FlyBase Allele Report: Dmel\msn[UAS.cSa]

General Information

Symbol

Dmel\msn^UAS.cSa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Su

FlyBase ID

FBal0092606

Feature type

allele

Associated gene

Dmel\msn

Associated Insertion(s)

Carried in Construct

P{UAS-msn.S}

Also Known As

UAS-msn, UAS-Misshapen, UAS-msn.S

Key Links

Transgenic product class

cDNA

(Su et al., 1998)

wild_type

(Su et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Su et al., 1998)

wild_type

(Su et al., 1998)

Mutagen

in vitro construct

(Lee et al., 2006, Su et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-msn.S}

Cytology

Description

UAS regulatory sequences drive expression of an msn cDNA.

(Su et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

msn

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates carcinoma

modeled by scrib¹

(Kong et al., 2021)

modeled by Ras85D^V12.UAS, scrib¹

(Kong et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal planar polarity, with Scer\GAL4^hs.2sev

(Paricio et al., 1999)

decreased size | adult stage, with Scer\GAL4^nub.PU

(Kong et al., 2021)

decreased size | larval stage | somatic clone - tissue specific, with Scer\GAL4^Act5C.PI

(Kong et al., 2021)

visible, with Scer\GAL4^hs.2sev

(Paricio et al., 1999)

Phenotype Manifest In

eye, with Scer\GAL4^hs.2sev

(Paricio et al., 1999)

eye disc | somatic clone - tissue specific, with Scer\GAL4^Act5C.PI

(Kong et al., 2021)

lamina, with Scer\GAL4^GMR.PF

(Ruan et al., 1999)

ommatidium, with Scer\GAL4^hs.2sev

(Paricio et al., 1999)

photoreceptor cell R1 & axon, with Scer\GAL4^GMR.PF

(Ruan et al., 1999)

photoreceptor cell R2 & axon, with Scer\GAL4^GMR.PF

(Ruan et al., 1999)

photoreceptor cell R3 & axon, with Scer\GAL4^GMR.PF

(Ruan et al., 1999)

photoreceptor cell R4 & axon, with Scer\GAL4^GMR.PF

(Ruan et al., 1999)

photoreceptor cell R5 & axon, with Scer\GAL4^GMR.PF

(Ruan et al., 1999)

photoreceptor cell R6 & axon, with Scer\GAL4^GMR.PF

(Ruan et al., 1999)

wing, with Scer\GAL4^nub.PU

(Kong et al., 2021)

Detailed Description

Statement

Reference

Eye disc-specific clones expressing msn^UAS.cSa under the control of Scer\GAL4^Act5C.PI are smaller than control clones.

The expression of msn^UAS.cSa under the control of Scer\GAL4^nub.PU induces a small wing.

(Kong et al., 2021)

Expression of msn^Scer\UAS.cSa in the wing, under the control of Scer\GAL4^Bx-MS1096 results in mild or undetectable phenotypic changes.

(Lee et al., 2006)

Over expression of msn^Scer\UAS.cSa in the eye driven by Scer\GAL4^hs.2sev causes a rough eye phenotype and reveals typical polarity defects in ommatidia.

(Paricio et al., 1999)

Expression of msn^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PF causes R1-R6 photoreceptor cell growth cones to show an "early stop" phenotype. Some growth cones terminate before others resulting in an uneven termination pattern within the lamina. The phenotype is dose dependent.

(Ruan et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Scer\GAL4^nub.PU, msn^UAS.cSa has decreased size | adult stage phenotype, non-suppressible by Scer\GAL4^nub.PU/ft^TRiP.cUa

(Kong et al., 2021)

Enhancer of

Statement

Reference

Scer\GAL4^Act5C.PI/msn^UAS.cSa is an enhancer of decreased size | larval stage | somatic clone - tissue specific phenotype of scrib¹

(Kong et al., 2021)

msn^UAS.cSa, Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Buff et al., 2007)

Scer\GAL4^sca-537.4/msn^UAS.cSa is an enhancer of visible phenotype of Rala^35d

(Balakireva et al., 2006)

NOT Enhancer of

Statement

Reference

msn^UAS.cSa, Scer\GAL4^Act5C.PI, msn³²⁰⁸, msn³²⁰⁸ is a non-enhancer of neoplasia | larval stage | somatic clone - tissue specific phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI

(Kong et al., 2021)

Suppressor of

Statement

Reference

msn^UAS.cSa, Scer\GAL4^Act5C.PI is a suppressor of increased size | larval stage | somatic clone - tissue specific phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI

(Kong et al., 2021)

msn^UAS.cSa, Scer\GAL4^Act5C.PI is a suppressor of increased size | larval stage | somatic clone - tissue specific phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI, scrib¹

(Kong et al., 2021)

Scer\GAL4^Act5C.PI/msn^UAS.cSa is a suppressor | partially of increased size | larval stage | somatic clone - tissue specific phenotype of Scer\GAL4^Act5C.PI, bsk^DN.UAS.cUa, scrib¹

(Kong et al., 2021)

Scer\GAL4^nub.PU/msn^UAS.cSa is a suppressor of increased size | adult stage phenotype of Scer\GAL4^nub.PU, ft^TRiP.cUa

(Kong et al., 2021)

NOT Suppressor of

Statement

Reference

msn^UAS.cSa, Scer\GAL4^Antp-10 is a non-suppressor of increased cell number | third instar larval stage phenotype of ND-75^KK108222, Scer\GAL4^Antp-10

(Sinenko et al., 2011)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Scer\GAL4^nub.PU, msn^UAS.cSa has wing phenotype, non-suppressible by Scer\GAL4^nub.PU/ft^TRiP.cUa

(Kong et al., 2021)

Scer\GAL4^elav-C155, msn^UAS.cSa has photoreceptor & axon phenotype, non-suppressible by dock⁰⁴⁷²³

(Su et al., 2000)

Enhancer of

Statement

Reference

Scer\GAL4^Act5C.PI/msn^UAS.cSa is an enhancer of eye disc | somatic clone - tissue specific phenotype of scrib¹

(Kong et al., 2021)

msn^UAS.cSa, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Buff et al., 2007)

Scer\GAL4^sca-537.4/msn^UAS.cSa is an enhancer of microchaeta phenotype of Rala^35d

(Balakireva et al., 2006)

Scer\GAL4^sca-537.4/msn^UAS.cSa is an enhancer of macrochaeta phenotype of Rala^35d

(Balakireva et al., 2006)

msn^UAS.cSa, Scer\GAL4^Bx-MS1096 is an enhancer of wing blade phenotype of Lkb1^UAS.cLa, Scer\GAL4^Bx-MS1096

(Lee et al., 2006)

msn^UAS.cSa, Scer\GAL4^Bx-MS1096 is an enhancer of wing phenotype of Lkb1^UAS.cLa, Scer\GAL4^Bx-MS1096

(Lee et al., 2006)

NOT Enhancer of

Statement

Reference

msn^UAS.cSa, Scer\GAL4^Act5C.PI, msn³²⁰⁸, msn³²⁰⁸ is a non-enhancer of eye disc | somatic clone - tissue specific phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI

(Kong et al., 2021)

Suppressor of

Statement

Reference

Scer\GAL4^Act5C.PI/msn^UAS.cSa is a suppressor | partially of eye disc | somatic clone - tissue specific phenotype of Scer\GAL4^Act5C.PI, bsk^DN.UAS.cUa, scrib¹

(Kong et al., 2021)

Scer\GAL4^nub.PU/msn^UAS.cSa is a suppressor of wing phenotype of Scer\GAL4^nub.PU, ft^TRiP.cUa

(Kong et al., 2021)

msn^UAS.cSa, Scer\GAL4^Act5C.PI is a suppressor of eye disc | somatic clone - tissue specific phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI

(Kong et al., 2021)

NOT Suppressor of

Statement

Reference

msn^UAS.cSa, Scer\GAL4^Antp-10 is a non-suppressor of lamellocyte | third instar larval stage phenotype of ND-75^KK108222, Scer\GAL4^Antp-10

(Sinenko et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of msn^Scer\UAS.cSa does not suppress the increase in circulating lamellocytes seen when ND75^KK108222 is expressed under the control of Scer\GAL4^Antp-10.

(Sinenko et al., 2011)

Simultaneous expression of msn^Scer\UAS.cSa with lkb1^Scer\UAS.cLa (both under the control of Scer\GAL4^Bx-MS1096 strongly enhances the lkb1^Scer\UAS.cLa phenotype, resulting in severe disruption in the structure and size of the wing blades.

(Lee et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

msn^UAS.cSa/Scer\GAL4^elav.PLu rescues msn⁰³³⁴⁹

(Ruan et al., 2002)

msn^UAS.cSa/Scer\GAL4^69B rescues msn¹⁰²

(Su et al., 2000)

msn^UAS.cSa/Scer\GAL4^69B rescues msn¹⁷²

(Su et al., 2000)

msn^UAS.cSa/Scer\GAL4^69B rescues msn¹⁰²

(Su et al., 1998)

Comments

When msn^-CT.Scer\UAS is driven by Scer\GAL4^69B in msn¹⁰² or msn¹⁷² embryos, 100% of rescued embryos survive to pupal stages, the dorsal closure phenotype seen in these mutants is also rescued.

(Su et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (2)

5946

108496

Notes on Origin