FlyBase Allele Report: Dmel\amos[UAS.cGa]

General Information

Symbol

Dmel\amos^UAS.cGa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Goulding

FlyBase ID

FBal0104677

Feature type

allele

Associated gene

Dmel\amos

Associated Insertion(s)

Carried in Construct

P{UAS-amos.G}

Also Known As

UAS-amos

Key Links

Transgenic product class

wild_type

(Goulding et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Goulding et al., 2000)

Mutagen

in vitro construct

(Goulding et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-amos.G}

Cytology

Description

UASt regulatory sequences drive expression of the amos open reading frame.

(Goulding et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

amos

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^ap-md544

(Villa-Cuesta et al., 2003)

lethal, with Scer\GAL4^Bx-MS1096

(Villa-Cuesta et al., 2003)

lethal, with Scer\GAL4^en-e16E

(Villa-Cuesta et al., 2003)

lethal, with Scer\GAL4^pnr-MD237

(Villa-Cuesta et al., 2003)

lethal, with Scer\GAL4^tsh-md621

(Villa-Cuesta et al., 2003)

lethal - all die before end of larval stage, with Scer\GAL4^dac-p7d23

(Chanut et al., 2002)

lethal - all die before end of larval stage, with Scer\GAL4^dpp.blk1

(Chanut et al., 2002)

lethal - all die before end of larval stage, with Scer\GAL4^h-H10

(Chanut et al., 2002)

lethal - all die before end of P-stage, with Scer\GAL4^C-765

(Villa-Cuesta et al., 2003)

lethal - all die before end of pupal stage, with Scer\GAL4^Bx-MS1096

(Lai, 2003)

lethal - all die before end of pupal stage, with Scer\GAL4^dpp.blk1

(Lai, 2003)

some die during larval stage, with Scer\GAL4^dac-p7d23

(Chanut et al., 2002)

some die during larval stage, with Scer\GAL4^dpp.blk1

(Chanut et al., 2002)

some die during larval stage, with Scer\GAL4^h-H10

(Chanut et al., 2002)

some die during pharate adult stage, with Scer\GAL4^C-765

(Villa-Cuesta et al., 2003)

some die during pupal stage, with Scer\GAL4^Bx-MS1096

(Lai, 2003)

some die during pupal stage, with Scer\GAL4^dpp.blk1

(Lai, 2003)

viable, with Scer\GAL4^GMR.12

(Chanut et al., 2002)

visible, with Scer\GAL4^C-765

(Villa-Cuesta et al., 2003)

visible, with Scer\GAL4^hs.PB

(Lai, 2003, Goulding et al., 2000)

visible, with Scer\GAL4^OK384

(Goulding et al., 2000)

visible, with Scer\GAL4^sca-109-68

(Goulding et al., 2000)

visible | heat sensitive, with Scer\GAL4^hs.PB

(Chanut et al., 2002)

Phenotype Manifest In

adult head & olfactory sensory organ | ectopic, with Scer\GAL4^sca-109-68

(Goulding et al., 2000)

adult thorax & macrochaeta, with Scer\GAL4^sca-109-68

(Goulding et al., 2000)

adult thorax & olfactory sensory organ | ectopic, with Scer\GAL4^sca-109-68

(Goulding et al., 2000)

antenna, with Scer\GAL4^OK384

(Goulding et al., 2000)

antennal segment 1 & olfactory sensory organ | ectopic, with Scer\GAL4^hs.PB

(Goulding et al., 2000)

antennal segment 2 & olfactory sensory organ | ectopic, with Scer\GAL4^hs.PB

(Goulding et al., 2000)

antennal sense organ, with Scer\GAL4^OK384

(Goulding et al., 2000)

antennal sense organ | ectopic, with Scer\GAL4^OK384

(Goulding et al., 2000)

basiconic sensillum of antennal segment 3 | ectopic, with Scer\GAL4^OK384

(Goulding et al., 2000)

campaniform sensillum | ectopic, with Scer\GAL4^hs.PB

(Lai, 2003)

coeloconic sensillum of antennal segment 3 | ectopic, with Scer\GAL4^OK384

(Goulding et al., 2000)

macrochaeta, with Scer\GAL4^sca-109-68

(zur Lage et al., 2003)

macrochaeta | ectopic, with Scer\GAL4^dpp.blk1

(Lai, 2003)

macrochaeta | ectopic & adult head, with Scer\GAL4^C-765

(Villa-Cuesta et al., 2003)

macrochaeta | ectopic & mesothoracic tergum, with Scer\GAL4^C-765

(Villa-Cuesta et al., 2003)

mechanosensory chaeta | ectopic, with Scer\GAL4^hs.PB

(Lai, 2003)

microchaeta & adult thorax, with Scer\GAL4^sca-109-68

(Goulding et al., 2000)

ommatidium, with Scer\GAL4^hs.PB

(Chanut et al., 2002)

scutellum & chordotonal organ | ectopic, with Scer\GAL4^sca-109-68

(Goulding et al., 2000)

sense organ | ectopic, with Scer\GAL4^sca-109-68

(Goulding et al., 2000)

sensillum campaniformium | ectopic & wing, with Scer\GAL4^C-765

(Villa-Cuesta et al., 2003)

sensory mother cell | ectopic & dorsal mesothoracic disc, with Scer\GAL4^dpp.blk1

(Lai, 2003)

trichoid sensillum of antennal segment 3 | ectopic, with Scer\GAL4^OK384

(Goulding et al., 2000)

wing disc, with Scer\GAL4^dpp.blk1

(Lai, 2003)

wing sensillum | ectopic, with Scer\GAL4^C-765

(Villa-Cuesta et al., 2003)

wing vein L3 & chordotonal organ | ectopic, with Scer\GAL4^sca-109-68

(Goulding et al., 2000)

wing vein L3 & external sensory organ | ectopic, with Scer\GAL4^sca-109-68

(Goulding et al., 2000)

wing vein L3 & olfactory sensory organ | ectopic, with Scer\GAL4^sca-109-68

(Goulding et al., 2000)

Detailed Description

Statement

Reference

Expression of amos^Scer\UAS.cGa under the control of Scer\GAL4^dpp.blk1 results in a significant increase in the size of the wing disc. Ectopic sensory organ precursor cells are formed in the disc. These animals occasionally develop to pharate adults at 18^oC and in this case they have a large number of ectopic macrochaetae. Expression of amos^Scer\UAS.cGa under the control of Scer\GAL4^hs.PB using a 6 minute heat shock at 38^oC results in the induction of mechanosensory organs and campaniform sensilla in the wing.

(Lai, 2003)

Animals expressing amos^Scer\UAS.cGa under the control of Scer\GAL4^C-765 can develop to pharate adults at 17^oC. Their bodies are covered with many sensory organs of different types. These are mostly macrochaetae on the head and on the notum. Large numbers of sensilla campaniformia appear in the proximal wing and the rest of the wing contains a mixture of bristles of diverse types and sensilla campaniformia.

(Villa-Cuesta et al., 2003)

When amos^Scer\UAS.cGa is driven by Scer\GAL4^sca-109-68 there is a dramatic reduction in bristle formation.

(zur Lage et al., 2003)

Flies expressing amos^Scer\UAS.cGa under the control of Scer\GAL4^GMR.12 have no eye defect. Flies expressing amos^Scer\UAS.cGa under the control of Scer\GAL4^hs.PB (expressed using a 30 minute 37^oC heat shock at the beginning of the third larval instar) results in a rough eye phenotype. Third instar eye discs have irregularly spaced R8 precursors and occasional R8 clusters.

(Chanut et al., 2002)

Expression of amos^Scer\UAS.cGa under the control of Scer\GAL4^OK384 results in a dramatic increase in olfactory sensilla, with an increase of 24-44% for all sensillum types. A proportion of the sensilla appear to be of intermediate basiconica/trichodea morphology (they are shaped like sensilla basiconica but are more pigmented like sensilla trichodea). The antenna is bloated. Expression of amos^Scer\UAS.cGa under the control of Scer\GAL4^sca-109-68 results in a mixture of ectopic sense organs. Ectopic chordotonal organs are formed in the scutellum and third wing vein. Some extra external sense organs are formed, mostly along the third wing vein. In addition, unusual organs that appear morphologically similar to olfactory sensilla (particularly sensilla trichodea) are induced in the thorax, head and along the third wing vein. At high expression levels of amos, some of the sensory bristles of the thorax are transformed to olfactory-like morphology; while wild-type thoracic bristles are innervated by a single sensory neuron, a small proportion of these transformed sensilla have two or more sensory neurons, a characteristic of olfactory sensilla. Expression of amos^Scer\UAS.cGa under the control of Scer\GAL4^hs.PB results in a large number of ectopic olfactory-like sensilla on the antenna.

(Goulding et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

amos^UAS.cGa is a suppressor of visible | recessive phenotype of Scer\GAL4^hs.PB, lz³

(Goulding et al., 2000)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^hs.2sev/amos^UAS.cGa is an enhancer of eye phenotype of Scer\GAL4^hs.2sev, mbl^C.UAS

(Vicente-Crespo et al., 2008)

Suppressor of

Statement

Reference

amos^UAS.cGa/Scer\GAL4^hs.PB is a suppressor | partially of basiconic sensillum phenotype of Scer\GAL4^hs.PB, lz³

(Goulding et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

The reduction in number of sensilla basiconica seen in lz³ flies is partially rescued by expression of amos^Scer\UAS.cGa under the control of Scer\GAL4^hs.PB.

(Goulding et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

amos^UAS.cGa/Scer\GAL4^amos.PZ partially rescues amos¹/Df(2L)M36F-S6

(Holohan et al., 2006)

Comments

Expression of amos^Scer\UAS.cGa under the control of Scer\GAL4^amos.PZ substantially rescues the mutant phenotype seen in the sensilla of the third antennal segment in amos¹/Df(2L)M36F-S6 flies; ectopic bristles are almost completely suppressed in the rescued flies, and sensilla trichodea are present in numbers close to wild type. Substantial numbers of sensilla basiconica are also present in the rescued flies, but the number is less than half that expected for wild type.

(Holohan et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

51668

w^*; P{UAS-amos.G}5

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Roi^Scer\UAS.cGa

amos^Scer\UAS.cGa

amos^UAS.cGa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Goulding

(Goulding et al., 2000)

Secondary FlyBase IDs

References (7)

Report Sections

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