FlyBase Allele Report: Dmel\mask[UAS.cZa]

General Information

Symbol

Dmel\mask^UAS.cZa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Zhu

FlyBase ID

FBal0319554

Feature type

allele

Associated gene

Dmel\mask

Associated Insertion(s)

Carried in Construct

P{UAS-mask.Z}

Also Known As

UAS-Mask

Key Links

Transgenic product class

cDNA

(Zhu et al., 2015)

wild_type

(Zhu et al., 2015)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Zhu et al., 2015)

wild_type

(Zhu et al., 2015)

Mutagen

in vitro construct

(Zhu et al., 2015)

Progenitor genotype

Carried in construct

P{UAS-mask.Z}

Cytology

Description

UASt regulatory sequences drive expression of a full-length mask cDNA.

(Zhu et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

mask

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates tauopathy

modeled by Hsap\MAPT^UAS.GFP

(Zhu et al., 2017)

ameliorates amyotrophic lateral sclerosis type 6

modeled by Hsap\FUS^{R521H.UAS.Tag:HA}

(Zhu et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | third instar larval stage

(Zhu et al., 2017)

Phenotype Manifest In

autolysosome | third instar larval stage, with Scer\GAL4^how-24B

(Zhu et al., 2017)

autophagosome | third instar larval stage, with Scer\GAL4^how-24B

(Zhu et al., 2017)

embryonic/larval somatic muscle cell | third instar larval stage, with Scer\GAL4^how-24B

(Zhu et al., 2017, Zhu et al., 2015)

mitochondrion | third instar larval stage, with Scer\GAL4^how-24B

(Zhu et al., 2015)

Detailed Description

Statement

Reference

Expressing mask^UAS.cZa under the control of Scer\GAL4^how-24B results in significantly more and bigger autolysosomes around the nuclei (assessed by the red:green fluorescence ration of Atg8-GFP-mCherry puncta) in muscles throughout third instar larval stage than controls. There is no difference in autophagosome numbers and no change in pupal thoracic morphology and body size.

(Zhu et al., 2017)

Ectopic expression of mask^Scer\UAS.cZa under the control of Scer\GAL4^how-24B results in more densely tangled mitochondrial network compared to wild-type control.

(Zhu et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

mask^UAS.cZa/Scer\GAL4^GMR.PU is an enhancer of visible | adult stage phenotype of Atg1^UAS.cSa

(Zhu et al., 2017)

Suppressor of

Statement

Reference

mask^UAS.cZa/Scer\GAL4^ey.PU is a suppressor of abnormal size | adult stage phenotype of Scer\GAL4^ey.PU, mir-285^{UAS.DsRed(Unk)}

(Li et al., 2017)

mask^UAS.cZa/Scer\GAL4^ey.PU is a suppressor of visible | adult stage phenotype of Scer\GAL4^ey.PU, mir-285^{UAS.DsRed(Unk)}

(Li et al., 2017)

mask^UAS.cZa/Scer\GAL4^GMR.PU is a suppressor of visible | adult stage phenotype of Scer\GAL4^hh.PU, mir-285^{UAS.DsRed(Unk)}

(Li et al., 2017)

mask^UAS.cZa/Scer\GAL4^en.PU is a suppressor of increased cell death | larval stage phenotype of Scer\GAL4^en.PU, mir-285^{UAS.DsRed(Unk)}

(Li et al., 2017)

mask^UAS.cZa, Scer\GAL4^GMR.PU is a suppressor | partially of visible | adult stage phenotype of Hsap\MAPT^UAS.GFP, Scer\GAL4^GMR.PU

(Zhu et al., 2017)

mask^UAS.cZa, Scer\GAL4^GMR.PU is a suppressor | partially of visible | adult stage phenotype of Hsap\FUS^{R521H.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Zhu et al., 2017)

mask^UAS.cZa, Scer\GAL4^GMR.PU is a suppressor | partially of visible | adult stage phenotype of Hsap\FUS^{R518K.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Zhu et al., 2017)

mask^UAS.cZa, Scer\GAL4^how-24B is a suppressor of lethal - all die before end of P-stage | heat sensitive phenotype of Prosβ6^1.B.UAS, Scer\GAL4^how-24B

(Zhu et al., 2017)

mask^UAS.cZa, Scer\GAL4^how-24B is a suppressor of decreased body size | P-stage | heat sensitive phenotype of Prosβ6^1.B.UAS, Scer\GAL4^how-24B

(Zhu et al., 2017)

Atg1²⁵, mask^UAS.cZa, Atg1[+], Scer\GAL4^how-24B is a suppressor | partially of lethal - all die before end of P-stage | heat sensitive phenotype of Prosβ6^1.B.UAS, Scer\GAL4^how-24B

(Zhu et al., 2017)

Atg2[+], mask^UAS.cZa, Atg2^EP3697, Scer\GAL4^how-24B is a suppressor | partially of lethal - all die before end of P-stage | heat sensitive phenotype of Prosβ6^1.B.UAS, Scer\GAL4^how-24B

(Zhu et al., 2017)

Atg18a^KG03090, mask^UAS.cZa, Atg18a[+], Scer\GAL4^how-24B is a suppressor | partially of lethal - all die before end of P-stage | heat sensitive phenotype of Prosβ6^1.B.UAS, Scer\GAL4^how-24B

(Zhu et al., 2017)

mask^UAS.cZa, Vha68-2[+], Vha68-2^EP2364, Scer\GAL4^how-24B is a suppressor | partially of lethal - all die before end of P-stage | heat sensitive phenotype of Prosβ6^1.B.UAS, Scer\GAL4^how-24B

(Zhu et al., 2017)

NOT Suppressor of

Statement

Reference

Atg18a^KG03090, mask^UAS.cZa, Atg18a[+], Scer\GAL4^GMR.PU is a non-suppressor of visible | adult stage phenotype of Hsap\FUS^{R518K.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Zhu et al., 2017)

mask^UAS.cZa, Vha55[+], Vha55¹⁴, Scer\GAL4^GMR.PU is a non-suppressor of visible | adult stage phenotype of Hsap\FUS^{R518K.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Zhu et al., 2017)

mask^UAS.cZa, Vha55[+], Vha55¹⁶, Scer\GAL4^GMR.PU is a non-suppressor of visible | adult stage phenotype of Hsap\FUS^{R518K.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Zhu et al., 2017)

Atg2[+], mask^UAS.cZa, Atg2^EP3697, Scer\GAL4^GMR.PU is a non-suppressor of visible | adult stage phenotype of Hsap\MAPT^UAS.GFP, Scer\GAL4^GMR.PU

(Zhu et al., 2017)

Atg18a^KG03090, mask^UAS.cZa, Atg18a[+], Scer\GAL4^GMR.PU is a non-suppressor of visible | adult stage phenotype of Hsap\MAPT^UAS.GFP, Scer\GAL4^GMR.PU

(Zhu et al., 2017)

mask^UAS.cZa, Vha55[+], Vha55¹⁶, Scer\GAL4^GMR.PU is a non-suppressor of visible | adult stage phenotype of Hsap\MAPT^UAS.GFP, Scer\GAL4^GMR.PU

(Zhu et al., 2017)

Atg18a^KG03090, mask^UAS.cZa, Atg18a[+], Scer\GAL4^GMR.PU is a non-suppressor of visible | adult stage phenotype of Hsap\FUS^{R521H.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Zhu et al., 2017)

Phenotype Manifest In

Enhancer of

Statement

Reference

mask^UAS.cZa/Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Atg1^UAS.cSa

(Zhu et al., 2017)

Suppressor of

Statement

Reference

mask^UAS.cZa/Scer\GAL4^ey.PU is a suppressor of eye phenotype of Scer\GAL4^ey.PU, mir-285^{UAS.DsRed(Unk)}

(Li et al., 2017)

mask^UAS.cZa/Scer\GAL4^GMR.PU is a suppressor of wing phenotype of Scer\GAL4^hh.PU, mir-285^{UAS.DsRed(Unk)}

(Li et al., 2017)

mask^UAS.cZa/Scer\GAL4^en.PU is a suppressor of wing disc phenotype of Scer\GAL4^en.PU, mir-285^{UAS.DsRed(Unk)}

(Li et al., 2017)

mask^UAS.cZa, Scer\GAL4^GMR.PU is a suppressor | partially of eye phenotype of Hsap\MAPT^UAS.GFP, Scer\GAL4^GMR.PU

(Zhu et al., 2017)

mask^UAS.cZa, Scer\GAL4^GMR.PU is a suppressor | partially of eye phenotype of Hsap\FUS^{R521H.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Zhu et al., 2017)

mask^UAS.cZa, Scer\GAL4^GMR.PU is a suppressor | partially of eye phenotype of Hsap\FUS^{R518K.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Zhu et al., 2017)

mask^UAS.cZa, Scer\GAL4^how-24B is a suppressor of pupal thorax | heat sensitive phenotype of Prosβ6^1.B.UAS, Scer\GAL4^how-24B

(Zhu et al., 2017)

Atg1²⁵, mask^UAS.cZa, Atg1[+], Scer\GAL4^how-24B is a suppressor | partially of pupal thorax | heat sensitive phenotype of Prosβ6^1.B.UAS, Scer\GAL4^how-24B

(Zhu et al., 2017)

Atg2[+], mask^UAS.cZa, Atg2^EP3697, Scer\GAL4^how-24B is a suppressor | partially of pupal thorax | heat sensitive phenotype of Prosβ6^1.B.UAS, Scer\GAL4^how-24B

(Zhu et al., 2017)

Atg18a^KG03090, mask^UAS.cZa, Atg18a[+], Scer\GAL4^how-24B is a suppressor | partially of pupal thorax | heat sensitive phenotype of Prosβ6^1.B.UAS, Scer\GAL4^how-24B

(Zhu et al., 2017)

mask^UAS.cZa, Vha68-2[+], Vha68-2^EP2364, Scer\GAL4^how-24B is a suppressor | partially of pupal thorax | heat sensitive phenotype of Prosβ6^1.B.UAS, Scer\GAL4^how-24B

(Zhu et al., 2017)

mask^UAS.cZa/Scer\GAL4^how-24B is a suppressor of mitochondrion | third instar larval stage phenotype of Scer\GAL4^how-24B, park^UAS.mCherry

(Zhu et al., 2015)

mask^UAS.cZa/Scer\GAL4^how-24B is a suppressor of embryonic/larval somatic muscle cell | third instar larval stage phenotype of Scer\GAL4^how-24B, park^UAS.mCherry

(Zhu et al., 2015)

NOT Suppressor of

Statement

Reference

Atg2[+], mask^UAS.cZa, Atg2^EP3697, Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Hsap\MAPT^UAS.GFP, Scer\GAL4^GMR.PU

(Zhu et al., 2017)

Atg18a^KG03090, mask^UAS.cZa, Atg18a[+], Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Hsap\MAPT^UAS.GFP, Scer\GAL4^GMR.PU

(Zhu et al., 2017)

mask^UAS.cZa, Vha55[+], Vha55¹⁶, Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Hsap\FUS^{R518K.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Zhu et al., 2017)

mask^UAS.cZa, Vha55[+], Vha55¹⁶, Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Hsap\MAPT^UAS.GFP, Scer\GAL4^GMR.PU

(Zhu et al., 2017)

Atg18a^KG03090, mask^UAS.cZa, Atg18a[+], Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Hsap\FUS^{R521H.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Zhu et al., 2017)

Atg18a^KG03090, mask^UAS.cZa, Atg18a[+], Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Hsap\FUS^{R518K.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Zhu et al., 2017)

mask^UAS.cZa, Vha55[+], Vha55¹⁴, Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Hsap\FUS^{R518K.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Zhu et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The mitochondrial fragmentation seen in body wall muscles in third instar larvae expressing park^{Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^how-24B is suppressed by co-expression of mask^Scer\UAS.cZa which leads to highly tangled and interconnected mitochondria.

(Zhu et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

mask^UAS.cZa rescues mask^10.22/Df(3R)BSC317

(Zhu et al., 2017)

mask^UAS.cZa/Scer\GAL4^how-24B rescues mask^10.22/Df(3R)BSC317

(Zhu et al., 2015)

Comments

Expressing mask^UAS.cZa under the control of Scer\GAL4^how-24B rescues the over-accumulation of autophagosomes and increases the amount of autolysosomes in somatic muscles from mask^10.22/Df(3R)BSC317 wandering third instar larvae.

(Zhu et al., 2017)

Ectopic expression of mask^Scer\UAS.cZa under the control of Scer\GAL4^how-24B rescues both the mitochondrial connectivity and distribution defects.

(Zhu et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

mask^Scer\UAS.cZa

mask^UAS.cZa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Zhu

Secondary FlyBase IDs

References (5)

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