FlyBase Gene Report: Dmel\lap

General Information

Symbol

Dmel\lap

Species

D. melanogaster

Name

like-AP180

Annotation Symbol

CG2520

Feature Type

protein_coding_gene

FlyBase ID

FBgn0086372

Gene Model Status

Current

Stock Availability

15 publicly available

Gene Summary

like-AP180 (lap) encodes a cytosolic protein that acts as a clathrin adaptor to promote clathrin-coated vesicle formation and restrict coated vesicle size. At synapses it regulates synaptic vesicle size as well as the efficacy of synaptic vesicle protein retrieval. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
UniProtKB
Interactive Fly

Also Known As

AP180, l(3)neo34, dAP-3, EP3060

Key Links

Genomic Location

Cytogenetic map

84D1-84D2

Sequence location

3R:7,186,994..7,200,941 [+]

Recombination map

3-48

RefSeq locus

NT_033777 REGION:7186994..7200941

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (19 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

enables 1-phosphatidylinositol binding

inferred from sequence or structural similarity with UniProtKB:O55012

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN000536700

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR014712

(InterPro Project Members, 2004-)

enables clathrin binding

inferred from sequence or structural similarity with UniProtKB:Q13492

(Gene Ontology Curators, 2002-)

inferred from electronic annotation with InterPro:IPR014712

(InterPro Project Members, 2004-)

enables clathrin heavy chain binding

inferred from biological aspect of ancestor with PANTHER:PTN000536700

(GO Reference Genome Project, 2011-)

enables phosphatidylinositol-4,5-bisphosphate binding

inferred from biological aspect of ancestor with PANTHER:PTN000536700

(GO Reference Genome Project, 2011-)

enables SNARE binding

inferred from biological aspect of ancestor with PANTHER:PTN000536700

(GO Reference Genome Project, 2011-)

Biological Process (10 terms)

Terms Based on Experimental Evidence (7 terms)

CV Term

Evidence

References

involved_in chemical synaptic transmission

inferred from mutant phenotype

(Karunanithi et al., 2002)

involved_in clathrin-dependent synaptic vesicle endocytosis

inferred from mutant phenotype

(Zhang et al., 1998)

involved_in neuron-neuron synaptic transmission

inferred from mutant phenotype

(Babcock et al., 2003)

involved_in phototaxis

inferred from mutant phenotype

(Babcock et al., 2003)

involved_in positive regulation of clathrin-dependent endocytosis

inferred from mutant phenotype

(Vanlandingham et al., 2014)

involved_in receptor-mediated endocytosis

inferred from direct assay

(Zhang et al., 1998)

involved_in synaptic vesicle transport

inferred from mutant phenotype

(Vanlandingham et al., 2014)

inferred from physical interaction with FLYBASE:Csp; FB:FBgn0004179

(Vanlandingham et al., 2014)

inferred from physical interaction with FLYBASE:Syt1; FB:FBgn0004242

(Vanlandingham et al., 2014)

inferred from physical interaction with FLYBASE:nSyb; FB:FBgn0013342

(Vanlandingham et al., 2014)

inferred from physical interaction with FLYBASE:VGlut; FB:FBgn0031424

(Vanlandingham et al., 2014)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in clathrin coat assembly

inferred from electronic annotation with InterPro:IPR014712, InterPro:IPR045192

(InterPro Project Members, 2004-)

involved_in clathrin-dependent endocytosis

inferred from electronic annotation with InterPro:IPR045192

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000536700

(GO Reference Genome Project, 2011-)

involved_in synaptic vesicle budding from presynaptic endocytic zone membrane

inferred from biological aspect of ancestor with PANTHER:PTN001119474

(GO Reference Genome Project, 2011-)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in clathrin-coated pit

inferred from direct assay

(Zhang et al., 1998)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

is_active_in clathrin-coated pit

inferred from biological aspect of ancestor with PANTHER:PTN000536700

(GO Reference Genome Project, 2011-)

is_active_in clathrin-coated vesicle

inferred from biological aspect of ancestor with PANTHER:PTN000536700

(GO Reference Genome Project, 2011-)

located_in clathrin-coated vesicle

inferred from electronic annotation with InterPro:IPR014712

(InterPro Project Members, 2004-)

is_active_in extrinsic component of presynaptic endocytic zone membrane

inferred from biological aspect of ancestor with PANTHER:PTN001119474

(GO Reference Genome Project, 2011-)

is_active_in synaptic vesicle

inferred from biological aspect of ancestor with PANTHER:PTN001119474

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

Protein Family (UniProt)

Belongs to the PICALM/SNAP91 family. (Q9VI75)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Protein Function (UniProtKB)

Assembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction.

(UniProt, Q9VI75)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\lap for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VI75)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

1HX8

Comments on Gene Model

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.47

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Multiphase exon postulated: exon reading frame differs in alternative transcripts; overlap >20aa.

Sequence Ontology: Class of Gene

gene

gene_with_edited_transcript

(Rodriguez et al., 2012, Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

lap-RA

FBtr0081572

NM_079528

4341

468

lap-RC

FBtr0302183

NM_001144547

5126

758

lap-RD

FBtr0302184

NM_001170067

5216

788

lap-RE

FBtr0305153

NM_001260029

2984

572

lap-RF

FBtr0305154

NM_001260030

3249

685

lap-RG

FBtr0305155

NM_001260031

4871

673

lap-RH

FBtr0334886

NM_001275409

4964

704

lap-RI

FBtr0334887

NM_001275410

5180

776

lap-RJ

FBtr0334888

NM_001275411

4886

678

Additional Transcript Data and Comments

Reported size (kB)

4.2 (northern blot)

(Zhang et al., 1998)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

lap-PA

FBpp0081091

49.9

468

6.12

FBpp0291393

80.9

758

7.20

FBpp0291394

84.2

788

7.28

FBpp0293683

61.7

572

6.68

FBpp0293684

72.8

685

7.46

FBpp0293685

71.8

673

7.70

FBpp0306907

75.0

704

7.93

FBpp0306908

83.2

776

7.49

FBpp0306909

72.4

678

8.15

A0A0B4KF90

NP_001262340

AGB95723

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

468 (aa)

(Zhang et al., 1998)

Comments

External Data

Subunit Structure (UniProtKB)

Binds clathrin and phosphatidylinositol 4,5-bisphosphate.

(UniProt, Q9VI75)

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

1HX8

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\lap using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.17

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

garland cell

(Zhang et al., 1998)

presumptive embryonic/larval peripheral nervous system

(Zhang et al., 1998)

presumptive embryonic/larval central nervous system

(Zhang et al., 1998)

Additional Descriptive Data

lap transcripts are expressed mainly in the central and peripheral nervous systems and in garland cells in embryos.

(Zhang et al., 1998)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

bouton

(Zhang et al., 1998)

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

neuromuscular junction

(Zhang et al., 1998)

neuromuscular junction | restricted

Comment: Presynaptic

(Verstreken et al., 2009)

third instar larval stage

embryonic/larval neuromuscular junction

• terminal bouton

(Bronk et al., 2001)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

lap protein is enriched at synaptic boutons of neuromuscular junctions in larvae. lap protein and Clc protein co-localize at the synaptic boutons. The level of Clc protein is significantly reduced in lap mutants.

(Zhang et al., 1998)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in clathrin-coated pit

inferred from direct assay

(Zhang et al., 1998)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\lap in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\lap

View exonic expression by tissue for Dmel\lap

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40863/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0086372

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0086372

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0086372

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2520

Flygut - An atlas of the Drosophila adult midgut

FBgn0086372

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 15 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 12 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of lap

Transgenic constructs containing regulatory region of lap

Aberrations (Deficiencies and Duplications) ( 7 )

Inferred from experimentation ( 7 )

Gene disrupted in

Df(3R)Antp1

(Babcock et al., 2003, Zhang et al., 1998)

Df(3R)Antp17

(Babcock et al., 2003, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Df(3R)BSC221

(Gresens and Cook, 2006.12.5)

Df(3R)dsx2M

(Babcock et al., 2003)

Df(3R)Exel6146

(Ryder, 2004.4.26)

Df(3R)Scx4

(Babcock et al., 2003)

Gene not disrupted in

Df(3R)BSC548

(Christensen et al., 2008.6.11)

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal (with lap^KG06751)

lap^sd-3

lethal (with lap^sd-3)

lap^KG06751

lethal | larval stage | recessive

lap^sd-3

lethal | pupal stage | recessive

lap^sd-3

lethal | recessive

lap¹

lethal - all die before end of larval stage (with lap¹)

lap^sd-3

lethal - all die before end of larval stage (with lap^sd-3)

lap¹

lethal - all die before end of larval stage | recessive

lap¹

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

lap^GD4725

partially lethal - majority die | recessive

lap¹

viable

viable, with Scer\GAL4^Mef2.PR

lap^GD4725

viable, with Scer\GAL4^pnr-MD237

lap^GD4725

viable, with Scer\GAL4^tin.CΔ4

lap^GD4725

Sterility

Allele

fertile

lap^EY11719

Other Phenotypes

Allele

abnormal locomotor behavior | recessive

lap¹

abnormal neuroanatomy

lap¹

abnormal neuroanatomy | conditional, with lap^sd-3/lap¹

lap^{C.4C.Tag:FLAG,Tag:CALI(TC)}

abnormal neuroanatomy | conditional (with lap¹), with lap^{C.4C.Tag:FLAG,Tag:CALI(TC)}

lap^sd-3

abnormal neuroanatomy | conditional (with lap^sd-3), with lap^{C.4C.Tag:FLAG,Tag:CALI(TC)}

lap¹

abnormal neuroanatomy | third instar larval stage

lap¹

abnormal neurophysiology (with lap¹)

lap^sd-3

abnormal neurophysiology (with lap^sd-3)

lap¹

abnormal neurophysiology | larval stage | recessive

lap¹

abnormal neurophysiology | somatic clone

lap^sd-3

abnormal neurophysiology | third instar larval stage

lap¹

abnormal phototaxis | somatic clone

lap^sd-3

decreased rate of movement | recessive

lap¹

long lived | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

lap^HMS01939

short lived | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

lap^A.UAS

some die during larval stage | recessive

lap¹

some die during pupal stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

lap^GD4725

uncoordinated | larval stage | recessive

lap¹

visible, with Scer\GAL4^GMR.PF

lap^EP3060

Phenotype manifest in

Allele

embryonic/larval neuromuscular junction | third instar larval stage | conditional, with lap^sd-3/lap¹

lap^{C.4C.Tag:FLAG,Tag:CALI(TC)}

embryonic/larval neuromuscular junction | third instar larval stage | conditional (with lap¹), with lap^{C.4C.Tag:FLAG,Tag:CALI(TC)}

lap^sd-3

embryonic/larval neuromuscular junction | third instar larval stage | conditional (with lap^sd-3), with lap^{C.4C.Tag:FLAG,Tag:CALI(TC)}

lap¹

eye, with Scer\GAL4^GMR.PF

lap^EP3060

NMJ bouton | increased number

lap¹

NMJ bouton | third instar larval stage | conditional

lap^{C.4C.Tag:FLAG,Tag:CALI(TC)}

ommatidium, with Scer\GAL4^GMR.PF

lap^EP3060

synaptic vesicle

lap¹

type I bouton | third instar larval stage

lap¹

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (2)

Hsap\PICALM

13 of 14

Yes

Hsap\SNAP91

11 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (2)

Rnor\Picalm

13 of 14

Yes

Rnor\Snap91

11 of 14

Yes

Mus musculus (laboratory mouse) (3)

Mmus\Picalm

12 of 14

Yes

Mmus\Snap91

11 of 14

Yes

Mmus\Zan

1 of 14

Xenopus tropicalis (Western clawed frog) (3)

Xtro\snap91

10 of 13

Yes

Xtro\picalm

8 of 13

Yes

Xtro\picalml

1 of 13

Yes

Danio rerio (Zebrafish) (6)

Drer\picalma

13 of 14

Yes

Drer\picalmb

11 of 14

Yes

Drer\si:ch211-200p22.4

11 of 14

Yes

Drer\snap91b

11 of 14

Yes

Drer\snap91a

8 of 14

Yes

Drer\wu:fj42b12

3 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\unc-11

11 of 14

Yes

Anopheles gambiae (African malaria mosquito) (1)

Agam\AgaP_AGAP010087

12 of 12

Yes

Arabidopsis thaliana (thale-cress) (18)

Atha\AT1G03050

8 of 13

Yes

Atha\AT1G14910

8 of 13

Yes

Atha\AT4G02650

8 of 13

Yes

Atha\AT5G35200

8 of 13

Yes

Atha\AT5G57200

8 of 13

Yes

Atha\AT2G01600

7 of 13

Yes

Atha\AT4G25940

7 of 13

Yes

Atha\AT1G05020

6 of 13

Yes

Atha\AT4G32285

6 of 13

Yes

Atha\AT2G25430

5 of 13

Yes

Atha\AT1G14686

3 of 13

Yes

Atha\AT1G25240

3 of 13

Yes

Atha\AT1G33340

3 of 13

Yes

Atha\AT1G68110

3 of 13

Yes

Atha\AT2G01920

3 of 13

Yes

Atha\AT4G40080

3 of 13

Yes

Atha\AT5G10410

2 of 13

Yes

Atha\AT5G65370

2 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\YAP1801

11 of 13

Yes

Scer\YAP1802

10 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\SPBC19F8.03c

9 of 12

Yes

Spom\end4

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:lap. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (2)

CG14190

1 of 13

dpy

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Alzheimer disease, susceptibility to, PICALM-related

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 3 )

Allele

Disease

Evidence

References

lap¹

model of Alzheimer's disease

CEA with Hsap\MAPT^4R.UAS

(Moreau et al., 2014)

lap^GD4725

model of Alzheimer's disease

CEA with Hsap\MAPT^4R.UAS

(Moreau et al., 2014)

lap^KK109279

model of Alzheimer's disease

CEA with Hsap\MAPT^4R.UAS

(Moreau et al., 2014)

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

PICALM; phosphatidylinositol binding clathrin assembly protein

model of acute myeloid leukemia

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

lap^HMS01939

exacerbates Alzheimer's disease 1

modeled by Hsap\APP^2xAPP.SP.UAS

(Yu et al., 2020)

lap^A.UAS

ameliorates Alzheimer's disease 1

modeled by Hsap\APP^2xAPP.SP.UAS

(Yu et al., 2020)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PICALM; phosphatidylinositol binding clathrin assembly protein

13 of 14

603025

LEUKEMIA, ACUTE MYELOID; AML

acute myeloid leukemia

SNAP91; synaptosome associated protein 91

11 of 14

607923

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

lap - Csp

anti tag coimmunoprecipitation, western blot

(Vanlandingham et al., 2014)

lap - Syt1

anti tag coimmunoprecipitation, western blot

(Vanlandingham et al., 2014)

lap - VGlut

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation

(Yu et al., 2020, Vanlandingham et al., 2014)

lap - nSyb

anti tag coimmunoprecipitation, western blot

(Vanlandingham et al., 2014)

RNA-protein

Physical Interaction

Assay

References

lap - how

pull down, anti tag western blot

(Toledano-Katchalski et al., 2007)

Summary of Genetic Interactions

esyN Network Diagram

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

esyN Network Key:

Suppression

Enhancement

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Sun et al., 2006)

enhanceable

(Sun et al., 2006)

enhanceable

(Sun et al., 2006)

enhanceable

(Sun et al., 2006)

enhanceable

(Sun et al., 2006)

enhanceable

(Sun et al., 2006)

enhanceable

(Sun et al., 2006)

enhanceable

(Sun et al., 2006)

enhanceable

(Sun et al., 2006)

enhanceable

(Sun et al., 2006)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

lap

(Müller et al., 2005)

ND-42

suppressible

lap

(Moulton et al., 2021)

pnr

enhanceable

lap

(Pena-Rangel et al., 2002)

External Data

Subunit Structure (UniProtKB)

Binds clathrin and phosphatidylinositol 4,5-bisphosphate.

(UniProt, Q9VI75 )

Linkouts

BioGRID - A database of protein and genetic interactions.

66058

DroID - A comprehensive database of gene and protein interactions.

FBgn0086372

MIST (protein-protein) - An integrated Molecular Interaction Database

40863

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-48

Cytogenetic map

84D1-84D2

Sequence location

3R:7,186,994..7,200,941 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

84D1-84D2

Limits computationally determined from genome sequence between P{EP}EP3060^EP3060 and P{PZ}grn⁰⁵⁹³⁰

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Determined by comparing Celera genomic sequence with sequence from BDGP BAC and P1 clones.

(Lloyd et al., 2000)

84C-84D

(Lloyd et al., 2000, Zhang, 1998.6.30)

84D1-84D14

(determined by in situ hybridisation)

(Spradling et al., 1999)

84C-84D

(determined by in situ hybridisation)

(Zhang et al., 1998)

84D1-84D14

(BDGP Project Members, 1994-1999)

84D-84D

(determined by in situ hybridisation)

(Cooley et al., 1988)

Experimentally Determined Recombination Data

Location

3-48

(FlyBase, 2016)

3-44.2

(Comeron et al., 2012)

3-45.6 +/- 10.4

(Babcock et al., 2003)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (15)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

26463

w¹¹¹⁸; Mi{ET1}lap^MB09294

28358

y¹ v¹; P{TRiP.JF02994}attP2

93752

w^*; lap^KO/TM3, Sb¹

55241

y¹ v¹; P{TRiP.HMC02373}attP2

39021

y¹ v¹; P{TRiP.HMS01939}attP40

92510

y¹ v¹; P{U6.2-lap.dgRNA}attP40/CyO

76532

y¹ sc^* v¹ sev²¹; P{TKO.GS00983}attP40

77303

y¹ sc^* v¹ sev²¹; P{TOE.GS01980}attP40

Kyoto

104345

y^* w^*; P{GawB}lap^NP3025 / TM6, P{UAS-lacZ.UW23-1}UW23-1

NIG-Fly

HMS01939

y¹ v¹; P{TRiP.HMS01939}attP40

VDRC

v105767

P{KK109279}VIE-260B

v12732

w¹¹¹⁸; P{GD4725}v12732

More stocks available...

Genomic Clones (19)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (63)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RH47395

Other clones

MIP05850

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0086372

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0086372

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

A mutation in lap severely impairs the efficiency of synaptic vesicle endocytosis.

(Zhang et al., 1998)

Relationship to Other Genes

Source for database merge of

Source for merge of: lap EP3060

(Sun et al., 2006)

Source for merge of: lap BcDNA:GM10787

(FlyBase, 1996-)

Additional comments

FlyBase curator comment: "EP3060" overexpression phenotype stated to be due to its effect on EST RE52393 (which corresponds to CG10055) but orientation of the P{EP} element suggests otherwise.

(Pena-Rangel et al., 2002)

Source for merge of lap BcDNA:GM10787 was a shared cDNA ( date:020730 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: lap CG2520

(Milshina, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (17)

Reported As

Symbol Synonym

AP180

(Sauvola and Littleton, 2021, Latcheva et al., 2019, Vanlandingham et al., 2014, Verstreken et al., 2009, Korolchuk et al., 2007, Pack-Chung et al., 2007, Dickman et al., 2006, Verstreken and Bellen, 2006, Broadie, 2004, Marie et al., 2004, Narayanan and Ramaswami, 2001, Zhang et al., 1998)

BcDNA:GM10787

CG2520

(Trivedi et al., 2020, Çiçek et al., 2016, Balakrishnan et al., 2015, Cammarato et al., 2011, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Stuart et al., 2007, Toledano-Katchalski et al., 2007, Casillas et al., 2006, Provost et al., 2006, Robertson, 2001.7.7, Verstreken and Bellen, 2000.9.4)

EP(3)3060

(Pena-Rangel et al., 2002)

EP3060

LAP

(Sauvola and Littleton, 2021, Bao et al., 2007, Pack-Chung et al., 2007, Bronk et al., 2001, Lloyd et al., 2000, McMahon, 1999, Spradling et al., 1999)

Lap

(van de Leemput et al., 2022, Denton et al., 2020, Dourlen et al., 2018, Korolchuk et al., 2007, Majumdar et al., 2006, Chen et al., 2002)

dAP-3

(Zhang and Ganetzky, 1997, Zhang and Ganetzky, 1997)

l(3)neo34

(Provost et al., 2006, Cooley et al., 1988)

lap

(Lee et al., 2023, Peng et al., 2021, Uçkun et al., 2021, Wang et al., 2021, Yu et al., 2020, Zhao et al., 2020, Gene Disruption Project members, 2018-, Sanfilippo et al., 2017, Transgenic RNAi Project members, 2017-, Ashwal-Fluss et al., 2014, Gupta et al., 2014, Moreau et al., 2014, Vanlandingham et al., 2014, Kwon et al., 2013, Terriente-Felix et al., 2013, Izutsu et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Matta et al., 2012, Rodriguez et al., 2012, Savva et al., 2012, Bhogal et al., 2011, Guan et al., 2011, Kopyl et al., 2010, Kopyl et al., 2010, Tsurudome et al., 2010, Wasbrough et al., 2010, Dickman and Davis, 2009, Jepson and Reenan, 2009, Christensen et al., 2008.4.15, Christensen et al., 2008.6.11, FlyBase Curators, 2008-, Kaplow et al., 2007, Stuart et al., 2007, Toledano-Katchalski et al., 2007, Dickman, 2006, Gresens and Cook, 2006.12.5, Provost et al., 2006, Sun et al., 2006, Bao et al., 2005, Liebl and Featherstone, 2005, Cherry and Perrimon, 2004)

lap/AP180

(Koh et al., 2004)

sig

(Gruber and Buening, 1999)

Name Synonyms

EP3060

Lap

(Koehler and Huber, 2023)

Like-AP180

(Bao et al., 2005)

complementation group 12

(Babcock et al., 2003)

like AP180

(Cherry and Perrimon, 2004)

like-AP180

(Gupta et al., 2014, Cammarato et al., 2011, Kaplow et al., 2007, Zhang, 1998.6.30, Zhang et al., 1998)

saisoog

(Gruber and Buening, 1999)

Secondary FlyBase IDs

FBgn0026210
FBgn0062670
FBgn0011467
FBgn0026062
FBgn0047251

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 82 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

40863

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VI75

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VI75

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VI75

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40863/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0086372

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0086372

FlyMine - An integrated database for Drosophila genomics

FBgn0086372

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG2520

MARRVEL_MODEL - MARRVEL (model organism gene)

40863

PDB - An information portal to biological macromolecular structures

1HX8

Linkouts

BioGRID - A database of protein and genetic interactions.

66058

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0086372

DroID - A comprehensive database of gene and protein interactions.

FBgn0086372

DRSC - Results frm RNAi screens

FBgn0086372

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0086372

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0086372

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2520

Flygut - An atlas of the Drosophila adult midgut

FBgn0086372

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0086372

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

MIST (protein-protein) - An integrated Molecular Interaction Database

40863

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (127)

The following external sites may use different assemblies or annotations than FlyBase.

-1.17

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