FB2024_03 , released June 25, 2024
Allele: Dmel\foxoUAS.cFa
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General Information
Symbol
Dmel\foxoUAS.cFa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Junger
FlyBase ID
FBal0151927
Feature type
allele
Associated gene
Dmel\foxo
Associated Insertion(s)
Carried in Construct
P{UAS-foxo.F}
Also Known As
UAS-dFOXO, UAS-foxo
Key Links
Transgenic product class
cDNA
(Junger et al., 2003)
wild_type
(Junger et al., 2003)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Junger et al., 2003)
wild_type
(Junger et al., 2003)
Mutagen
in vitro construct
(Junger et al., 2003)
Progenitor genotype
Carried in construct
P{UAS-foxo.F}
Cytology
Description

Expression of cDNA for foxo is governed by UASt regulatory sequences.

(Junger et al., 2003)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
foxo
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  Parkinson's disease
      modeled by park25
      (Tain et al., 2009)
      modeled by Fer2MB09480
      (Tas et al., 2018)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class

      abnormal adult locomotory behavior, with Scer\GAL4Switch1.106

      (Martínez Corrales et al., 2022)

      abnormal developmental rate, with Scer\GAL4Cg.PA

      (Zhao et al., 2021)

      abnormal feeding behavior, with Scer\GAL4Mhc.PW

      (Demontis and Perrimon, 2010)

      abnormal neuroanatomy | pharate adult stage, with Scer\GAL4CCAP.PP

      (Zhao et al., 2008)

      chemical resistant, with Scer\GAL4Switch1.106

      (Martínez Corrales et al., 2022)

      chemical resistant | RU486 conditional, with Scer\GAL4Switch1.106

      (Giannakou et al., 2004)

      decreased body size | larval stage, with Scer\GAL4Cg.PA

      (Zhao et al., 2021)

      decreased cell size | larval stage | somatic clone, with Scer\GAL4Act.PU

      (Zhao et al., 2021)

      decreased fecundity | female, with Scer\GAL4Switch1.106

      (Giannakou et al., 2004)

      decreased size | larval stage | somatic clone, with Scer\GAL4Act.PU

      (Zhao et al., 2021)

      decreased speed of aging, with Scer\GAL4Mef2.PR, Scer\GAL80ts.αTub84B

      (Demontis and Perrimon, 2010)

      decreased speed of aging, with Scer\GAL4Mhc.PW

      (Demontis and Perrimon, 2010)

      decreased speed of aging, with Scer\GAL4UAS.cHa, Scer\GAL4tin.cBa

      (Wessells et al., 2004)

      increased cell death, with Scer\GAL4ptc-559.1

      (Wang et al., 2014)

      lethal - all die before end of larval stage, with Scer\GAL4Act.PU

      (Hwangbo et al., 2004)

      long lived, with Scer\GAL4Ilp2.215-55, foxoΔ94

      (Alic et al., 2014)

      long lived, with Scer\GAL4Mhc.PW

      (Demontis and Perrimon, 2010)

      long lived, with Scer\GAL4Switch1.106, foxoΔ94

      (Martínez Corrales et al., 2022)

      long lived | female, with Scer\GAL4Switch1.106

      (Martínez Corrales et al., 2022)

      long lived | female | RU486 conditional, with Scer\GAL4Switch1.106

      (Giannakou et al., 2005, Giannakou et al., 2004)

      long lived | RU486 conditional, with Scer\GAL4Switch1.106

      (Alic et al., 2014)

      long lived | RU486 conditional, with Scer\GAL4Switch1.106, foxoΔ94

      (Alic et al., 2014)

      some die during larval stage, with Scer\GAL4Act.PU

      (Hwangbo et al., 2004)

      viable, with Scer\GAL4UAS.cHa, Scer\GAL4tin.cBa

      (Wessells et al., 2004)

      visible, with Scer\GAL4GMR.PF

      (Junger et al., 2003)

      wild-type | RU486 conditional, with Scer\GAL4Switch1.106

      (Hwangbo et al., 2004)
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The presence of RU486 from day 2 of adulthood extends the median lifespan of female flies expressing foxoScer\UAS.cFa under the control of Scer\GAL4Switch1.106 on average by 6%.

      The presence of RU486 from day 2 of adulthood extends the median lifespan of foxoΔ94 female flies expressing foxoScer\UAS.cFa under the control of Scer\GAL4Switch1.106 on average by 10%.

      Expressing foxoScer\UAS.cFa specifically in the mNSCs in the adult brain, using the Scer\GAL4Ilp2.215-55 driver, extends the lifespan of female flies in both wild-type and foxoΔ94 mutants.

      Induction of foxoScer\UAS.cFa expression in the gut and fat body, under the control of Scer\GAL4Switch1.106 enhances the climbing ability of female flies throughout their lifespan, observed as an increase in the proportion of high, or combined medium and high, climbers. This enhancement is seen in both wild-type and foxoΔ94 backgrounds.

      Induction of foxoScer\UAS.cFa expression in adulthood, under the control of Scer\GAL4Switch1.106 (and RU486) has no effect on fecundity in either wild-type or foxoΔ94 mutants, but has an effect on body weight and protein content.

      (Alic et al., 2014)

      Expression of foxoScer\UAS.cFa in the adult gut and fat body under the control of Scer\GAL4Switch1.106 (in the presence of RU486) results in an extended lifespan compared to controls.

      Expression of foxoScer\UAS.cFa specifically in the adult gut and under the control of Scer\GAL4TIGS-2 (in the presence of RU486) has no major effect on lifespan compared to controls. Stored triacylglycerol (TAG) levels are also reduced compared to controls. Gut integrity and feeding appear normal.

      (Alic et al., 2014)

      Ectopic expression of foxoScer\UAS.cFa in the developing wing, under the control of Scer\GAL4ptc-559.1 induces apoptosis and the subsequent loss of the wing anterior cross vein.

      (Wang et al., 2014)

      Motor neuronal overexpression of foxoScer\UAS.cFa under the control of Scer\GAL4Rapgap1-OK6 does not result in neuromuscular junction overgrowth. These larvae display a modest reduction in the number of microtubule loops compared with wild-type.

      (Nechipurenko and Broihier, 2012)

      Female re-mating behaviour is not altered in flies expressing foxoScer\UAS.cFa under the control of Scer\GAL4Switch1.106 24 hours after mating to wild-type flies.

      (Wigby et al., 2011)

      Expression of foxoScer\UAS.cFa under the control of Scer\GAL4Lsp2.PH rescues the increase in triglyceride levels and the heart defects normally caused by a high-fat diet.

      Expression of foxoScer\UAS.cFa under the simultaneous control of both Scer\GAL4Scer\UAS.cHa and Scer\GAL4tin.cBa does not rescue the increase in triglyceride levels caused by a high-fat diet. However, the heart defects normally caused by a high-fat diet are rescued in these animals.

      (Birse et al., 2010)

      Overexpression of foxoScer\UAS.cFa in muscles under the control of Scer\GAL4Mhc.PW does not affect developmental growth and differentiation (as estimated by body weight and sarcomere assembly). Overexpression of foxoScer\UAS.cFa under the control of Scer\GAL4Mhc.PW results in delayed accumulation of age-related protein aggregates in muscles.

      Overexpression of foxoScer\UAS.cFa in adult muscles under the control of Scer\GAL4Mef2.PR and Scer\GAL80ts.αTub84B significantly preserves muscle protein homeostasis, while the muscles of wild-type animals display increased accumulation of protein aggregates. Compared to syngenic controls, the age-related accumulation of protein aggregates in retinas, brains and the peripheral fat body of the abdomen is also decreased in flies overexpressing foxoScer\UAS.cFa under the control of Scer\GAL4Mhc.PW.

      Muscle functionality gradually decreases in wild-type aging flies, resulting in impaired climbing and flight ability. Overexpression of foxoScer\UAS.cFa under the control of Scer\GAL4Mhc.PW significantly preserves muscle strength during aging.

      Overexpression of foxoScer\UAS.cFa under the control of Scer\GAL4Mhc.PW in muscles is sufficient to significantly extend longevity compared with wild type by increasing the median and maximum life span of flies.

      Food intake of flies is decreased in response to overexpression of foxoScer\UAS.cFa in muscles via Scer\GAL4Mhc.PW.

      The body weight of adult flies overexpressing foxoScer\UAS.cFa under the control of Scer\GAL4Mhc.PW does not significantly differ from that of syngenic controls.

      There is a significant decrease in the hemolymph glucose concentration compared with syngenic control in flies overexpressing foxoScer\UAS.cFa under the control of Scer\GAL4Mhc.PW

      (Demontis and Perrimon, 2010)

      Expression of foxoScer\UAS.cFa under the control of Scer\GAL4Ccap.PP results in the loss of adult-specific neurite projections; many central neurites and efferents are lost.

      (Zhao et al., 2008)

      Females expressing foxoScer\UAS.cFa under the control of Scer\GAL4Switch1.106 (in the presence of RU486) show an increase in mean and maximum lifespan compared with controls.

      (Giannakou et al., 2005)

      Females expressing foxoScer\UAS.cFa under the control of Scer\GAL4Switch1.106 (in the presence of RU486) show an increase in median life-span and reduced fecundity compared to controls. These females also show increased resistance to paraquat. No effect on life-span is seen in males expressing foxoScer\UAS.cFa under the control of Scer\GAL4Switch1.106 (in the presence of RU486).

      (Giannakou et al., 2004)

      When foxoScer\UAS.cFa is driven by Scer\GAL4bun-Switch1.32 (in adults fed on mifepristone) the median lifespan can increased by as much as 56%.

      (Hwangbo et al., 2004)

      In contrast to wild-type flies, the stress-induced heart failure rate of foxoScer\UAS.cFa, when under the control of both Scer\GAL4tin.cBa and Scer\GAL4Scer\UAS.cHa, does not increase with age.

      (Wessells et al., 2004)

      When expression is driven by Scer\GAL4GMR.PF the eye is reduced and the ommatidial pattern disrupted.

      (Junger et al., 2003)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      NOT Enhanced by
      Suppressed by
      Statement
      Reference

      Scer\GAL4Switch1.106, foxoUAS.cFa has long lived | female phenotype, suppressible by morHMS01267/Scer\GAL4Switch1.106

      (Martínez Corrales et al., 2022)

      Scer\GAL4Switch1.106, foxoUAS.cFa has long lived | female phenotype, suppressible by osaHMS01738/Scer\GAL4Switch1.106

      (Martínez Corrales et al., 2022)

      Scer\GAL4Switch1.106, foxoUAS.cFa has long lived | female phenotype, suppressible by IswiHMS00628, Scer\GAL4Switch1.106

      (Martínez Corrales et al., 2022)

      Scer\GAL4Switch1.106, foxoUAS.cFa has abnormal adult locomotory behavior phenotype, suppressible by IswiHMS00628, Scer\GAL4Switch1.106

      (Martínez Corrales et al., 2022)

      Scer\GAL4Switch1.106, foxoUAS.cFa has long lived | female phenotype, suppressible by Etl1HMS00829, Scer\GAL4Switch1.106

      (Martínez Corrales et al., 2022)

      Scer\GAL4Switch1.106, foxoUAS.cFa has chemical resistant phenotype, suppressible by morHMS01267/Scer\GAL4Switch1.106

      (Martínez Corrales et al., 2022)

      Scer\GAL4Switch1.106, foxoUAS.cFa has chemical resistant phenotype, suppressible by IswiHMS00628, Scer\GAL4Switch1.106

      (Martínez Corrales et al., 2022)

      Scer\GAL4Switch1.106, foxoUAS.cFa has long lived | female phenotype, suppressible by domHMS00142/Scer\GAL4Switch1.106

      (Martínez Corrales et al., 2022)

      Scer\GAL4Switch1.106, foxoUAS.cFa has long lived | female phenotype, suppressible by Tip60HM05049/Scer\GAL4Switch1.106

      (Martínez Corrales et al., 2022)

      Scer\GAL4Switch1.106, foxoUAS.cFa has abnormal adult locomotory behavior phenotype, suppressible by morHMS01267/Scer\GAL4Switch1.106

      (Martínez Corrales et al., 2022)

      Scer\GAL4Act.PU, foxoUAS.cFa has decreased cell size | somatic clone | larval stage phenotype, suppressible by Scer\GAL4Act.PU/Xbp1UAS.s.Tag:V5

      (Zhao et al., 2021)

      Scer\GAL4Act.PU, foxoUAS.cFa has decreased size | somatic clone | larval stage phenotype, suppressible by Scer\GAL4Act.PU/Xbp1UAS.s.Tag:V5

      (Zhao et al., 2021)

      Scer\GAL4Cg.PA, foxoUAS.cFa has decreased body size | larval stage phenotype, suppressible | partially by Xbp1UAS.s.Tag:V5/Scer\GAL4Cg.PA

      (Zhao et al., 2021)

      Scer\GAL4Cg.PA, foxoUAS.cFa has abnormal developmental rate phenotype, suppressible | partially by Xbp1UAS.s.Tag:V5/Scer\GAL4Cg.PA

      (Zhao et al., 2021)

      Scer\GAL4Switch1.106, foxoUAS.cFa, pntP1.UAS has short lived phenotype, suppressible by aopACT.UAS/Scer\GAL4Switch1.106

      (Alic et al., 2014)

      Scer\GAL4TIGS-2, foxoUAS.cFa, pntP1.UAS has short lived phenotype, suppressible | partially by Scer\GAL4TIGS-2/aopACT.UAS

      (Alic et al., 2014)
      NOT suppressed by
      Enhancer of
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Other
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The expression of foxoUAS.cFa under the control of Scer\GAL4GMR58E02 partially suppresses the progressive decrease in the number of adult PAM neurons and the decreased adult locomotion capacity observed in Fer2MB09480 homozygotes, while further reducing their decreased lifespan.

      (Tas et al., 2018)

      Co-expression of foxoScer\UAS.cFa and aopHMS01256 in the adult midgut and fat body under the control of Scer\GAL4Switch1.106 (in the presence of RU486) results in a reduced lifespan compared to controls.

      Expression of foxoScer\UAS.cFa enhances the reduction in lifespan seen when pntP1.Scer\UAS is expressed in the adult midgut and fat body under the control of Scer\GAL4Switch1.106 (in the presence of RU486). This phenotype is completely suppressed upon expression of aopACT.Scer\UAS. Co-expression of foxoScer\UAS.cFa and pntP1.Scer\UAS also enhances the reduction in stored triacylglycerol (TAG) levels seen when either transgene is expressed alone. Gut integrity and feeding appear normal.

      Expression of foxoScer\UAS.cFa enhances the reduction in lifespan seen when pntP1.Scer\UAS is expressed in the adult midgut under the control of Scer\GAL4TIGS-2 (in the presence of RU486). This phenotype is partially suppressed upon expression of aopACT.Scer\UAS.

      Flies co-expressing pntP1.Scer\UAS and foxoScer\UAS.cFa under the control of Scer\GAL4Switch1.106 are starvation sensitive after 5 days in the presence of RU486, and this phenotype can be reversed upon expression of aopACT.Scer\UAS.

      (Alic et al., 2014)

      Expression of foxoScer\UAS.cFa driven by Scer\GAL4how-24B significantly partially suppresses flight and climbing defects as well as muscle degeneration and mitochondrial disruption in park25/park25 flies; foxoScer\UAS.cFa driven by Scer\GAL4ple.PF significantly suppresses loss of dopaminergic PPL1 neurons in 30 day old park25/park25 flies. Expression of foxoScer\UAS.cFa in a Pink1B9/Pink1B9 background results in lethality.

      (Tain et al., 2009)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      foxoUAS.cFa/Scer\GAL4Lk6-DJ634 rescues foxo25/foxo21

      (Zheng et al., 2007)
      Partially rescues

      foxoUAS.cFa/Scer\GAL4GMR58E02 partially rescues foxoΔ94

      (Tas et al., 2018)

      foxoUAS.cFa/Scer\GAL4Switch1.106 partially rescues foxoΔ94

      (Alic et al., 2014)

      Scer\GAL4Ilp2.215-55/foxoUAS.cFa partially rescues foxoΔ94

      (Alic et al., 2014)
      Fails to rescue

      foxoUAS.cFa/Scer\GAL4P2.4.Pdf fails to rescue foxo25/foxo21

      (Zheng et al., 2007)
      Comments

      The expression of foxoUAS.cFa under the control of Scer\GAL4GMR58E02 rescues the decreased number of dopaminergic PAM neurons in the adult brain but enhances the decreased lifespan presented by foxoΔ94 homozygotes.

      (Tas et al., 2018)

      The presence of RU486 from day 2 of adulthood extends the median lifespan of foxoΔ94 female flies expressing foxoScer\UAS.cFa under the control of Scer\GAL4Switch1.106 on average by 10%.

      Expressing foxoScer\UAS.cFa specifically in the mNSCs in the adult brain, using the Scer\GAL4Ilp2.215-55 driver, extends the lifespan of female flies in foxoΔ94 mutants.

      Induction of foxoScer\UAS.cFa expression in the gut and fat body, under the control of Scer\GAL4Switch1.106 enhances the climbing ability of female flies throughout their lifespan, observed as an increase in the proportion of high, or combined medium and high, climbers. This enhancement is seen in a foxoΔ94 background.

      Induction of foxoScer\UAS.cFa expression in adulthood, under the control of Scer\GAL4Switch1.106 (and RU486) has no effect on fecundity in foxoΔ94 mutants, but has an effect on body weight and protein content.

      (Alic et al., 2014)

      Expression of foxoScer\UAS.cFa under the control of Scer\GAL4Lk6-DJ634 rescues the circadian arrhythmia seen in foxo21/foxo25 mutants. Phototaxis is not affected by paraquat treatment in these mutants.

      (Zheng et al., 2007)
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      foxoScer\UAS.cFa
      foxoUAS.cFa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Junger
      (Junger et al., 2003)
      Secondary FlyBase IDs
        References (28)