^{UASp.PA.bcd3'UTR}

oocyte | germline clone

DCP2^del21

P-body | germline clone

DCP2^del21

wing (with DCP2^BG01766)

DCP2^tb

wing (with DCP2^tb)

DCP2^BG01766

DCP2^tb

wing disc | larval stage

DCP2^tb

wing disc | larval stage (with DCP2^BG01766)

DCP2^tb

wing disc | larval stage (with DCP2^e00034)

DCP2^tb

wing disc | larval stage (with DCP2^tb)

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (19)

9 of 14

Yes

Yes

1

2 of 14

No

Yes

2 of 14

No

No

2 of 14

No

No

1

2 of 14

No

No

2 of 14

No

Yes

2 of 14

No

No

2 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1

1 of 14

No

No

1 of 14

No

No

1 of 14

No

Yes

1 of 14

No

Yes

1 of 14

No

No

1

1 of 14

No

Yes

1 of 14

No

Yes

1 of 14

No

No

1 of 14

No

No

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (17)

8 of 14

Yes

Yes

2 of 14

No

Yes

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

Yes

1 of 14

No

Yes

1 of 14

No

No

1 of 14

No

Yes

1 of 14

No

Yes

1 of 14

No

No

Mus musculus (laboratory mouse) (18)

9 of 14

Yes

Yes

2 of 14

No

Yes

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

Yes

1 of 14

No

Yes

1 of 14

No

No

1 of 14

No

Yes

1 of 14

No

Yes

1 of 14

No

No

1 of 14

No

No

Xenopus tropicalis (Western clawed frog) (9)

4 of 13

Yes

Yes

Xtro\LOC100495877

1 of 13

No

Yes

1 of 13

No

No

1 of 13

No

Yes

1 of 13

No

No

1 of 13

No

No

1 of 13

No

Yes

1 of 13

No

Yes

1 of 13

No

Yes

Danio rerio (Zebrafish) (18)

8 of 14

Yes

Yes

2 of 14

No

Yes

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

Yes

1 of 14

No

Yes

1 of 14

No

No

1 of 14

No

Yes

1 of 14

No

Yes

1 of 14

No

No

1 of 14

No

No

Caenorhabditis elegans (Nematode, roundworm) (9)

8 of 14

Yes

Yes

2 of 14

No

No

Cele\Y92H12BL.5

2 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

Yes

Anopheles gambiae (African malaria mosquito) (11)

Agam\AgaP_AGAP011114

9 of 12

Yes

Yes

Agam\AgaP_AGAP008495

2 of 12

No

No

Agam\AgaP_AGAP009901

2 of 12

No

No

Agam\AgaP_AGAP002403

1 of 12

No

No

Agam\AgaP_AGAP004158

1 of 12

No

No

Agam\AgaP_AGAP007969

1 of 12

No

No

Agam\AgaP_AGAP009926

1 of 12

No

No

Agam\AgaP_AGAP009927

1 of 12

No

No

Agam\AgaP_AGAP012590

1 of 12

No

No

Agam\AgaP_AGAP012737

1 of 12

No

No

Agam\AgaP_AGAP012857

1 of 12

No

No

Arabidopsis thaliana (thale-cress) (26)

9 of 13

Yes

Yes

2 of 13

No

No

2 of 13

No

No

2 of 13

No

No

2 of 13

No

No

2 of 13

No

No

2 of 13

No

No

2 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

Yes

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

Yes

1 of 13

No

Yes

1 of 13

No

No

1 of 13

No

Yes

1 of 13

No

No

1 of 13

No

No

Saccharomyces cerevisiae (Brewer's yeast) (6)

8 of 13

Yes

Yes

2 of 13

No

No

1 of 13

No

Yes

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

Schizosaccharomyces pombe (Fission yeast) (7)

7 of 12

Yes

Yes

2 of 12

No

No

1 of 12

No

No

Spom\SPAC14C4.10c

1 of 12

No

No

Spom\SPBC1778.03c

1 of 12

No

Yes

Spom\SPBP35G2.12

1 of 12

No

No

1 of 12

No

No

Escherichia coli (enterobacterium) (11)

3 of 11

Yes

Yes

2 of 11

No

No

1 of 11

No

No

1 of 11

No

No

1 of 11

No

Yes

1 of 11

No

No

1 of 11

No

No

1 of 11

No

No

1 of 11

No

No

1 of 11

No

No

1 of 11

No

No

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:DCP2. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (11)

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

DCP2; decapping mRNA 2

9 of 14

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

On

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

anti tag coimmunoprecipitation, anti tag western blot

(Braun et al., 2012)

anti tag coimmunoprecipitation, anti tag western blot

(Tritschler et al., 2008, Tritschler et al., 2007)

anti tag coimmunoprecipitation, anti tag western blot, western blot

(Lee et al., 2020, Jinek et al., 2008)

anti tag coimmunoprecipitation, anti tag western blot

(Haas et al., 2010)

anti tag coimmunoprecipitation, anti tag western blot

(Raisch et al., 2016)

Summary of Genetic Interactions

esyN Network Diagram

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

esyN Network Key:

Suppression

Enhancement

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Lee et al., 2020)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Gehrke et al., 2015)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0036534

MIST (protein-protein) - An integrated Molecular Interaction Database

39722

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

RNA degradation

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

mRNA decay by 5' to 3' exoribonuclease

Genomic Location and Detailed Mapping Data

Chromosome (arm)

3L

Recombination map

3-43

Cytogenetic map

Sequence location

3L:15,818,734..15,826,423 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

72A1-72A1

Limits computationally determined from genome sequence between P{EP}Tfb2^EP572 and P{lacW}th^j5C8&P{PZ}Mbs⁰³⁸⁰²

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-43

(FlyBase, 2016)

3-37.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (8)

Aberrations including deletions of this gene Aberrations including duplications of this gene

63369

w¹¹¹⁸; PBac{IT.GAL4}DCP2^0509-G4

85518

w¹¹¹⁸; PBac{RB}DCP2^e00034/TM6B, Tb¹

12794

w¹¹¹⁸; P{GT1}DCP2^BG01766/TM3, Sb¹ Ser¹

34806

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00115}attP2

200560

y¹ w^67c23; P{GSV1}DCP2^GS3219/TM3, Sb¹ Ser¹

HMS00115

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00115}attP2

v105130

P{KK101790}VIE-260B

v22272

w¹¹¹⁸ P{GD11844}v22272

Genomic Clones (15)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (37)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0036534

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0036534

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Lee et al., 2020)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene is transfected into S2 cells to study the genes role in decapping.

(Behm-Ansmant et al., 2006)

dsRNA has been made from templates generated with primers directed against this gene.

(Rehwinkel et al., 2005)

In contrast to the decapping enzymes in yeast and humans, the Dcp2 product does not seem to participate in the nonsense-mediated decay pathway.

(Rehwinkel et al., 2005)

Area matching Drosophila ESTs AI107445 and AA390813.

(Davies, 2001.3.15, Davies, 2001.3.15)

Relationship to Other Genes

Source for database merge of

Source for merge of: CG6169 BEST:LD07107

(Rana, 2004.7.8)

Source for merge of: DCP2 l(3)tb

(Mishra et al., 2020)

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Dcp2 CG6169

(Rehwinkel et al., 2005)

Source for identity of: DCP2 Dcp2

(FlyBase, 2009-)

Nomenclature comments

Symbol changed from 'Dcp2' to 'DCP2' to match the related 'DCP1' gene.

(FlyBase, 2009-)

Etymology

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

BEST:LD07107

CG6169

(Lee et al., 2020, Na et al., 2016, Wang et al., 2016, Schertel et al., 2013, Ren et al., 2012, Ni et al., 2011.7.19, Jäger and Dorner, 2010, Keleman et al., 2009.8.5, Zhou et al., 2008, Beckstead et al., 2007, Maines et al., 2007, Lin et al., 2006, Ma et al., 2006)

DCp2p

(Valencia-Sanchez et al., 2006)

Dcp2

(Benoit et al., 2022, Rosenthal and Yuan, 2021, Arvola et al., 2020, Nakajima et al., 2020, Foley et al., 2019, Swevers et al., 2018, Brewer-Jensen et al., 2016, Chen et al., 2014, Kim and Choe, 2014, Bonke et al., 2013, Pressman et al., 2012, Ren et al., 2012, Tan et al., 2012, Lin et al., 2008)

LD07107

(Toba et al., 1999)

dDCP2

(Lim et al., 2009)

dDcp

(Lin et al., 2008)

dDcp2

(Lee et al., 2020, Lin et al., 2008, Lin et al., 2008, Lin et al., 2006, Seydoux and Braun, 2006)

dcp2

(Pradhan et al., 2012)

l(3)tb

(Mishra et al., 2020, Roy et al., 1998)

Name Synonyms

CG6169

Decapping protein 2

(Chen et al., 2014, Tan et al., 2012, Lin et al., 2008)

lethal (3) tumorous brain

(Roy et al., 1998)

Secondary FlyBase IDs

FBgn0026583
FBgn0025745

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 67 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

39722

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q5U127

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q5U127

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0036534

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39722/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0036534

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0036534

FlyMine - An integrated database for Drosophila genomics

FBgn0036534

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG6169

MARRVEL_MODEL - MARRVEL (model organism gene)

39722

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0036534

DroID - A comprehensive database of gene and protein interactions.

FBgn0036534

DRSC - Results frm RNAi screens

FBgn0036534

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0036534

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0036534

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6169

Flygut - An atlas of the Drosophila adult midgut

FBgn0036534

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0036534

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC000167

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

RNA degradation

MIST (protein-protein) - An integrated Molecular Interaction Database

39722

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

mRNA decay by 5' to 3' exoribonuclease

References (91)