FlyBase Allele Report: Dmel\EcR[B1-ΔC655.F645A.UAS]

General Information

Symbol

Dmel\EcR^{B1-ΔC655.F645A.UAS}

Species

D. melanogaster

Name

FlyBase ID

FBal0141829

Feature type

allele

Associated gene

Dmel\EcR

Associated Insertion(s)

Carried in Construct

P{UAS-EcR.B1-ΔC655.F645A}

Also Known As

UAS-EcR^DN, UAS-EcR-F645A, UAS-EcR^F645A, EcR-DN, EcR^F645A, UAS-EcR.B1-ΔC655.F645A, UAS-EcR-DN, EcR-F645A, P{UAS-EcR.B1-DeltaC655.F645A}, EcR-B1-F645A

Key Links

Transgenic product class

dominant_negative_variant

(Cherbas et al., 2003)

inframe_deletion

(Cherbas et al., 2003)

missense_variant

(Cherbas et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Cherbas et al., 2003)

inframe_deletion

(Cherbas et al., 2003)

missense_variant

(Cherbas et al., 2003)

Mutagen

in vitro construct

(Cherbas et al., 2003, Cherbas, 2002.12)

Progenitor genotype

Carried in construct

P{UAS-EcR.B1-ΔC655.F645A}

Cytology

Description

UASt regulatory sequences drive expression of a mutated EcR B1 isoform which ends at EcR amino acid residue 655 and has the amino acid replacement F465A.

(Cherbas et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

EcR

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body size | larval stage, with Scer\GAL4^c42

(Gautam et al., 2015)

abnormal developmental rate, with Scer\GAL4^c42

(Gautam et al., 2015)

abnormal developmental rate, with Scer\GAL4^c649

(Gautam and Tapadia, 2010)

abnormal locomotor behavior, with Scer\GAL4^c42

(Gautam et al., 2015)

abnormal neuroanatomy, with Scer\GAL4^alrm.PD

(Tasdemir-Yilmaz and Freeman, 2014)

abnormal neuroanatomy, with Scer\GAL4^FMRFa.PS

(Brown et al., 2006)

abnormal neuroanatomy, with Scer\GAL4^Mhc.PW

(Liu et al., 2010)

abnormal neuroanatomy | larval stage, with Scer\GAL4^RapGAP1-OK6

(Yu et al., 2013, Yu et al., 2013)

abnormal neuroanatomy | pupal stage, with Scer\GAL4^lilli-189Y

(Liou et al., 2018)

abnormal neuroanatomy | pupal stage, with Scer\GAL4^NP3056

(Liou et al., 2018)

abnormal neuroanatomy | pupal stage, with Scer\GAL4^orb-0449-G4

(Liou et al., 2018)

abnormal neuroanatomy | pupal stage, with Scer\GAL4^ppk.PG

(Kuo et al., 2005)

abnormal neuroanatomy | wandering third instar larval stage, with Scer\GAL4^CCAP.PP

(Lee et al., 2019)

abnormal size | pupal stage, with Scer\GAL4^CCAP.PP

(Lee et al., 2019)

decreased body size | larval stage, with Scer\GAL4^c649

(Gautam and Tapadia, 2010)

decreased body size | larval stage, with Scer\GAL4^tsh-c724

(Gautam and Tapadia, 2010)

decreased cell death, with Scer\GAL4^Crz.PC

(Choi et al., 2006)

decreased cell death | heat sensitive, with Scer\GAL4^wg-IS650, Scer\GAL80^ts.αTub84B

(Takemura and Adachi-Yamada, 2011)

decreased cell number | larval stage, with Scer\GAL4^c42

(Gautam et al., 2015)

female sterile, with Scer\GAL4^slbo.2.6

(Cherbas et al., 2003)

increased cell death | wandering third instar larval stage, with Scer\GAL4^CCAP.PP

(Lee et al., 2019)

increased size | adult stage, with Scer\GAL4^byn-Gal4

(Cohen et al., 2021)

lethal, with Scer\GAL4^Act5C.PI

(Cherbas et al., 2003)

lethal, with Scer\GAL4^dpp.blk1

(Gauthier et al., 2012)

lethal, with Scer\GAL4^GMR.PF

(Gauthier et al., 2012, Sedkov et al., 2003)

lethal, with Scer\GAL4^Lsp2.PH

(Gauthier et al., 2012, Cherbas et al., 2003)

lethal, with Scer\GAL4^MsrA.602

(Gauthier et al., 2012)

lethal, with Scer\GAL4^Ser.PGF

(Cherbas et al., 2003)

lethal | embryonic stage, with Scer\GAL4^69B

(Kozlova and Thummel, 2003)

lethal | embryonic stage, with Scer\GAL4^c381

(Kozlova and Thummel, 2003)

lethal | embryonic stage, with Scer\GAL4^{Orct2-cald-GAL4}

(Kozlova and Thummel, 2003)

lethal | heat sensitive, with Scer\GAL4^dpp.blk1

(Cherbas et al., 2003)

lethal | larval stage, with Scer\GAL4^69B

(Kozlova and Thummel, 2003)

lethal | larval stage, with Scer\GAL4^c381

(Kozlova and Thummel, 2003)

lethal | larval stage, with Scer\GAL4^{Orct2-cald-GAL4}

(Kozlova and Thummel, 2003)

lethal - all die before end of first instar larval stage, with Scer\GAL4^C57

(Liu et al., 2010)

lethal - all die before end of first instar larval stage, with Scer\GAL4^tsh-c724

(Gautam and Tapadia, 2010)

lethal - all die before end of larval stage, with Scer\GAL4^c649

(Gautam and Tapadia, 2010)

lethal - all die before end of larval stage, with Scer\GAL4^RapGAP1-OK6

(Liu et al., 2010)

lethal - all die before end of P-stage, with Scer\GAL4^repo

(Liu et al., 2010)

lethal - all die before end of pupal stage, with Scer\GAL4^GMR.PF

(Cherbas et al., 2003)

lethal - all die before end of pupal stage, with Scer\GAL4^Mhc.PW

(Liu et al., 2010)

lethal - all die before end of pupal stage, with Scer\GAL4^Tab2-201Y

(Awasaki and Ito, 2004)

lethal - all die during embryonic stage, with Scer\GAL4^arm.PS

(Colombani et al., 2005, Colombani et al., 2005)

lethal - all die during embryonic stage | heat sensitive, with Scer\GAL4^hs.PB

(Kozlova and Thummel, 2003)

lethal - all die during first instar larval stage, with Scer\GAL4^c42

(Gautam et al., 2015)

long lived | RU486 conditional, with Scer\GAL4^da.Switch.PT

(Tricoire et al., 2009)

short lived, with Scer\GAL4^byn-Gal4

(Cohen et al., 2021)

short lived | female | RU486 conditional, with Scer\GAL4^da.Switch.PT

(Tricoire et al., 2009)

short lived | RU486 conditional, with Scer\GAL4^da.Switch.PT

(Tricoire et al., 2009)

some die during embryonic stage, with Scer\GAL4^arm.PS

(Colombani et al., 2005, Colombani et al., 2005)

some die during embryonic stage | heat sensitive, with Scer\GAL4^hs.PB

(Kozlova and Thummel, 2003)

some die during first instar larval stage, with Scer\GAL4^C57

(Liu et al., 2010)

some die during first instar larval stage, with Scer\GAL4^tsh-c724

(Gautam and Tapadia, 2010)

some die during larval stage, with Scer\GAL4^c649

(Gautam and Tapadia, 2010)

some die during larval stage, with Scer\GAL4^RapGAP1-OK6

(Liu et al., 2010)

some die during pharate adult stage, with Scer\GAL4^repo

(Liu et al., 2010)

some die during pupal stage, with Scer\GAL4^GMR.PF

(Cherbas et al., 2003)

some die during pupal stage, with Scer\GAL4^Mhc.PW

(Liu et al., 2010)

some die during pupal stage, with Scer\GAL4^Tab2-201Y

(Awasaki and Ito, 2004)

viable, with Scer\GAL4^Sgs3.PD

(Cherbas et al., 2003)

viable | somatic clone, with Scer\GAL4^byn-Gal4

(Cohen et al., 2021)

visible, with Scer\GAL4^c42

(Gautam et al., 2015)

visible | pupal stage, with Scer\GAL4^CCAP.PP

(Lee et al., 2019)

Phenotype Manifest In

adult pylorus, with Scer\GAL4^byn-Gal4

(Cohen et al., 2021)

astrocyte-like glial cell, with Scer\GAL4^alrm.PD

(Tasdemir-Yilmaz and Freeman, 2014)

astrocyte-like glial cell | somatic clone, with Scer\GAL4^alrm.PD

(Tasdemir-Yilmaz and Freeman, 2014)

autophagosome, with Scer\GAL4^Lsp2.PH

(Rusten et al., 2004)

border follicle cell, with Scer\GAL4^slbo.2.6

(Jang et al., 2009, Cherbas et al., 2003)

dendritic arborizing neuron | P-stage, with Scer\GAL4^ppk.PG

(Kuo et al., 2005)

dorsal appendage, with Scer\GAL4^slbo.2.6

(Cherbas et al., 2003)

dorsal appendage | maternal effect, with Scer\GAL4^slbo.2.6

(Badenhorst et al., 2005)

egg chorion, with Scer\GAL4^slbo.2.6

(Cherbas et al., 2003)

egg operculum, with Scer\GAL4^slbo.2.6

(Cherbas et al., 2003)

embryo | dorsal closure stage, with Scer\GAL4^hs.PB

(Kozlova and Thummel, 2003)

embryonic/first instar larval cuticle, with Scer\GAL4^c42

(Gautam et al., 2015)

embryonic/first instar larval cuticle, with Scer\GAL4^hs.PB

(Kozlova and Thummel, 2003)

embryonic/larval adipose system, with Scer\GAL4^c42

(Gautam et al., 2015)

embryonic/larval epidermis, with Scer\GAL4^NP0056

(Ninov et al., 2007)

embryonic/larval fat body, with Scer\GAL4^Lsp2.PH

(Cherbas et al., 2003)

embryonic/larval Malpighian tubule, with Scer\GAL4^c42

(Gautam et al., 2015)

embryonic/larval Malpighian tubule | larval stage, with Scer\GAL4^c649

(Gautam and Tapadia, 2010)

embryonic/larval Malpighian tubule | larval stage, with Scer\GAL4^tsh-c724

(Gautam and Tapadia, 2010)

embryonic/larval Malpighian tubule Type I cell, with Scer\GAL4^c42

(Gautam et al., 2015)

embryonic/larval Malpighian tubule Type II cell, with Scer\GAL4^c42

(Gautam et al., 2015)

embryonic/larval Malpighian tubule Type II cell, with Scer\GAL4^c649

(Gautam and Tapadia, 2010)

embryonic/larval Malpighian tubule Type II cell, with Scer\GAL4^tsh-c724

(Gautam and Tapadia, 2010)

embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4^RapGAP1-OK6

(Yu et al., 2013, Yu et al., 2013)

embryonic/larval salivary gland | prepupal stage, with Scer\GAL4^Sgs3.PD

(Ihry and Bashirullah, 2014)

embryonic/larval salivary gland | pupal stage, with Scer\GAL4^Sgs3.PD

(Ihry and Bashirullah, 2014)

embryonic/larval salivary gland | third instar larval stage, with Scer\GAL4^Sgs3.PD

(Ihry and Bashirullah, 2014)

eye, with Scer\GAL4^GMR.PF

(Cherbas et al., 2003, Sedkov et al., 2003, Sedkov et al., 2003)

gamma Kenyon cell, with Scer\GAL4^alrm.PD

(Tasdemir-Yilmaz and Freeman, 2014)

gamma Kenyon cell | somatic clone, with Scer\GAL4^alrm.PD

(Tasdemir-Yilmaz and Freeman, 2014)

germ band, with EcR^M554fs, Scer\GAL4^69B

(Kozlova and Thummel, 2003)

germ band, with EcR^M554fs, Scer\GAL4^c381

(Kozlova and Thummel, 2003)

glial cell & brain & pupa | conditional ts, with Scer\GAL4^Tab2-201Y

(Awasaki and Ito, 2004)

ileum | adult stage, with Scer\GAL4^byn-Gal4

(Cohen et al., 2021)

imaginal disc, with Scer\GAL4^MsrA.657

(Cherbas et al., 2003)

larval CCAP neuron | wandering third instar larval stage, with Scer\GAL4^CCAP.PP

(Lee et al., 2019)

larval transverse nerve, with Scer\GAL4^RapGAP1-OK6

(Yu et al., 2013)

leg | pupal stage, with Scer\GAL4^CCAP.PP

(Lee et al., 2019)

local interneuron of larval antennal lobe | pupal stage, with Scer\GAL4^lilli-189Y

(Liou et al., 2018)

local interneuron of larval antennal lobe | pupal stage, with Scer\GAL4^NP3056

(Liou et al., 2018)

local interneuron of larval antennal lobe | pupal stage, with Scer\GAL4^orb-0449-G4

(Liou et al., 2018)

mesothoracic ventral neurosecretory neuron & axon, with Scer\GAL4^FMRFa.PS

(Brown et al., 2006)

metathoracic ventral thoracic neurosecretory neuron & axon, with Scer\GAL4^FMRFa.PS

(Brown et al., 2006)

mitotic domain 1 | extended germ band stage, with Scer\GAL4^69B

(Kozlova and Thummel, 2003)

mitotic domain 1 | extended germ band stage, with Scer\GAL4^c381

(Kozlova and Thummel, 2003)

mitotic domain 1 | extended germ band stage, with Scer\GAL4^hs.PB

(Kozlova and Thummel, 2003)

mitotic domain 1 | extended germ band stage, with Scer\GAL4^{Orct2-cald-GAL4}

(Kozlova and Thummel, 2003)

mushroom body | P-stage, with Scer\GAL4^Tab2-201Y

(Awasaki and Ito, 2004)

neurohemal organ, with Scer\GAL4^FMRFa.PS

(Brown et al., 2006)

neuron & ventral nerve cord, with Scer\GAL4^Crz.PC

(Choi et al., 2006)

ommatidium, with Scer\GAL4^GMR.PF

(Sedkov et al., 2003)

pigment cell, with Scer\GAL4^GMR.PF

(Cherbas et al., 2003)

polytene chromosome puff, with Scer\GAL4^Sgs3.PD

(Cherbas et al., 2003)

posterior scutellar bristle, with Scer\GAL4^dpp.blk1

(Cherbas et al., 2003)

prothoracic gland | third instar larval stage, with Scer\GAL4^phtm.PO

(Danielsen et al., 2016)

prothoracic ventral neurosecretory neuron & axon, with Scer\GAL4^FMRFa.PS

(Brown et al., 2006)

pupa, with Scer\GAL4^MsrA.657

(Cherbas et al., 2003)

pupal epidermis, with Scer\GAL4^NP0056

(Ninov et al., 2007)

synapse, with Scer\GAL4^alrm.PD

(Tasdemir-Yilmaz and Freeman, 2014)

synapse, with Scer\GAL4^Mhc.PW

(Liu et al., 2010)

wing, with Scer\GAL4^Eh.0.8

(Cherbas et al., 2003)

wing | pupal stage, with Scer\GAL4^CCAP.PP

(Lee et al., 2019)

wing hair | heat sensitive, with Scer\GAL4^wg-IS650, Scer\GAL80^ts.αTub84B

(Takemura and Adachi-Yamada, 2011)

wing margin | heat sensitive, with Scer\GAL4^wg-IS650, Scer\GAL80^ts.αTub84B

(Takemura and Adachi-Yamada, 2011)

Detailed Description

Statement

Reference

The expression of EcR^{B1-ΔC655.F645A.UAS} under the control of Scer\GAL4^byn-Gal4 throughout metamorphosis results in adults that die soon after eclosion, show an enlarged pylorus and lacking ileum histolysis.

(Cohen et al., 2021)

Expressing EcR^{B1-ΔC655.F645A.UAS} under the control of Scer\GAL4^CCAP.PP results in the loss of most CCAP neurons in wandering third instar larvae and severely defective pupal development, characterized by a failure in head eversion and shortened wings and legs.

(Lee et al., 2019)

Expression of EcR^{B1-ΔC655.F645A.UAS} under the control of Scer\GAL4^NP3056, Scer\GAL4^orb-0449-G4 or Scer\GAL4^lilli-189Y in combination with a Gal80[ts] transgene to restrict expression to between late third instar larva and 24 hours after puparium formation, results in the retention of larval innervation patterns of these antennal lobe local interneurons that are lost from controls during metamorphosis.

(Liou et al., 2018)

Expression of EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^phm.PO leads to accumulation of lipid droplets in the prothoracic gland cells of third instar larvae.

(Danielsen et al., 2016)

Expression of EcR^{B1-ΔC655.F645A.Scer\UAS} in salivary glands during embryonic and early larval stages does not affect normal development.

Expression of EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^c42 results in delayed development and larval lethality at the early first instar stage. Larvae are smaller in size, slow moving, and display gross abnormalities including low adipose tissue, transparent cuticle and deformed Malpighian tubules.

Malpighian tubule defects of first instar larvae expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^c42 include reduction in overall size, failure to properly elongate and remaining coiled, smaller mean diameter, reduction of the number of and irregular distribution of stellate cells and principal cells in anterior and posterior Malpighian tubules, and reduction in uric acid deposition as compared to wild type.

(Gautam et al., 2015)

Animals expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^Sgs3.PD show normal mid-third instar (wandering behaviour) and late larval (everting anterior spiracles, barrel shape, midgut programmed cell death) ecdysone-triggered responses. The prepupal ecdysone-triggered responses (head eversion, wing and leg inflation and leg extension) also occur normally. In the salivary gland, mid-third instar glue synthesis, late larval glue secretion and prepupal salivary gland programmed cell death are all defective.

Expression of EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^Sgs3.PD blocks the formation of the large acidic structures which normally occurs in salivary gland cells approximately 1.5 hours after head eversion, and also blocks caspase activation at 2 hours after head eversion. This results in a persistent salivary gland phenotype at 24 hours after puparium formation.

(Ihry and Bashirullah, 2014)

Expression of EcR^{B1-ΔC655.F645A.Scer\UAS} in astrocytes, under the control of Scer\GAL4^alrm.PD, does not affect astrocyte morphology in 3rd instar larvae. However, EcR^{B1-ΔC655.F645A.Scer\UAS} expression completely blocks their morphological transformation into the highly vacuolated morphology apparent in controls at 6 hours after pupal formation.

Astrocyte morphological transformation can be suppressed when EcR^{B1-ΔC655.F645A.Scer\UAS} is expressed in one to two astrocytes, using MARCM, rather than the entire population. EcR^{B1-ΔC655.F645A.Scer\UAS} expression block the induction of phagolysomal activity.

(Tasdemir-Yilmaz and Freeman, 2014)

Expression of EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^RapGAP1-OK6 results in the transverse nerve making ectopic connections at the larval neuromuscular junction.

(Yu et al., 2013)

Expression of EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^RapGAP1-OK6 results in the formation of ectopic connections at the larval neuromuscular junction.

(Yu et al., 2013)

Over 95% of larvae expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^ETH.Switch in the presence of RU486 have multiple mouthparts (indicating a failure to complete ecdysis), and approximately 70% of these animals die as larvae.

(Gauthier et al., 2012)

Severing of the dendrites of ddaC neurons occurs in animals expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^repo does occur, albeit with some delay. However, the glial membranes that wrap the proximal dendrites as part of this dendrite pruning process show very little sign of retraction or disintegration by 16 hours after puparium formation and the wrapped dendrite segments remain intact. This defect persist until the death of the neurons during metamorphosis.

(Han et al., 2011)

Induction of EcR^{B1-ΔC655.F645A.Scer\UAS} during 0-30 hours after pupal formation (under the control of Scer\GAL80^ts.αTub84B and Scer\GAL4^wg-IS650) blocks apoptosis and results in rearrangement of the wing margin cells. In addition, blocking ecdysone signalling results in overlap of the double-row hairs in the adult wing.

(Takemura and Adachi-Yamada, 2011)

Larvae expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of either Scer\GAL4^tsh-c724 or Scer\GAL4^c649 are smaller in size than age-matched controls, with the reduction in size being greatest with the Scer\GAL4^tsh-c724 driver.

Larvae expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^c649 contain both first and second instar larval mouth hooks (showing a defect in moulting) at a time when control larvae have already reached the third larval instar stage.

The mean diameter of the Malpighian tubules of larvae expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of either Scer\GAL4^tsh-c724 or Scer\GAL4^c649 is smaller than that of controls, with the decrease being greatest with the Scer\GAL4^tsh-c724 driver. The number of principal cells in the anterior and posterior Malpighian tubules of the mutant larvae is similar to that in wild type. However, the number of stellate cells in the mutant anterior and posterior Malpighian tubules is reduced compared to wild type, with the reduction being greatest with the Scer\GAL4^tsh-c724 driver. In addition, the distribution of the stellate cells is altered in the mutant tubules, so that they are present in clusters instead of being evenly distributed as occurs in wild type.

(Gautam and Tapadia, 2010)

High expression of EcR^{B1-ΔC655.F645A.Scer\UAS} in muscle under the control of Scer\GAL4^C57 results in first-instar larval lethality. Lower expression under the control of Scer\GAL4^Mhc.PW leads to pupal lethality. Expression under the control of Scer\GAL4^Mhc.PW produces a delay in synapse elimination due to post-synaptic disruption of EcR signaling.

Expression of EcR^{B1-ΔC655.F645A.Scer\UAS} postsynaptically (under the control of Scer\GAL4^Mhc.PW) results in the complete blockage of both pre- and post-synaptic synapse elimination in pupae 9 hours after puparium formation.

(Liu et al., 2010)

Flies expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^Lsp2.PH have a normal total lipid content.

(Wang et al., 2010)

Expression of EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^slbo.2.6 results in incomplete border cell migration in 60% of stage 10 egg chambers at 29[o]C.

(Jang et al., 2009)

Expression of EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^da.Switch.PT in the presence of 1μg ml[-1] RU486 in male flies results in a 24% increase in the median lifespan compared to controls. Higher levels of EcR^{B1-ΔC655.F645A.Scer\UAS} expression (using increasing concentrations of RU486) abolish this positive effect.

Expression of EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^da.Switch.PT in the presence of RU486 in female flies decreases lifespan in a RU486 dose-dependent manner.

(Tricoire et al., 2009)

Expression of EcR^{B1-ΔC655.F645A.Scer\UAS} in larval epidermal cells, under the control of Scer\GAL4^NP0056, blocks the ability of these cells to constrict, extrude and undergo apoptosis. As a result, abdominal morphogenesis does not succeed and larval epidermal cells remain in the abdomen of pharate adult flies, forming part of the epidermis.

(Ninov et al., 2007)

Thoracic ventral neurosecretory cells that express EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^Fmrf.PS are of normal size and larval morphology at the start of metamorphosis. However, these mutants show defective neuronal remodelling during metamorphosis. The axons show much lower levels of pruning compared to wild-type cells and show no filopodia during the pruning phase. EcR^{B1-ΔC655.F645A.Scer\UAS}-expressing neurons start extending filopodia after 24 hours APF from the neurohemal organ and in the growth zone below. As this happens, the remnant neurohemal organ partially fragments, resulting in an adult-like arbor with clumped varicosities and a dense centre. Effects on adult axon outgrowth are relatively mild, with axonal arbors slighlty reduced compared to controls.

(Brown et al., 2006)

Expression of EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^Crz blocks programmed cell death in Crz-expressing ventral nerve cord neurons up to 12 hours after pupal formation.

(Choi et al., 2006)

23% of embryos derived from females carrying EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^slbo.2.6 have abnormal dorsal appendages.

(Badenhorst et al., 2005)

When EcR^{B1-ΔC655.F645A.Scer\UAS} is driven by Scer\GAL4^arm.PS 100% lethality is seen. at 18^oC this occurs during first instar larval stage, at 25^oC this can be embryonic.

(Colombani et al., 2005, Colombani et al., 2005)

Inhibition of ecdysone signalling by expression of EcR^{B1-ΔC655.F645A.Scer\UAS} under the regulation of Scer\GAL4^ppk.PG results in C4da neurons losing their ability to initiate larval dendrite pruning at 20 hours after pupal formation, with approximately 90% of large dendritic branches being retained after head eversion.

(Kuo et al., 2005)

Inhibition of EcR, through expression of EcR^{B1-ΔC655.F645A.Scer\UAS} at the onset of metamorphosis through the regulatory control of Scer\GAL4^how-24B and Scer\GAL80^ts.αTub84B results in myofibrils that are transversal, indicating that EcR^{B1-ΔC655.F645A.Scer\UAS} prevents terminal chamber differentiation, with nerve terminals not making synaptic contact with the myocytes.

(Monier et al., 2005)

Expression of EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^Tab2-201Y results in lethality at late pupal stages. Mushroom body axons are not pruned during pupal development in these animals (in contrast to wild type). Infiltration of glial processes into the mushroom body lobes is severely suppressed during early pupal development compared to wild-type pupae.

(Awasaki and Ito, 2004)

When expression is governed by Scer\GAL4^Lsp2.PH in the larval fat body early autophagosomal structures are able to form but do not acidify, perhaps due to a failure to fuse with lysosomes.

(Rusten et al., 2004)

Expression of EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^Act5C.PI results in lethality either during embryonic development or soon after hatching. Expression of EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^GMR.PF usually results in animals ceasing development after pupariation. Escapers have defective eyes, with poorly pigmented ommatidia and large necrotic, melanised patches. At 25^oC, most animals expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^dpp.blk1 fail to pupate. At 20^oC, some animals arrest before pupation, while most pupate but die during adult development. Escapers are morphologically normal except for the orientation and sometimes length of the posterior scutellar bristles. Animals expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^Ser.PGF fail to pupate. Pupariation is defective in animals expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^Eip71CD.657; the animals fail to shorten and tanning occurs only at the extremities (where the Scer\GAL4^Eip71CD.657 driver is not expressed). The imaginal discs do not evert. The salivary glands histolyse on schedule at pupation in animals expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^Sgs3.PD. The polytene chromosomes are normal in size, but their puffing pattern in white prepupae remains blocked at puff stage 1' (PS1'), a stage normally seen only early in the third larval instar. The fat body cells fail to dissociate at pupation in animals expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^Lsp2.PH. Most of the animals die late in adult development. Viability is lower in females than in males. Most animals expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^Eh.0.8 moult into adult but fail to expand their wings. Border cell migration is delayed or fails completely in females expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^slbo.2.6. The chorion structures are fragile and eggs derived from these females collapse soon after oviposition. Dorsal appendages are frequently broad or branched and operculum structures are poorly developed.

(Cherbas et al., 2003)

Expression of EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^hs.PB using heat shock for 3 to 5 hours after egg laying results in embryonic lethality, with highly penetrant defects in germ band retraction, head involution and cuticle deposition. Expression of EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^LP1, Scer\GAL4^69B or Scer\GAL4^c381 results in embryonic and first larval instar lethality with a low penetrance of germ band retraction defects. Expression of EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^69B or Scer\GAL4^c381 in an EcR^M554fs/+ background results in 23% or 15% respectively of the embryos having germ band retraction defects.

(Kozlova and Thummel, 2003)

When EcR^{B1-ΔC655.F645A.Scer\UAS} is driven by Scer\GAL4^GMR.PF massive necrosis is seen in the eye, resulting in a collapse of the domed shape of the eye, a large decrease in the level of pigmentation. poorly defined facets and a very characteristic scar.

(Sedkov et al., 2003)

When EcR^{B1-ΔC655.F645A.Scer\UAS} is driven by Scer\GAL4^GMR.PF defects are seen in eye differentiation and a marked lethality is seen before adult eclosion.

(Sedkov et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

EcR^{B1-ΔC655.F645A.UAS}, Scer\GAL4^MsrA.602 has lethal phenotype, enhanceable by crc¹/crc[+]

(Gauthier et al., 2012)

EcR^{B1-ΔC655.F645A.UAS}, Scer\GAL4^GMR.PF has lethal phenotype, enhanceable by trr³

(Sedkov et al., 2003)

EcR^{B1-ΔC655.F645A.UAS}, Scer\GAL4^GMR.PF has lethal phenotype, enhanceable by trr¹

(Sedkov et al., 2003)

NOT Enhanced by

Statement

Reference

EcR^{B1-ΔC655.F645A.UAS}, Scer\GAL4^GMR.PF has lethal phenotype, non-enhanceable by crc¹/crc[+]

(Gauthier et al., 2012)

Suppressed by

Statement

Reference

EcR^{B1-ΔC655.F645A.UAS}, Scer\GAL4^CCAP.PP has visible | pupal stage phenotype, suppressible by BacA\p35^UAS.cUa, Scer\GAL4^CCAP.PP

(Lee et al., 2019)

EcR^{B1-ΔC655.F645A.UAS}, Scer\GAL4^CCAP.PP has abnormal size | pupal stage phenotype, suppressible by BacA\p35^UAS.cUa, Scer\GAL4^CCAP.PP

(Lee et al., 2019)

EcR^{B1-ΔC655.F645A.UAS}, Scer\GAL4^CCAP.PP has increased cell death | wandering third instar larval stage phenotype, suppressible by BacA\p35^UAS.cUa, Scer\GAL4^CCAP.PP

(Lee et al., 2019)

EcR^{B1-ΔC655.F645A.UAS}, Scer\GAL4^CCAP.PP has abnormal neuroanatomy | wandering third instar larval stage phenotype, suppressible by BacA\p35^UAS.cUa, Scer\GAL4^CCAP.PP

(Lee et al., 2019)

EcR^{B1-ΔC655.F645A.UAS}, Scer\GAL4^dpp.blk1 has lethal phenotype, suppressible | partially by crc¹/crc[+]

(Gauthier et al., 2012)

EcR^{B1-ΔC655.F645A.UAS}, Scer\GAL4^Lsp2.PH has lethal phenotype, suppressible | partially by crc¹/crc[+]

(Gauthier et al., 2012)

EcR^{B1-ΔC655.F645A.UAS}, Scer\GAL4^GMR.PF has lethal phenotype, suppressible | partially by Smr^BG01648

(Sedkov et al., 2003)

EcR^{B1-ΔC655.F645A.UAS}, Scer\GAL4^GMR.PF has lethal phenotype, suppressible | partially by trr^+t12

(Sedkov et al., 2003)

NOT suppressed by

Statement

Reference

EcR^{B1-ΔC655.F645A.UAS}, Scer\GAL4^GMR.PF has lethal phenotype, non-suppressible by crc¹/crc[+]

(Gauthier et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

EcR^{B1-ΔC655.F645A.UAS}, Scer\GAL4^slbo.2.6 has egg phenotype, enhanceable by crc¹/crc[+]

(Gauthier et al., 2012)

EcR^{B1-ΔC655.F645A.UAS}, Scer\GAL4^slbo.2.6 has dorsal appendage | maternal effect phenotype, enhanceable by E(bx)^Nurf301-2/E(bx)[+]

(Badenhorst et al., 2005)

EcR^{B1-ΔC655.F645A.UAS}, Scer\GAL4^slbo.2.6 has dorsal appendage | maternal effect phenotype, enhanceable by Iswi[+]/Iswi¹

(Badenhorst et al., 2005)

EcR^{B1-ΔC655.F645A.UAS}, Scer\GAL4^slbo.2.6 has dorsal appendage | maternal effect phenotype, enhanceable by Iswi[+]/Iswi²

(Badenhorst et al., 2005)

EcR^{B1-ΔC655.F645A.UAS}, Scer\GAL4^slbo.2.6 has dorsal appendage | maternal effect phenotype, enhanceable by Nurf-38¹/Nurf-38[+]

(Badenhorst et al., 2005)

EcR^{B1-ΔC655.F645A.UAS}, Scer\GAL4^slbo.2.6 has dorsal appendage | maternal effect phenotype, enhanceable by E(bx)^ry122/E(bx)[+]

(Badenhorst et al., 2005)

Suppressed by

Statement

Reference

EcR^{B1-ΔC655.F645A.UAS}, Scer\GAL4^CCAP.PP has leg | pupal stage phenotype, suppressible by BacA\p35^UAS.cUa, Scer\GAL4^CCAP.PP

(Lee et al., 2019)

EcR^{B1-ΔC655.F645A.UAS}, Scer\GAL4^CCAP.PP has wing | pupal stage phenotype, suppressible by BacA\p35^UAS.cUa, Scer\GAL4^CCAP.PP

(Lee et al., 2019)

EcR^{B1-ΔC655.F645A.UAS}, Scer\GAL4^CCAP.PP has larval CCAP neuron | wandering third instar larval stage phenotype, suppressible by BacA\p35^UAS.cUa, Scer\GAL4^CCAP.PP

(Lee et al., 2019)

EcR^{B1-ΔC655.F645A.UAS}, Scer\GAL4^slbo.2.6 has border follicle cell phenotype, suppressible | partially by ab[+]/ab^1D

(Jang et al., 2009)

EcR^{B1-ΔC655.F645A.UAS}, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Smr^BG01648

(Sedkov et al., 2003)

EcR^{B1-ΔC655.F645A.UAS}, Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible by Smr^BG01648

(Sedkov et al., 2003)

EcR^{B1-ΔC655.F645A.UAS}, Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible by trr^+t12

(Sedkov et al., 2003)

EcR^{B1-ΔC655.F645A.UAS}, Scer\GAL4^GMR.PF has eye phenotype, suppressible by trr^+t12

(Sedkov et al., 2003)

EcR^{B1-ΔC655.F645A.UAS}, Scer\GAL4^GMR.PF has eye phenotype, suppressible | partially by Smr^BG01648

(Sedkov et al., 2003)

EcR^{B1-ΔC655.F645A.UAS}, Scer\GAL4^GMR.PF has eye phenotype, suppressible | partially by trr^+t12

(Sedkov et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

The presence of ab^1D/+ reduces the frequency of incomplete border migration in animals expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^slbo.2.6 to 34% of stage 10 egg chambers.

(Jang et al., 2009)

The deleterious effect on female lifespan of expression of EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^da.Switch.PT in the presence of RU486 is suppressed by ovo^D1.

(Tricoire et al., 2009)

The frequency of abnormal dorsal appendages in embryos derived from females carrying EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^slbo.2.6 (23%) is increased if the females are also heterozygous for one of the following alleles: E(bx)^ry122 (84%), E(bx)^Nurf301-2 (70%), Iswi¹ (76%), Iswi² (45%) or Nurf-38¹ (69%).

(Badenhorst et al., 2005)

The addition of trr^+t12 or Smr^BG01648 to EcR^{B1-ΔC655.F645A.Scer\UAS}, Scer\GAL4^GMR.PF animals causes a dramatic decrease in the level of necrosis and scarring and some improvement of the morphology of the facets.

(Sedkov et al., 2003)

The lethality seen in EcR^{B1-ΔC655.F645A.Scer\UAS}, Scer\GAL4^GMR.PF animals is enhanced by the addition of trr¹/+ or trr³/+. The lethality and the EcR^{B1-ΔC655.F645A.Scer\UAS}, Scer\GAL4^GMR.PF eye phenotype is partially suppressed by trr^+t12. The lethality and the EcR^{B1-ΔC655.F645A.Scer\UAS}, Scer\GAL4^GMR.PF eye phenotype is partially suppressed by Smr^BG01648.

(Sedkov et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

EcR^{B1-ΔC655.F645A.UAS} is rescued by Scer\GAL4^ETH.Switch/EcR^B2-E9K.UAS

(Gauthier et al., 2012)

EcR^{B1-ΔC655.F645A.UAS} is rescued by Scer\GAL4^ETH.Switch/EcR^B2.UAS

(Gauthier et al., 2012)

EcR^{B1-ΔC655.F645A.UAS} is rescued by Scer\GAL4^tsh-c724/EcR^B2.UAS

(Gautam and Tapadia, 2010)

EcR^{B1-ΔC655.F645A.UAS} is rescued by Scer\GAL4^c649/EcR^B2.UAS

(Gautam and Tapadia, 2010)

EcR^{B1-ΔC655.F645A.UAS} is rescued by EcR^B1.UAS/Scer\GAL4^ppk.PG

(Kuo et al., 2005)

EcR^{B1-ΔC655.F645A.UAS} is rescued by EcR^B1.UAS/Scer\GAL4^Eh.0.8

(Cherbas et al., 2003)

EcR^{B1-ΔC655.F645A.UAS} is rescued by EcR^B1.UAS/Scer\GAL4^Lsp2.PH

(Cherbas et al., 2003)

EcR^{B1-ΔC655.F645A.UAS} is rescued by EcR^B1.UAS/Scer\GAL4^GMR.PF

(Cherbas et al., 2003)

EcR^{B1-ΔC655.F645A.UAS} is rescued by Scer\GAL4^Eh.0.8/EcR^B2.UAS

(Cherbas et al., 2003)

EcR^{B1-ΔC655.F645A.UAS} is rescued by Scer\GAL4^Lsp2.PH/EcR^B2.UAS

(Cherbas et al., 2003)

EcR^{B1-ΔC655.F645A.UAS} is rescued by Scer\GAL4^GMR.PF/EcR^B2.UAS

(Cherbas et al., 2003)

EcR^{B1-ΔC655.F645A.UAS} is rescued by Scer\GAL4^Eh.0.8/EcR^A.UAS

(Cherbas et al., 2003)

EcR^{B1-ΔC655.F645A.UAS} is rescued by Scer\GAL4^Lsp2.PH/EcR^A.UAS

(Cherbas et al., 2003)

EcR^{B1-ΔC655.F645A.UAS} is rescued by Scer\GAL4^Ser.PGF/EcR^A.UAS

(Cherbas et al., 2003)

EcR^{B1-ΔC655.F645A.UAS} is rescued by Scer\GAL4^GMR.PF/EcR^A.UAS

(Cherbas et al., 2003)

EcR^{B1-ΔC655.F645A.UAS} is rescued by Scer\GAL4^Lsp2.PH/EcR^C.UAS

(Cherbas et al., 2003)

EcR^{B1-ΔC655.F645A.UAS} is rescued by EcR^C.UAS/Scer\GAL4^GMR.PF

(Cherbas et al., 2003)

Partially rescued by

EcR^{B1-ΔC655.F645A.UAS} is partially rescued by Scer\GAL4^c42/EcR^A.UAS

(Gautam et al., 2015)

EcR^{B1-ΔC655.F645A.UAS} is partially rescued by EcR^B1.UAS/Scer\GAL4^c42

(Gautam et al., 2015)

EcR^{B1-ΔC655.F645A.UAS} is partially rescued by Scer\GAL4^c42/EcR^B2.UAS

(Gautam et al., 2015)

EcR^{B1-ΔC655.F645A.UAS} is partially rescued by EcR^B1.UAS/Scer\GAL4^ETH.Switch

(Gauthier et al., 2012)

EcR^{B1-ΔC655.F645A.UAS} is partially rescued by Scer\GAL4^ETH.Switch/EcR^A.UAS

(Gauthier et al., 2012)

EcR^{B1-ΔC655.F645A.UAS} is partially rescued by EcR^B1.UAS/Scer\GAL4^Sgs3.PD

(Cherbas et al., 2003)

EcR^{B1-ΔC655.F645A.UAS} is partially rescued by EcR^B1.UAS/Scer\GAL4^dpp.blk1

(Cherbas et al., 2003)

EcR^{B1-ΔC655.F645A.UAS} is partially rescued by Scer\GAL4^MsrA.602/EcR^B2.UAS

(Cherbas et al., 2003)

EcR^{B1-ΔC655.F645A.UAS} is partially rescued by Scer\GAL4^Sgs3.PD/EcR^B2.UAS

(Cherbas et al., 2003)

EcR^{B1-ΔC655.F645A.UAS} is partially rescued by Scer\GAL4^dpp.blk1/EcR^B2.UAS

(Cherbas et al., 2003)

EcR^{B1-ΔC655.F645A.UAS} is partially rescued by EcR^B2.UAS/Scer\GAL4^slbo.2.6

(Cherbas et al., 2003)

EcR^{B1-ΔC655.F645A.UAS} is partially rescued by Scer\GAL4^Sgs3.PD/EcR^A.UAS

(Cherbas et al., 2003)

EcR^{B1-ΔC655.F645A.UAS} is partially rescued by Scer\GAL4^dpp.blk1/EcR^A.UAS

(Cherbas et al., 2003)

EcR^{B1-ΔC655.F645A.UAS} is partially rescued by Scer\GAL4^dpp.blk1/EcR^C.UAS

(Cherbas et al., 2003)

Not rescued by

EcR^{B1-ΔC655.F645A.UAS} is not rescued by EcR^B1.UAS/Scer\GAL4^MsrA.602

(Cherbas et al., 2003)

EcR^{B1-ΔC655.F645A.UAS} is not rescued by EcR^B1.UAS/Scer\GAL4^slbo.2.6

(Cherbas et al., 2003)

EcR^{B1-ΔC655.F645A.UAS} is not rescued by Scer\GAL4^MsrA.602/EcR^A.UAS

(Cherbas et al., 2003)

EcR^{B1-ΔC655.F645A.UAS} is not rescued by Scer\GAL4^slbo.2.6/EcR^A.UAS

(Cherbas et al., 2003)

Comments

Co-expression of EcR^A.Scer\UAS postpones larval lethality from the 1st instar to the 2nd/3rd instar transition, but results in little-to-no rescue of the Malpighian tubule phenotypes of EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^c42. Cuticle preparations of these larvae show molting defects.

Co-expression of EcR^B1.Scer\UAS results in partial rescue of larval lethality, and significant rescue of the Malpighian tubule phenotypes of EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^c42.

Co-expression of EcR^B2.Scer\UAS results in nearly complete rescue of larval lethality and Malpighian tubule phenotypes of EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^c42.

(Gautam et al., 2015)

Expression of either EcR^B2.Scer\UAS or EcR^{B2-E9K.Scer\UAS} under the control of Scer\GAL4^ETH.Switch in the presence of RU486 fully rescues the lethality and failure of ecdysis phenotypes seen in larvae expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^ETH.Switch in the presence of RU486.

Expression of either EcR^A.Scer\UAS or EcR^B1.Scer\UAS under the control of Scer\GAL4^ETH.Switch in the presence of RU486 partially rescues the lethality and failure of ecdysis phenotypes seen in larvae expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^ETH.Switch in the presence of RU486.

(Gauthier et al., 2012)

Co-expression of EcR^B2.Scer\UAS rescues the Malpighian tubule defects (reduction in diameter of the tubules and reduction in number and change in distribution of stellate cells) seen in larvae expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of either Scer\GAL4^tsh-c724 or Scer\GAL4^c649.

(Gautam and Tapadia, 2010)

Co-expression of EcR^B1.Scer\UAS with EcR^{B1-ΔC655.F645A.Scer\UAS} in C4da neurons, under the regulation of Scer\GAL4^ppk.1.9 results in the complete rescue of EcR^{B1-ΔC655.F645A.Scer\UAS}-related dendritic pruning defects in C4da neurons.

Co-expression of EcR^B1.Scer\UAS with EcR^{B1-ΔC655.F645A.Scer\UAS} in C4da neurons, under the regulation of Scer\GAL4^ppk.PG results in the complete rescue of EcR^{B1-ΔC655.F645A.Scer\UAS}-related dendritic pruning defects in C4da neurons.

(Kuo et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6869

w¹¹¹⁸; P{UAS-EcR.B1-ΔC655.F645A}TP1

Notes on Origin

Discoverer

Comments

Encodes a dominant negative form of EcR.

(Cherbas et al., 2003)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

EcR^{B1-ΔC655.F645A.Scer\UAS}

EcR^{B1-ΔC655.F645A.UAS}

EcR^F645A

(Lee et al., 2019, Gautam and Tapadia, 2010, Okamoto et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (52)

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