FlyBase Allele Report: Hsap\MAPT[UAS.GFP]

General Information

Symbol

Hsap\MAPT^UAS.GFP

Species

H. sapiens

Name

FlyBase ID

FBal0323607

Feature type

allele

Associated gene

Hsap\MAPT

Associated Insertion(s)

Carried in Construct

P{UAS-hMAPT.GFP}

Key Links

Transgenic product class

wild_type

(Rui et al., 2015)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Rui et al., 2015)

Mutagen

in vitro construct

(Rui et al., 2015)

Progenitor genotype

Carried in construct

P{UAS-hMAPT.GFP}

Cytology

Description

UAS regulatory sequences drive expression of full-length Hsap\MAPT tagged at the C-terminal end with GFP.

(Rui et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Hsap\MAPT

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of tauopathy

CEA

(Zhu et al., 2017)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of tauopathy

is exacerbated by mask^HMS01045

(Zhu et al., 2017)

is ameliorated by mask^UAS.cZa

(Zhu et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death | adult stage, with Scer\GAL4^GMR.PU

(Zhu et al., 2017)

visible | adult stage, with Scer\GAL4^GMR.PU

(Zhu et al., 2017)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PU

(Zhu et al., 2017)

Detailed Description

Statement

Reference

Expressing Hsap\MAPT^UAS.GFP under the control of Scer\GAL4^GMR.PU causes a severe rough eye phenotype with necrotic scars.

(Zhu et al., 2017)

Expression of Hsap\MAPT^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^shot-OK307 has no adverse effect on the adult thorax morphology and structure, nor does it affect the thoracic skeletal muscles.

(Rui et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hsap\MAPT^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by mask^HMS01045, Scer\GAL4^GMR.PU

(Zhu et al., 2017)

Suppressed by

Statement

Reference

Hsap\MAPT^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, suppressible | partially by mask^UAS.cZa, Scer\GAL4^GMR.PU

(Zhu et al., 2017)

Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307, htt^98E2 has short lived phenotype, suppressible by htt^+m22.7/htt[+]

(Rui et al., 2015)

Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307, htt^98E2 has abnormal locomotor behavior | progressive phenotype, suppressible by htt^+m22.7/htt[+]

(Rui et al., 2015)

Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307, htt^98E2 has short lived phenotype, suppressible | partially by Hsap\HTT^16Q.FL.UAS/Scer\GAL4^arm.PS

(Rui et al., 2015)

Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307, htt^98E2 has abnormal locomotor behavior | progressive phenotype, suppressible | partially by Hsap\HTT^16Q.FL.UAS/Scer\GAL4^arm.PS

(Rui et al., 2015)

NOT suppressed by

Statement

Reference

Hsap\MAPT^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-suppressible by Atg2[+]/mask^UAS.cZa/Atg2^EP3697, Scer\GAL4^GMR.PU

(Zhu et al., 2017)

Hsap\MAPT^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-suppressible by Atg18a^KG03090/mask^UAS.cZa/Atg18a[+], Scer\GAL4^GMR.PU

(Zhu et al., 2017)

Hsap\MAPT^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-suppressible by mask^UAS.cZa/Vha55[+]/Vha55¹⁶, Scer\GAL4^GMR.PU

(Zhu et al., 2017)

Enhancer of

Statement

Reference

Scer\GAL4^shot-OK307/Hsap\MAPT^UAS.GFP is an enhancer of short lived phenotype of htt^98E2

(Rui et al., 2015)

Scer\GAL4^shot-OK307/Hsap\MAPT^UAS.GFP is an enhancer of abnormal locomotor behavior | progressive phenotype of htt^98E2

(Rui et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

Hsap\MAPT^UAS.GFP, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by mask^HMS01045, Scer\GAL4^GMR.PU

(Zhu et al., 2017)

Atg1[+]/Atg1^Δ3D, Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307 has adult thorax phenotype, enhanceable by htt^98E2/htt[+]

(Rui et al., 2015)

Atg1[+]/Atg1^Δ3D, Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307 has adult somatic muscle cell of thorax phenotype, enhanceable by htt^98E2/htt[+]

(Rui et al., 2015)

Suppressed by

Statement

Reference

Hsap\MAPT^UAS.GFP, Scer\GAL4^GMR.PU has eye phenotype, suppressible | partially by mask^UAS.cZa, Scer\GAL4^GMR.PU

(Zhu et al., 2017)

Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307, htt^98E2 has adult thorax phenotype, suppressible by htt^+m22.7/htt[+]

(Rui et al., 2015)

Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307, htt^98E2 has adult somatic muscle cell of thorax phenotype, suppressible by htt^+m22.7/htt[+]

(Rui et al., 2015)

Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307, htt^98E2 has adult thorax phenotype, suppressible | partially by Hsap\HTT^16Q.FL.UAS, Scer\GAL4^shot-OK307

(Rui et al., 2015)

Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307, htt^98E2 has adult somatic muscle cell of thorax phenotype, suppressible | partially by Hsap\HTT^16Q.FL.UAS, Scer\GAL4^shot-OK307

(Rui et al., 2015)

NOT suppressed by

Statement

Reference

Hsap\MAPT^UAS.GFP, Scer\GAL4^GMR.PU has eye phenotype, non-suppressible by Atg2[+]/mask^UAS.cZa/Atg2^EP3697, Scer\GAL4^GMR.PU

(Zhu et al., 2017)

Hsap\MAPT^UAS.GFP, Scer\GAL4^GMR.PU has eye phenotype, non-suppressible by Atg18a^KG03090/mask^UAS.cZa/Atg18a[+], Scer\GAL4^GMR.PU

(Zhu et al., 2017)

Hsap\MAPT^UAS.GFP, Scer\GAL4^GMR.PU has eye phenotype, non-suppressible by mask^UAS.cZa/Vha55[+]/Vha55¹⁶, Scer\GAL4^GMR.PU

(Zhu et al., 2017)

Other

Statement

Reference

Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307, htt^98E2 has adult thorax phenotype

(Rui et al., 2015)

Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307, htt^98E2 has adult somatic muscle cell of thorax phenotype

(Rui et al., 2015)

Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307, htt^98E2/htt[+], ref(2)P^c03993/ref(2)P[+] has adult thorax phenotype

(Rui et al., 2015)

Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307, htt^98E2/htt[+], ref(2)P^c03993/ref(2)P[+] has adult somatic muscle cell of thorax phenotype

(Rui et al., 2015)

Atg8a^d4/Atg8a[+], Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307, htt^98E2/htt[+] has adult thorax phenotype

(Rui et al., 2015)

Atg8a^d4/Atg8a[+], Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307, htt^98E2/htt[+] has adult somatic muscle cell of thorax phenotype

(Rui et al., 2015)

Atg8a^d4, Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307 has adult thorax phenotype

(Rui et al., 2015)

Atg8a^d4, Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307 has adult somatic muscle cell of thorax phenotype

(Rui et al., 2015)

Atg1[+]/Atg1^Δ3D, Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307 has adult thorax phenotype

(Rui et al., 2015)

Atg1[+]/Atg1^Δ3D, Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307 has adult somatic muscle cell of thorax phenotype

(Rui et al., 2015)

Atg13^Δ81/Atg13[+], Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307, htt^98E2/htt[+] has adult somatic muscle cell of thorax phenotype

(Rui et al., 2015)

Atg7[+]/Atg7^d77, Hsap\MAPT^UAS.GFP, Scer\GAL4^shot-OK307, htt^98E2/htt[+] has adult somatic muscle cell of thorax phenotype

(Rui et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\MAPT^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^shot-OK307 in the htt^98E2 homozygous mutant background induces prominent collapse of the thorax due to severe loss of the muscles below, these phenotypes are not observed in either the Hsap\MAPT^{Scer\UAS.T:Avic\GFP} expressing flies or the htt^98E2 mutants alone and can be rescued by combination with a single copy of htt^+m22.7 or by co-expression of Hsap\HTT^{16Q.FL.Scer\UAS}. Expression of Hsap\MAPT^{Scer\UAS.T:Avic\GFP} in either htt^98E2 or Atg8a^d4 heterozygous background does not produce any obvious phenotype in the adult thorax. However, when Hsap\MAPT^{Scer\UAS.T:Avic\GFP} is expressed in either htt^98E2/+;Atg8a^d4/+ double heterozygotes or Atg8a^d4 homozygotes nearly all the adult flies have collapsed thoraces and display loss of skeletal thoracic muscles. Expression of Hsap\MAPT^{Scer\UAS.T:Avic\GFP} in Atg1^Δ3D/+ heterozygous background leads to moderate thoracic collapse and loss of skeletal muscles, this phenotype is strongly enhanced by combination with a single copy of htt^98E2.

Hsap\MAPT^{Scer\UAS.T:Avic\GFP} expression in adults heterozygous for any of the following alleles: Atg13^Δ81, Atg7^d77 or ref(2)P^c03993 has no phenotype, unless the animals are also heterozygous for htt^98E2, in which case a severe loss of thoracic somatic muscles is observed.

Co-expression of either of the temperature sensitive Prosβ6^1.B.Scer\UAS or Prosβ2^1.Scer\UAS alleles together with Hsap\MAPT^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^shot-OK307 has no adverse effect on the morphology and structure of the adult thorax or the thoracic somatic muscles below at either the non-permissive (28[o]C) or the permissive (22[o]C) temperatures.

The age-dependent loss of climbing ability as well as the reduced lifespan characteristic for htt^98E2 homozygous adults is further worsened by expression of Hsap\MAPT^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^shot-OK307 in the mutant background. This worsened phenotype can in turn be fully rescued by combination with a single copy of htt^+m22.7 and partially rescued by co-expression of Hsap\HTT^{16Q.FL.Scer\UAS}.

(Rui et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Hsap\MAPT^{Scer\UAS.T:Avic\GFP}

Hsap\MAPT^UAS.GFP

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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