FB2024_03 , released June 25, 2024
Allele: Dmel\pntP1.UAS
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General Information
Symbol
Dmel\pntP1.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0035443
Feature type
allele
Associated gene
Dmel\pnt
Associated Insertion(s)
Carried in Construct
P{UAS-pnt.P1}
Also Known As
UAS-PNTP1, UAS-pnt.P1, UAS-pntP1, UAS-pnt-P1
Key Links
Transgenic product class
wild_type
(Klaes et al., 1994)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Klaes et al., 1994)
Mutagen
in vitro construct
(Albagli et al., 1996, Klaes et al., 1994)
Progenitor genotype
Carried in construct
P{UAS-pnt.P1}
Cytology
Description

UASt regulatory sequences drive expression of the pnt P1 isoform (the entire open reading frame plus 500bp of 5'UTR and 260bp of 3'UTR sequence).

(Klaes et al., 1994)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
pnt
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  carcinoma
      modeled by Ras85DV12.UAS
      (Ito and Igaki, 2021)
      modeled by Ras85DV12.UAS, l(2)gl4
      (Ito and Igaki, 2021)
      modeled by Ras85DV12.UAS, dlg114
      (Ito and Igaki, 2021)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class

      abnormal size, with Scer\GAL4ey.PU

      (Salzer et al., 2010)

      decreased occurrence of cell division | larval stage | somatic clone, with Scer\GAL4Act.PU

      (Ito and Igaki, 2021)

      decreased size | larval stage | somatic clone, with Scer\GAL4Act.PU

      (Ito and Igaki, 2021)

      female semi-sterile, with Scer\GAL455B

      (Morimoto et al., 1996)

      female semi-sterile, with Scer\GAL4c324a

      (Morimoto et al., 1996)

      female sterile, with Scer\GAL455B

      (Morimoto et al., 1996)

      female sterile, with Scer\GAL4c324a

      (Morimoto et al., 1996)

      increased cell number | embryonic stage 15, with Scer\GAL4prd.PU

      (Wang et al., 2017)

      increased cell number | embryonic stage 15, with Scer\GAL4salm-459.2

      (Wang et al., 2017)

      increased cell number | larval stage, with Scer\GAL4He.PZ

      (Zettervall et al., 2004)

      increased cell number | larval stage, with Scer\GAL4Hml.PG

      (Zettervall et al., 2004)

      increased cell size | larval stage | somatic clone, with Scer\GAL4Act.PU

      (Ito and Igaki, 2021)

      increased occurrence of cell division | adult stage, with Scer\GAL4Delta.PU

      (Jin et al., 2015)

      increased occurrence of cell division | adult stage, with Scer\GAL4NP5130

      (Jin et al., 2015)

      lethal - all die during embryonic stage, with Scer\GAL4nanos.PG

      (Tracey et al., 2000)

      short lived, with Scer\GAL4Switch1.106

      (Alic et al., 2014)

      short lived, with Scer\GAL4TIGS-2

      (Alic et al., 2014)

      short lived | RU486 conditional, with Scer\GAL4da.Switch.PT

      (Slack et al., 2015)

      some die during embryonic stage, with Scer\GAL4nanos.PG

      (Tracey et al., 2000)

      visible, with Scer\GAL471B

      (Wessells et al., 1999)

      visible, with Scer\GAL4dpp.blk1

      (Wessells et al., 1999)

      visible, with Scer\GAL4ey.PU

      (Salzer et al., 2010)

      visible | homeotic, with Scer\GAL4ey.PB

      (Kumar and Moses, 2001)
      Phenotype Manifest In

      adult midgut, with Scer\GAL4Delta.PU

      (Jin et al., 2015)

      adult midgut, with Scer\GAL4NP5130

      (Jin et al., 2015)

      antenna | ectopic, with Scer\GAL4ey.PB

      (Kumar and Moses, 2001)

      cardioblast, with Scer\GAL4twi.PG

      (Alvarez et al., 2003)

      central nervous system, with Scer\GAL4rho.PL

      (Klaes et al., 1994)

      central nervous system, with Scer\GAL4sca-537.4

      (Klaes et al., 1994)

      cephalopharyngeal skeleton, with Scer\GAL4nanos.PG

      (Tracey et al., 2000)

      dorsal appendage, with Scer\GAL455B

      (Morimoto et al., 1996)

      dorsal appendage, with Scer\GAL4c324a

      (Morimoto et al., 1996)

      embryonic/larval oenocyte | ectopic | embryonic stage 15, with Scer\GAL4prd.PU

      (Wang et al., 2017)

      embryonic/larval oenocyte | embryonic stage 15, with Scer\GAL4prd.PU

      (Wang et al., 2017)

      embryonic/larval oenocyte | embryonic stage 15, with Scer\GAL4salm-459.2

      (Wang et al., 2017)

      embryonic/larval tracheal section, with Scer\GAL4btl.PS

      (Mortimer and Moberg, 2009)

      embryonic longitudinal glial cell | ectopic, with Scer\GAL4sca-537.4

      (Klaes et al., 1994)

      embryonic pericardial cell, with Scer\GAL4twi.PG

      (Alvarez et al., 2003)

      eye, with Scer\GAL4ey.PB

      (Kumar and Moses, 2001)

      eye, with Scer\GAL4ey.PU

      (Salzer et al., 2010)

      eye, with Scer\GAL4hs.2sev

      (Albagli et al., 1996)

      eye-antennal disc, with Scer\GAL4Act.PU

      (Ito and Igaki, 2021)

      eye disc morphogenetic furrow | ectopic, with Scer\GAL4dpp.blk1

      (Kumar and Moses, 2001)

      eye photoreceptor cell, with Scer\GAL4sca-T3

      (Frankfort and Mardon, 2004)

      hemocyte, with Scer\GAL4He.PZ

      (Zettervall et al., 2004)

      hemocyte, with Scer\GAL4Hml.PG

      (Zettervall et al., 2004)

      intestinal stem cell | adult stage, with Scer\GAL4Delta.PU

      (Jin et al., 2015)

      intestinal stem cell | adult stage, with Scer\GAL4NP5130

      (Jin et al., 2015)

      macrochaeta | ectopic, with Scer\GAL4dpp.blk1

      (Wessells et al., 1999)

      melanotic mass, with Scer\GAL4He.PZ

      (Zettervall et al., 2004)

      melanotic mass, with Scer\GAL4Hml.PG

      (Zettervall et al., 2004)

      midline glial cell, with Scer\GAL4sim.PS

      (Scholz et al., 1997, Scholz et al., 1997)

      muscle founder cell | increased number, with Scer\GAL41151

      (Dutta et al., 2005)

      ommatidium, with Scer\GAL4hs.2sev

      (Albagli et al., 1996)

      ommatidium, with Scer\GAL4sca-T3

      (Frankfort and Mardon, 2004)

      photoreceptor cell R8, with Scer\GAL4sca-T3

      (Frankfort and Mardon, 2004)

      pigment cell, with Scer\GAL4hs.2sev

      (Albagli et al., 1996)

      rhabdomere R8, with Scer\GAL4sca-T3

      (Frankfort and Mardon, 2004)

      submarginal cell, with Scer\GAL471B

      (Wessells et al., 1999)

      symmetrical commissure, with Scer\GAL4rho.PL

      (Klaes et al., 1994)

      symmetrical commissure, with Scer\GAL4sca-537.4

      (Klaes et al., 1994)

      wing, with Scer\GAL471B

      (Wessells et al., 1999)

      wing, with Scer\GAL4dpp.blk1

      (Wessells et al., 1999)

      wing disc, with Scer\GAL4dpp.blk1

      (Wessells et al., 1999)

      wing vein L4, with Scer\GAL471B

      (Wessells et al., 1999)
      Detailed Description
      Statement
      Reference

      Clonal expression of pntP1.UAS under the control of Scer\GAL4Act.PU leads to smaller clones than controls, with an associated cell cycle arrest in G1 and increased cell sizes.

      (Ito and Igaki, 2021)

      The expression of pntP1.Scer\UAS under the control of Scer\GAL4prd.PU leads to extra and ectopic oenocytes in Prd-positive abdominal and thoracic segments, respectively, during stage 15 of embryogenesis, as compared to controls; expression under the control of Scer\GAL4salm-459.2, but not Scer\GAL4ato.3.6, also leads to extra oenocytes in abdominal segments, as compared to controls.

      (Wang et al., 2017)

      Expression of pntP1.Scer\UAS under the control of either Scer\GAL4esg-NP5130 or Scer\GAL4Dl.PU (using tub-Gal80[ts] to limit the time of the transgene expression) results in intestinal stem cell overproliferation in the adult midgut.

      (Jin et al., 2015)

      The short lived phenotype seen in flies expressing pntP1.Scer\UAS under the control of Scer\GAL4da.Switch.PT is responsive to dietary yeast.

      trametinib treatment at 15.6uM has no effect on the shortened lifespan seen in flies expressing Ras85DV12.cUa.Scer\UAS under the control of Scer\GAL4da.Switch.PT.

      (Slack et al., 2015)

      Expression of pntP1.Scer\UAS in the adult gut and fat body under the control of Scer\GAL4Switch1.106 (in the presence of RU486) has a marginal negative effect on lifespan compared to controls.

      Expression of pntP1.Scer\UAS in the adult gut under the control of Scer\GAL4TIGS-2 (in the presence of RU486) has detrimental effect on lifespan compared to controls. Stored triacylglycerol (TAG) levels are also reduced compared to controls. Gut integrity and feeding appear normal.

      (Alic et al., 2014)

      Expression of pntP1.Scer\UAS driven by Scer\GAL4ey.PU causes a dramatic reduction in the size of the adult eye.

      (Salzer et al., 2010)

      Scer\GAL4btl.PS-mediated expression of pntP1.Scer\UAS disrupts tracheal branching in the embryo.

      (Mortimer and Moberg, 2009)

      Expression of pntP1.Scer\UAS under the control of Scer\GAL41151 results in an increased number of founder cells compared to the wild-type number in the abdomen.

      (Dutta et al., 2005)

      pntP1.Scer\UAS; Scer\GAL4sca-T3 adult ommatidia do not contain small rhabdomeres, suggesting an absence of R8 photoreceptors. Ommatidia in these eyes also contain a variable number of photoreceptors.

      (Frankfort and Mardon, 2004)

      When expression is driven by Scer\GAL4He.PZ, hemocyte proliferation is increased and lamellocyte and crystal cells numbers are unaffected. Melanotic masses form. Similar, though milder, effects are seen when expression is driven by Scer\GAL4Hml.PG.

      (Zettervall et al., 2004)

      Expression of pntP1.Scer\UAS driven by Scer\GAL4twi.PG, leads to a near complete loss of pericardial and cardioblast cells.

      (Alvarez et al., 2003)

      Expression of pntP1.Scer\UAS under the control of Scer\GAL4ey.PB can result in homeotic transformation of the eye to antenna.

      (Kumar and Moses, 2001)

      Expression of pntP1.Scer\UAS under the control of Scer\GAL4dpp.blk1 results in ectopic furrow formation in the eye disc.

      (Kumar and Moses, 2001)

      Expression of pntP1.Scer\UAS under the control of Scer\GAL4nos.PG results in embryos with defects in the patterning the head skeleton.

      (Tracey et al., 2000)

      In pntP1.Scer\UAS/Scer\GAL471B wings the intervein material between L2 and L3 is lost and wing vein L4 is reduced. If pntP1.Scer\UAS is driven by Scer\GAL4dpp.blk1 increased numbers of macrochaetae are seen on the scutellum. The wings are small and bifurcated. In the wing disc a central cleft is seen.

      (Wessells et al., 1999)

      Scer\GAL4sim.PS-mediated expression causes an increase in number of midline glial cells.

      (Scholz et al., 1997, Scholz et al., 1997)

      Produces pupal lethality when expressed using Scer\GAL4hs.2sev at 25oC. At 17oC, flies expressing pntP1.Scer\UAS using Scer\GAL4hs.2sev eclose, and have rough eyes. The number of rhabdomeres per ommatidium is normal, but ommatidial morphology and orientation is abnormal. Pigment cells are missing between several ommatidia. This phenotype is more pronounced in the posterior part of the eye.

      (Albagli et al., 1996)

      Scer\GAL455B and Scer\GAL4c324a mediated expression produces female progeny that are sterile or semi-sterile and produce eggs that have dorsal defects (no appendages or small 'nubs' of appendage material, 'crumpled' chorion material). Overexpression results in loss of dorsal follicle cells.

      (Morimoto et al., 1996)

      With either Scer\GAL4sca-537.4 or Scer\GAL4rho.PL, there is a reduction or complete lack of commissural connectives. At stages 12-14 ectopic glial cells are evident in the lateral CNS in embryos with Scer\GAL4sca-537.4. These ectopic glial cells are flanked by neurons ectopically expressing the neural antigen 22C10.

      (Klaes et al., 1994)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressed by
      NOT suppressed by
      Statement
      Reference

      Scer\GAL4Act.PU, pntP1.UAS has decreased size | somatic clone | larval stage phenotype, non-suppressible by BacA\p35UAS.cUa, Scer\GAL4Act.PU

      (Ito and Igaki, 2021)

      Scer\GAL4da.Switch.PT, pntP1.UAS has short lived | RU486 conditional phenotype, non-suppressible by chico1/chico[+]

      (Slack et al., 2015)
      Suppressor of
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The increased/ectopic oenocytes in Prd-positive abdominal segments during stage 15 of embryogenesis observed upon the expression of pntP1.Scer\UAS under the control of Scer\GAL4prd.PU is suppressed by the co-expression of EgfrDN.cUa.Scer\UAS, even leading to the complete absence of oenocytes.

      (Wang et al., 2017)

      The increase in the adult midgut intestinal stem cell proliferation characteristic for flies expressing pntP1.Scer\UAS under the control of Scer\GAL4esg-NP5130 (using tub-Gal80[ts] to limit the time of the transgene expression) is not suppressed by co-expression of cicΔC2.Scer\UAS.T:Ivir\HA1.

      (Jin et al., 2015)

      Expression of foxoScer\UAS.cFa enhances the reduction in lifespan seen when pntP1.Scer\UAS is expressed in the adult midgut and fat body under the control of Scer\GAL4Switch1.106 (in the presence of RU486). This phenotype is completely suppressed upon expression of aopACT.Scer\UAS. Co-expression of foxoScer\UAS.cFa and pntP1.Scer\UAS also enhances the reduction in stored triacylglycerol (TAG) levels seen when either transgene is expressed alone. Gut integrity and feeding appear normal.

      Expression of foxoScer\UAS.cFa enhances the reduction in lifespan seen when pntP1.Scer\UAS is expressed in the adult midgut under the control of Scer\GAL4TIGS-2 (in the presence of RU486). This phenotype is partially suppressed upon expression of aopACT.Scer\UAS.

      Flies co-expressing pntP1.Scer\UAS and foxoScer\UAS.cFa under the control of Scer\GAL4Switch1.106 are starvation sensitive after 5 days in the presence of RU486, and this phenotype can be reversed upon expression of aopACT.Scer\UAS.

      (Alic et al., 2014)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4sli.PS/pntP1.UAS rescues pntΔ88

      (Scholz et al., 1997)

      Scer\GAL4sli.PS/pntP1.UAS rescues pntΔ88

      (Scholz et al., 1997)
      Partially rescues

      Scer\GAL4hs.2sev/pntP1.UAS partially rescues pnt1277/pntΔ88

      (Albagli et al., 1996)
      Fails to rescue

      Scer\GAL4twi.PG/pntP1.UAS fails to rescue pntS012309

      (Alvarez et al., 2003)
      Comments

      Partially rescues the eye phenotype of pntΔ88/pnt1277 flies when expressed using Scer\GAL4hs.2sev at 17oC.

      (Albagli et al., 1996)
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      pntP1.Scer\UAS
      pntP1.UAS
      pntP1UAS
      (Pathirana et al., 2001)
      Name Synonyms
      pointedP1
      (Granderath et al., 1999)
      Secondary FlyBase IDs
        References (58)