FlyBase Allele Report: Dmel\Atg18a[KG03090]

General Information

Symbol

Dmel\Atg18a^KG03090

Species

D. melanogaster

Name

FlyBase ID

FBal0134302

Feature type

allele

Associated gene

Dmel\Atg18a

Associated Insertion(s)

P{SUPor-P}Atg18a^KG03090

Carried in Construct

Also Known As

atg18^KG03090

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{SUPor-P}Atg18a^KG03090

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:8,187,376..8,187,376 [-]

(Kiral et al., 2020, Billes et al., 2018, Nandy et al., 2018, Pircs et al., 2012, Tian et al., 2011, Shen and Ganetzky, 2009, Scott et al., 2004, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Atg18^KG03090 mutants display basal autophagy defects in larval fat body cells.

(Pircs et al., 2012)

Atg18^KG03090 heterozygous neuromuscular junctions exhibit wild-type bouton levels.

(Tian et al., 2011)

Atg18^KG03090/Df(3L)Exel6112 animals analysed at +4 hours relative to puparium formation show a significant delay in midgut histolysis compared to controls. At +12 hours relative to puparium formation, larval midgut contraction is dramatically delayed in the mutants compared to controls.

(Denton et al., 2009)

Larvae homozygous for Atg18^KG03090 show abnormal autophagic response to starvation.

(Köhler et al., 2009)

Atg18^KG03090/+ mutants exhibit a neuromuscular junction undergrowth phenotype but do not exhibit defects in axonal transport.

(Shen and Ganetzky, 2009)

At 24 hours after puparium formation, Atg18^KG03090/Df(3L)66C-G28 animals expressing still contain vacuolated salivary gland fragments, in contrast to wild-type animals, where the salivary glands have completely degraded by this stage.

DNA fragmentation is detected by TUNEL in salivary glands of Atg18^KG03090/Df(3L)Exel6112 animals at 13.5 hours after puparium formation, as occurs in control salivary glands at this stage.

(Berry and Baehrecke, 2007)

Mutants show disruption of autophagy.

(Scott et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Atg18a^KG03090/Atg18[+] is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, bchs^EP2299

(Simonsen et al., 2007)

Suppressor of

Statement

Reference

Atg18a^KG03090, mask^UAS.cZa, Atg18a[+], Scer\GAL4^how-24B is a suppressor | partially of lethal - all die before end of P-stage | heat sensitive phenotype of Prosβ6^1.B.UAS, Scer\GAL4^how-24B

(Zhu et al., 2017)

NOT Suppressor of

Statement

Reference

Atg18a^KG03090, mask^UAS.cZa, Atg18a[+], Scer\GAL4^GMR.PU is a non-suppressor of visible | adult stage phenotype of Hsap\MAPT^UAS.GFP, Scer\GAL4^GMR.PU

(Zhu et al., 2017)

Atg18a^KG03090, mask^UAS.cZa, Atg18a[+], Scer\GAL4^GMR.PU is a non-suppressor of visible | adult stage phenotype of Hsap\FUS^{R521H.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Zhu et al., 2017)

Atg18a^KG03090, mask^UAS.cZa, Atg18a[+], Scer\GAL4^GMR.PU is a non-suppressor of visible | adult stage phenotype of Hsap\FUS^{R518K.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Zhu et al., 2017)

Phenotype Manifest In

Enhancer of

Statement

Reference

Atg18a^KG03090/Atg18[+] is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, bchs^EP2299

(Simonsen et al., 2007)

NOT Enhancer of

Statement

Reference

Atg18a^KG03090/Atg18a[+] is a non-enhancer of adult external thorax phenotype of park^Δ21/park²⁵

(Zhu et al., 2015)

Suppressor of

Statement

Reference

Atg18a^KG03090, mask^UAS.cZa, Atg18a[+], Scer\GAL4^how-24B is a suppressor | partially of pupal thorax | heat sensitive phenotype of Prosβ6^1.B.UAS, Scer\GAL4^how-24B

(Zhu et al., 2017)

Atg18a^KG03090/Atg18a[+] is a suppressor of adult external thorax phenotype of mask^10.22, park^Δ21/park²⁵

(Zhu et al., 2015)

Atg18a^KG03090/Atg18[+] is a suppressor of synapse phenotype of Rae1^EX28

(Tian et al., 2011)

Atg18a^KG03090/Atg18[+] is a suppressor of NMJ bouton phenotype of Rae1^EX28

(Tian et al., 2011)

NOT Suppressor of

Statement

Reference

Atg18a^KG03090, mask^UAS.cZa, Atg18a[+], Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Hsap\MAPT^UAS.GFP, Scer\GAL4^GMR.PU

(Zhu et al., 2017)

Atg18a^KG03090, mask^UAS.cZa, Atg18a[+], Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Hsap\FUS^{R521H.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Zhu et al., 2017)

Atg18a^KG03090, mask^UAS.cZa, Atg18a[+], Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Hsap\FUS^{R518K.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Zhu et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The indentations on the thorax phenotype characteristic for adult park²⁵/park^Δ21 transheterozygotes is not changed by combination with Atg18a^KG03090 in heterozygous state: the penetrance of the indentation phenotype is comparable to park²⁵/park^Δ21 mutants alone.

The partial suppression of the indentations on the thorax phenotype characteristic for adult park²⁵/park^Δ21 transheterozygotes that results from combination with mask^10.22 allele in heterozygous state is reverted again by addition of Atg18a^KG03090 in heterozygous state: the penetrance of the indentation phenotype in park²⁵/park^Δ21;mask^10.22/+;Atg18a^KG03090/+ triple mutants is comparable to park²⁵/park^Δ21 mutants alone.

(Zhu et al., 2015)

An Atg18^KG03090 heterozygous background dominantly suppresses the increase in bouton number seen in Rae1^EX28 homozygous mutant neuromuscular junction.

(Tian et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

13945

y¹; P{SUPor-P}Atg18a^KG03090 ry⁵⁰⁶/TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Atg18^KG03090

(Nandy et al., 2018, Nagy et al., 2014, Chang et al., 2013, Denton et al., 2009, Köhler et al., 2009)

Atg18[KG03090]

(Nagy et al., 2015)

Atg18a^KG03090

(Nandy et al., 2018)

CG7986^KG03090

atg18^KG03090

(Zhu et al., 2017, Zhu et al., 2015, Milton et al., 2011, Shen and Ganetzky, 2009, Berry and Baehrecke, 2007, Simonsen et al., 2007)

atg18a^KG03090

(Kiral et al., 2020, Connolly et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (19)

Report Sections

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