FB2024_03 , released June 25, 2024
Allele: Scer\GAL430Y
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General Information
Symbol
Scer\GAL430Y
Species
S. cerevisiae
Name
FlyBase ID
FBal0048770
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
P{GawB}30Y
Carried in Construct
Also Known As
30Y-GAL4, 30Y, P[Gal4]30Y
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(Amrein and Axel, 1997, Yang et al., 1995)
Progenitor genotype
Associated Insertion(s)
P{GawB}30Y
Cytology
Description
Allele components
Component
Use(s)
Inserted element
P{GawB}
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      No Assay Recorded
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      adult mushroom body
      (Yang et al., 1995)
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      larval stage
      mushroom body calyx
      (Tettamanti et al., 1997)
      Kenyon cell | subset
      (Tettamanti et al., 1997)
      second instar larval stage -- third instar larval stage
      adult mushroom body alpha'-lobe | restricted
      (Tettamanti et al., 1997)
      adult mushroom body alpha-lobe | restricted
      (Tettamanti et al., 1997)
      mushroom body pedunculus
      (Tettamanti et al., 1997)
      third instar larval stage
      embryonic/larval mushroom body
      (Armstrong et al., 1998)
      inner layer of the larval mushroom body
      (Pauls et al., 2010, Pauls et al., 2010)
      outer layer of the larval mushroom body
      (Pauls et al., 2010, Pauls et al., 2010)
      core layer of the larval mushroom body
      (Pauls et al., 2010, Pauls et al., 2010)
      surface layer of the larval mushroom body
      (Pauls et al., 2010)
      pupal stage P5
      adult mushroom body alpha-lobe
      (Armstrong et al., 1998)
      pupal stage P5 -- P7
      mushroom body
      (Armstrong et al., 1998)
      adult mushroom body beta-lobe
      (Armstrong et al., 1998)
      pupal stage P6
      adult mushroom body alpha-lobe | restricted
      (Armstrong et al., 1998)
      pupal stage P6 -- P7
      adult mushroom body gamma-lobe
      (Armstrong et al., 1998)
      adult mushroom body alpha'-lobe

      Comment: Authors refer to the alpha'-lobe as the 'delta lobe'.

      (Armstrong et al., 1998)
      pupal stage P7
      adult mushroom body alpha-lobe
      (Armstrong et al., 1998)
      adult stage
      lobe system of mushroom body
      (Zars et al., 2000)
      ellipsoid body | restricted
      (Zars et al., 2000)
      protocerebrum | lateral | medial
      (Mehren and Griffith, 2004)
      adult median bundle | restricted
      (Zars et al., 2000)
      adult ellipsoid body ring neuron of ellipsoid body | subset
      (Zars et al., 2000)
      ellipsoid body | subset
      (Zars et al., 2000)
      fan-shaped body | restricted
      (Zars et al., 2000)
      large field neuron | subset of fan-shaped body
      (Zars et al., 2000)
      large field neuron | subset
      (Zars et al., 2000)
      adult optic lobe
      (Mehren and Griffith, 2004)
      gnathal ganglion | dorso-lateral
      (Mehren and Griffith, 2004)
      adult mushroom body alpha-lobe
      (Armstrong et al., 1998)
      adult mushroom body beta-lobe
      (Armstrong et al., 1998)
      adult mushroom body | restricted
      (Bushey et al., 2009, Tettamanti et al., 1997)
      adult mushroom body
      (Sakai and Kitamoto, 2006, Mehren and Griffith, 2004)
      adult antennal lobe
      (Mehren and Griffith, 2004)
      adult antennal lobe glomerulus | subset
      (Zars et al., 2000)
      adult optic lobe | restricted
      (Sakai and Kitamoto, 2006)
      adult gnathal ganglion | restricted
      (Sakai and Kitamoto, 2006)
      adult mushroom body gamma-lobe
      (Pauls et al., 2010, Aso et al., 2009, Armstrong et al., 1998)
      adult mushroom body alpha'-lobe
      (Pauls et al., 2010, Armstrong et al., 1998)
      adult pars intercerebralis | restricted
      (Crocker et al., 2010)
      adult brain | restricted
      (Crocker et al., 2010)
      mushroom body beta lobe core layer | intense
      (Aso et al., 2009)
      mushroom body alpha lobe core layer | intense
      (Aso et al., 2009)
      mushroom body beta lobe surface layer | intense
      (Aso et al., 2009)
      mushroom body alpha lobe surface layer | intense
      (Aso et al., 2009)
      mushroom body beta lobe posterior layer | intense
      (Aso et al., 2009)
      mushroom body alpha lobe posterior layer | intense
      (Aso et al., 2009)
      mushroom body beta' anterior-posterior layer
      (Aso et al., 2009)
      mushroom body alpha' middle layer
      (Aso et al., 2009)
      mushroom body beta' middle layer
      (Aso et al., 2009)
      mushroom body alpha' anterior-posterior layer
      (Aso et al., 2009)
      adult antennal lobe | faint
      (Aso et al., 2009)
      medulla
      (Aso et al., 2009)
      lobula
      (Aso et al., 2009)
      lobula plate
      (Aso et al., 2009)
      adult protocerebrum | restricted
      (Aso et al., 2009)
      adult lateral horn
      (Aso et al., 2009)
      anterior optic tubercle
      (Aso et al., 2009)
      inferior neuropils | faint
      (Aso et al., 2009)
      ventrolateral protocerebrum | faint
      (Aso et al., 2009)
      posterior lateral protocerebrum | faint
      (Aso et al., 2009)
      posterior slope | faint
      (Aso et al., 2009)
      adult antennal nerve | faint
      (Aso et al., 2009)
      adult deutocerebrum | faint
      (Aso et al., 2009)
      adult tritocerebrum
      (Aso et al., 2009)
      adult gnathal ganglion
      (Aso et al., 2009)
      alpha/beta Kenyon cell
      (Pauls et al., 2010)
      gamma Kenyon cell
      (Pauls et al., 2010)
      alpha'/beta' Kenyon cell
      (Pauls et al., 2010)
      adult pars intercerebralis
      (Aso et al., 2009)
      adult mushroom body alpha-lobe
      (Pauls et al., 2010)
      adult mushroom body beta-lobe
      (Pauls et al., 2010)
      adult mushroom body beta'-lobe
      (Pauls et al., 2010)
      adult stage | male
      labellar gland | restricted
      (Sakai and Kitamoto, 2006)
      adult labial sensory neuron | restricted
      (Sakai and Kitamoto, 2006)
      sensory neuron | subset of prothoracic tarsal segment
      (Sakai and Kitamoto, 2006)
      Additional Information
      Statement
      Reference

      Drives expression in mushroom body, and in central brain neuropils.

      (FlyBase Curators, 2017)

      ScerGAL430Y drives expression in the mushroom body, scattered expression int he optic lobe and subesophageal ganglion. Expression is also detected in the labellar glands, in both neuronal and non-neuronal cells, and in a subset of sensory neurons of the prothoracic male tarsus.

      (Sakai and Kitamoto, 2006)

      Adult expression is observed primarily in the mushroom bodies, subesophageal ganglion, antennal lobes, optic lobes, and lateral protocerebrum, but not in the chemosensory organs.

      (Mehren and Griffith, 2004)

      Adult expression is observed in a subset of the antennal glomeruli, in the lobes of mushroom body, in some ring neurons of the ellipsoid body, large field neurons of the fan-shaped body, and lateral fibers of the median bundle. Representative images can be seen Figure 4 of FBrf0127414.

      (Zars et al., 2000)

      Expression of ScerGAL430Y is extensive throughout the adult central brain neuropil. Expression is relatively extensive within the early and late pupal alpha-lobe, but is restricted in the mid-pupal stage. Expression of ScerGAL430Y is lost at pupal stage 6, but is observed again at pupal stage 7.

      (Armstrong et al., 1998)

      Larval expression is observed four Kenyon cells clusters, and in the calyx. In the second and third larval instars, expression is also seen in the pedunculus, in the β and γ' lobes of the mushroom body, and in the circumference of the α and α' lobes.

      (Tettamanti et al., 1997)
       
      Marker for
      adult optic lobe
      (Mehren and Griffith, 2004)
      antennal lobe
      (Mehren and Griffith, 2004)
      mushroom body
      (Mehren and Griffith, 2004)
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class

      abnormal behavior, with Mmus\PrkacamC.UAS

      (Joiner et al., 2006)

      abnormal behavior | heat sensitive, with shi1.UAS

      (Pitman et al., 2006)

      abnormal courtship behavior, with CaMKIIT287D.tetO, Tn10\tetRtTA.UAS

      (Mehren and Griffith, 2004)

      abnormal courtship behavior | male | heat sensitive, with shi1.UAS

      (Sakai and Kitamoto, 2006)

      abnormal courtship behavior | male | tetracycline conditional, with CaMKIIT287D.tetO, Scer\GAL80ChAT.3.3P, Tn10\tetRtTA.UAS

      (Mehren and Griffith, 2006)

      abnormal courtship behavior | male | tetracycline conditional, with CaMKIIT287D.tetO, Tn10\tetRtTA.UAS

      (Mehren and Griffith, 2006)

      abnormal learning | adult stage, with Hsap\AMPHUAS.Tag:HA

      (Zografos et al., 2016)

      abnormal learning | adult stage, with Hsap\FYNUAS.Tag:HA

      (Zografos et al., 2016)

      abnormal learning | adult stage, with Hsap\GNASUAS.Tag:HA

      (Zografos et al., 2016)

      abnormal learning | adult stage, with Hsap\PTNUAS.Tag:HA

      (Zografos et al., 2016)

      abnormal locomotor behavior | heat sensitive, with shi1.UAS

      (McGuire et al., 2001)

      abnormal locomotor behavior | male, with traUAS.cFa

      (Belgacem and Martin, 2002)

      abnormal memory, with painRNAi.UAS

      (Sakai et al., 2012)

      abnormal sleep, with AlkGD42

      (Bai and Sehgal, 2015)

      abnormal sleep, with AlkGD42, Scer\GAL80Mef2.mb247

      (Bai and Sehgal, 2015)

      abnormal sleep | adult stage, with Nf1KK101909

      (Machado Almeida et al., 2021)

      abnormal sleep | adult stage | female limited, with tra2GD768

      (Khericha et al., 2016)

      abnormal thermotaxis | chemical conditional, with Ecol\lacZUAS.cUa

      (Hong et al., 2008)

      decreased rate of movement | adult stage, with Octα2RHMC03079

      (Nakagawa et al., 2022)

      increased cell death, with Hsap\ATXN3tr.Q78.UAS.Tag:HA

      (Khurana et al., 2006)
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Inhibition of neural activity in Scer\GAL430Y-expressing neurons does not affect sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503). As in wild type flies, courtship defects are seen in response to (S,Z,Z)-CH503.

      (Shankar et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      NOT Enhanced by
      Suppressed by
      NOT suppressed by
      Enhancer of
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      GAL430Y
      Scer\GAL430Y
      Name Synonyms
      Secondary FlyBase IDs
        References (60)