FlyBase Allele Report: Dmel\Ten-a[GD8834]

General Information

Symbol

Dmel\Ten-a^GD8834

Species

D. melanogaster

Name

FlyBase ID

FBal0208755

Feature type

allele

Associated gene

Dmel\Ten-a

Associated Insertion(s)

Carried in Construct

P{GD8834}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD8834}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD8834

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^GH146

(Hong et al., 2012)

abnormal neuroanatomy, with Scer\GAL4^peb-GAL4

(Hong et al., 2012)

abnormal neuroanatomy | adult stage

(Mosca and Luo, 2014)

abnormal neuroanatomy | somatic clone, with Scer\GAL4^Mz19

(Hong et al., 2012)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

adult olfactory receptor neuron Or47b, with Scer\GAL4^Or47b.7.467

(Mosca and Luo, 2014)

presynaptic active zone | adult stage, with Scer\GAL4^Or47b.7.467

(Mosca and Luo, 2014)

synapse | adult stage, with Scer\GAL4^Or47b.7.467

(Mosca and Luo, 2014)

Detailed Description

Statement

Reference

The expression of Ten-a^GD8834 under the control of Scer\GAL4^Or47b.7.467 results in adult antennal lobe glomeruli VA1lm with normal morphology, but the associated olfactory receptor neurons Or47b have significant decreases in the number and density of pre-synaptic sites, as assessed by an active zone marker, as compared to controls.

(Mosca and Luo, 2014)

Expression of Ten-a^GD8834 in the olfactory receptor neurons (ORNs) under the control of Scer\GAL4^peb-GAL4 results in very mild intermingling of Ncra\QF^Mz19.PH-expressing projection neuron (PN) dendrites and Or47b-expressing ORN axons (these axons and dendrites do not overlap in wild type).

Expression of Ten-a^GD8834 in the PNs (under the control of Scer\GAL4^GH146) results in intermingling of Ncra\QF^Mz19.PH-expressing PN dendrites and Or47b-expressing ORN axons.

Expression of Ten-a^GD8834 in DA1 projection neurons under the control of Scer\GAL4^Mz19 (using MARCM) causes their dendrites to mismatch with Or47b-expressing ORN axons. Expression of Ten-a^GD8834 in VA1d/DC3 projection neurons under the control of Scer\GAL4^Mz19 (using MARCM) does not result in mismatching of their dendrites to Or47b-expressing ORN axons.

(Hong et al., 2012)

Expression of Ten-a^GD8834 under the control of Scer\GAL4^Ten-m-NP6658 does not result in defects in muscle 3 innervation.

(Mosca et al., 2012)

Adults expressing Ten-a^GD8834 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

8834

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^peb-GAL4, Ten-a^GD8834 has abnormal neuroanatomy phenotype, enhanceable by Ten-m^GD3620/Scer\GAL4^peb-GAL4

(Hong et al., 2012)

Enhancer of

Statement

Reference

Scer\GAL4^peb-GAL4/Ten-a^GD8834 is an enhancer of abnormal neuroanatomy phenotype of Scer\GAL4^peb-GAL4, Ten-m^GD3620

(Hong et al., 2012)

Other

Statement

Reference

Scer\GAL4^elav-C155, Ten-a^GD8834, Ten-m^GD3620 has abnormal neuroanatomy phenotype

(Hong et al., 2012)

Phenotype Manifest In

Other

Statement

Reference

Scer\GAL4^Mef2.247, Ten-a^GD8834, Ten-m^GD3620 has cytoskeleton of presynaptic active zone phenotype

(Mosca et al., 2012)

Scer\GAL4^nrv2.PS, Ten-a^GD8834, Ten-m^GD3620 has cytoskeleton of presynaptic active zone phenotype

(Mosca et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of both Ten-a^GD8834 and Ten-m^GD3620 under the control of Scer\GAL4^elav-C155 shifts Or47b-expressing olfactory receptor neuron (ORN) axons to a position between two adjacent Ncra\QF^Mz19.PH-expressing glomeruli (DA1 and VA1d). In addition, Ncra\QF^Mz19.PH-expressing projection neuron (PN) dendrites and Or47b-expressing ORN axons intermingle without a clear border.

Co-expression of both Ten-a^GD8834 and Ten-m^GD3620 under the control of either Scer\GAL4^peb-GAL4 (expressed in the ORNs) or Scer\GAL4^GH146 (expressed in the PNs) results in intermingling of Ncra\QF^Mz19.PH-expressing PN dendrites and Or47b-expressing ORN axons.

(Hong et al., 2012)

Co-expression of Ten-m^GD3620 and Ten-a^GD8834 under the control of Scer\GAL4^Mef2.247 or Scer\GAL4^nrv2.PS results in defects in T-bar ultrastructure, membrane organisation and apposition to contractile tissues.

(Mosca et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v32482

w¹¹¹⁸; P{GD8834}v32482

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

8834

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Ten-a^GD8834

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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