FlyBase Allele Report: Dmel\ImpL2[UAS.cHa]

General Information

Symbol

Dmel\ImpL2^UAS.cHa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Honegger

FlyBase ID

FBal0249385

Feature type

allele

Associated gene

Dmel\ImpL2

Associated Insertion(s)

Carried in Construct

P{UAS-ImpL2.H}

Also Known As

UAS-ImpL2, UAS-Imp-L2

Key Links

Transgenic product class

wild_type

(Honegger et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Honegger et al., 2008)

Mutagen

in vitro construct

(Honegger et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-ImpL2.H}

Cytology

Description

UASt regulatory sequences drive expression of ImpL2.

(Honegger et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

ImpL2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate, with Scer\GAL4^ppl.PP

(Honegger et al., 2008)

abnormal stress response | female limited, with Scer\GAL4^hs.PB

(Alic et al., 2011)

abnormal stress response | female limited | RU486 conditional, with Scer\GAL4^{Act5C.Switch.PR}

(Alic et al., 2011)

decreased body size, with Scer\GAL4^arm.PS

(Honegger et al., 2008)

decreased body size, with Scer\GAL4^ppl.PP

(Honegger et al., 2008)

decreased cell size | somatic clone, with Scer\GAL4^GMR.PB

(Honegger et al., 2008)

decreased fecundity | female, with Scer\GAL4^hs.PB

(Alic et al., 2011)

decreased fecundity | female | RU486 conditional, with Scer\GAL4^{Act5C.Switch.PR}

(Alic et al., 2011)

lethal, with Scer\GAL4^Act5C.PI

(Honegger et al., 2008)

long lived | female limited, with Scer\GAL4^hs.PB

(Alic et al., 2011)

long lived | female limited, with Scer\GAL4^Ilp2.215-3

(Alic et al., 2011)

long lived | female limited | RU486 conditional, with Scer\GAL4^{Act5C.Switch.PR}

(Alic et al., 2011)

long lived | female limited | RU486 conditional, with Scer\GAL4^Switch1.106

(Alic et al., 2011)

viable, with Scer\GAL4^hs.PB

(Alic et al., 2011)

Phenotype Manifest In

adult neuromuscular junction, with Scer\GAL4^repo

(Jarabo et al., 2021)

eye, with Scer\GAL4^GMR.PF

(Honegger et al., 2008)

hemolymph, with Scer\GAL4^Myo31DF.PU, Scer\GAL80^ts.αTub84B

(Kwon et al., 2015)

ovary, with Scer\GAL4^Myo31DF.PU, Scer\GAL80^ts.αTub84B

(Kwon et al., 2015)

presynaptic active zone | adult stage | decreased number, with Scer\GAL4^repo

(Jarabo et al., 2021)

Detailed Description

Statement

Reference

Adults expressing ImpL2^UAS.cHa under the control of Scer\GAL4^repo show neuromuscular junctions with fewer active zones.

(Jarabo et al., 2021)

Expression of ImpL2^Scer\UAS.cHa in adult enterocytes (under the control of Scer\GAL4^Myo31DF.PU and limited to the adult stage using Scer\GAL80^ts.αTub84B) leads to increased circulating and whole-body trehalose levels, increased hemolymph volume and ovary atrophy.

(Kwon et al., 2015)

Overexpression of ImpL2^Scer\UAS.cHa via Scer\GAL4^hs.PB results in viable flies.

Overexpression of ImpL2^Scer\UAS.cHa via Scer\GAL4^hs.PB increases female median lifespan, oxidative stress resistance and whole-fly lipid content, while reducing fecundity.

Overexpression of ImpL2^Scer\UAS.cHa via RU486-induced Scer\GAL4^{Act5C.Switch.PR} increases both median and maximal lifespan of female flies.

Overexpression of ImpL2^Scer\UAS.cHa via RU486-induced Scer\GAL4^{Act5C.Switch.PR} increases oxidative stress resistance of female flies, while reducing fecundity.

Overexpression of ImpL2^Scer\UAS.cHa with the pan-neuronal driver Scer\GAL4^elav.PLu does not extend lifespan.

Overexpression of ImpL2^Scer\UAS.cHa with Scer\GAL4^Akh.PG does not extend lifespan.

Overexpression of ImpL2^Scer\UAS.cHa via Scer\GAL4^Ilp2.215-3 significantly extends both median and maximal lifespan of female flies.

Overexpression of ImpL2^Scer\UAS.cHa via RU486-induced Scer\GAL4^Switch1.106 significantly extends both median and maximal lifespan of female flies.

(Alic et al., 2011)

Ubiquitous overexpression of ImpL2^Scer\UAS.cHa under the control of Scer\GAL4^Act5C.PI leads to lethality.

Ubiquitous overexpression of ImpL2^Scer\UAS.cHa under the control of Scer\GAL4^arm.PS results in flies that are decreased in size and weight.

Overexpression of ImpL2^Scer\UAS.cHa under the control of Scer\GAL4^arm.PS in somatic clones results in a non-autonomous reduction of cell size.

Overexpression of ImpL2^Scer\UAS.cHa driven by Scer\GAL4^GMR.PF results in a strong reduction of eye size. These ImpL2^Scer\UAS.cHa-overexpressing flies are of normal size.

Flies overexpressing ImpL2^Scer\UAS.cHa under the control of Scer\GAL4^ppl.PP display a pronounced reduction in body size and their development is delayed by two days.

(Honegger et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

ImpL2^UAS.cHa/Scer\GAL4^Act5C.PP is an enhancer of increased cell growth rate phenotype of Pten¹¹⁷

(Nowak et al., 2013)

ImpL2^UAS.cHa/Scer\GAL4^Act5C.PP is an enhancer of increased body size phenotype of Pten¹¹⁷

(Nowak et al., 2013)

ImpL2^UAS.cHa/Scer\GAL4^Act5C.PP is an enhancer of increased cell growth rate | somatic clone phenotype of Pten¹¹⁷

(Nowak et al., 2013)

ImpL2^UAS.cHa/Scer\GAL4^Act5C.PP is an enhancer of increased cell growth rate | somatic clone phenotype of Pten¹¹⁷, mTor^2L19

(Nowak et al., 2013)

Suppressor of

Statement

Reference

ImpL2^UAS.cHa, Scer\GAL4^GMR.PF is a suppressor | partially of visible phenotype of InR^UAS.cHa, Scer\GAL4^GMR.PF

(Honegger et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

ImpL2^UAS.cHa, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Df(3L)AC1/+

(Honegger et al., 2008)

NOT suppressed by

Statement

Reference

ImpL2^UAS.cHa, Scer\GAL4^Myo31DF.PU, Scer\GAL80^ts.αTub84B has hemolymph phenotype, non-suppressible by InR^act.UAS, Scer\GAL4^Myo31DF.PU

(Kwon et al., 2015)

ImpL2^UAS.cHa, Scer\GAL4^Myo31DF.PU, Scer\GAL80^ts.αTub84B has ovary phenotype, non-suppressible by InR^act.UAS, Scer\GAL4^Myo31DF.PU

(Kwon et al., 2015)

Suppressor of

Statement

Reference

ImpL2^UAS.cHa, Scer\GAL4^GMR.PF is a suppressor | partially of eye phenotype of InR^UAS.cHa, Scer\GAL4^GMR.PF

(Honegger et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of InR^act.Scer\UAS does not suppress the increases in circulating and whole-body trehalose levels and hemolymph volume and ovary atrophy seen when ImpL2^Scer\UAS.cHa is expressed in adult enterocytes (under the control of Scer\GAL4^Myo31DF.PU and limited to the adult stage using Scer\GAL80^ts.αTub84B).

(Kwon et al., 2015)

Ubiquitous expression of ImpL2^Scer\UAS.cHa (under the control of Scer\GAL4^Act5C.PP) results in the overgrowth of Pten¹¹⁷ clones, irrespective of whether they neighbor Tor^2L19 mutant or wild-type cells. The overgrowth of Pten¹¹⁷ clones causes a further reduction in body size, as evidenced by decreased shoulder width.

(Nowak et al., 2013)

ImpL2^Scer\UAS.cHa partially suppresses the eye overgrowth resulting from the overexpression of InR^Scer\UAS.cHa driven by Scer\GAL4^GMR.PF.

Df(3L)AC1 dominantly enhances the small eye phenotype caused by Scer\GAL4^GMR.PF-driven overexpression of ImpL2^Scer\UAS.cHa.

(Honegger et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ImpL2^Scer\UAS.cHa

ImpL2^UAS.cHa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Honegger

Secondary FlyBase IDs

References (9)

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