FlyBase Allele Report: Dmel\Rab11[UAS.EGFP]

General Information

Symbol

Dmel\Rab11^UAS.EGFP

Species

D. melanogaster

Name

FlyBase ID

FBal0156007

Feature type

allele

Associated gene

Dmel\Rab11

Associated Insertion(s)

Carried in Construct

P{UAS-Rab11-GFP}

Also Known As

UAS-Rab11-GFP, UAS-GFP-Rab11, Rab11-GFP, UASRab11-GFP, GFP-Rab11

Key Links

Transgenic product class

wild_type

(Chang, 2004.6)

Mutagen

Nature of the Allele

Transgenic product class

wild_type

(Chang, 2004.6)

Mutagen

in vitro construct - regulatory fusion

(Chang, 2004.6)

in vitro construct - coding region fusion

(Chang, 2004.6)

Progenitor genotype

Carried in construct

P{UAS-Rab11-GFP}

Cytology

Description

The Rab11 coding sequence is fused to EGFP; expression is governed by UAS regulatory sequences.

(Chang, 2004.6)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Rab11

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cholera

modeled by Vcho\ctxA^UAS.cGa

(Guichard et al., 2013)

ameliorates Huntington's disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Steinert et al., 2012)

ameliorates Parkinson's disease

modeled by Hsap\SNCA^UAS.cFa

(Breda et al., 2015)

modeled by Hsap\SNCA^UAS.cKa

(Breda et al., 2015)

ameliorates neurodegenerative disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Richards et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult midgut epithelium, with Scer\GAL4^NP0001

(Guichard et al., 2013)

Detailed Description

Statement

Reference

Expression of Rab11^{dsRNA.UAS.WIZ} under the control of Scer\GAL4^Ugt36A1.PK) leads to mild slit diagram localization defects.

(Kampf et al., 2019)

Expression of Rab11^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Bx-MS1096 has no effect on wing development.

Sucrose dye fed to flies expressing Rab11^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^{Myo31DF-NP0001} leaks into the body cavity, indicating that the epithelial barrier is breached.

Expression of Rab11^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^{Myo31DF-NP0001} increases the survival of flies infected with Vibrio cholerae (both wild-type and &ctxa V. cholerae strains).

(Guichard et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

FBal0156007:, Hsap\SNCA^UAS.cFa, Scer\GAL4^ple.PF has abnormal locomotor behavior | adult stage phenotype, suppressible | partially by Rab11^UAS.EGFP/Rab11^UAS.EGFP

(Breda et al., 2015)

Enhancer of

Statement

Reference

Rab11^UAS.EGFP, Scer\GAL4^Bx-MS1096 is an enhancer of visible phenotype of Scer\GAL4^Bx-MS1096, Vcho\ctxA^UAS.cGa

(Guichard et al., 2013)

Suppressor of

Statement

Reference

Rab11^UAS.EGFP, Scer\GAL4^ninaE.PT is a suppressor of decreased cell number | adult stage | progressive phenotype of Hsap\BIN1^UAS.1, Scer\GAL4^ninaE.PT

(Lambert et al., 2022)

Rab11^UAS.EGFP, Scer\GAL4^C164 is a suppressor of abnormal locomotor behavior | third instar larval stage phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^C164

(Breda et al., 2015)

Rab11^UAS.EGFP, Scer\GAL4^ple.PF is a suppressor | partially of abnormal locomotor behavior | third instar larval stage phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^ple.PF

(Breda et al., 2015)

Rab11^UAS.EGFP/Rab11^UAS.EGFP is a suppressor | partially of abnormal locomotor behavior | adult stage phenotype of FBal0156007:, Hsap\SNCA^UAS.cFa, Scer\GAL4^ple.PF

(Breda et al., 2015)

Rab11^UAS.EGFP, Scer\GAL4^elav-C155 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Hsap\SNCA^UAS.cKa, Scer\GAL4^elav-C155

(Breda et al., 2015)

Rab11^UAS.EGFP, Scer\GAL4^elav-C155 is a suppressor of abnormal neurophysiology | third instar larval stage phenotype of Hsap\SNCA^UAS.cKa, Scer\GAL4^elav-C155

(Breda et al., 2015)

Rab11^UAS.EGFP, Scer\GAL4^C164 is a suppressor of abnormal locomotor behavior | third instar larval stage phenotype of Hsap\SNCA^UAS.cKa, Scer\GAL4^C164

(Breda et al., 2015)

Rab11^UAS.EGFP, Scer\GAL4^ple.PF is a suppressor of abnormal locomotor behavior | third instar larval stage phenotype of Hsap\SNCA^UAS.cKa, Scer\GAL4^ple.PF

(Breda et al., 2015)

Scer\GAL4^elav-C155/Rab11^UAS.EGFP is a suppressor of abnormal neuroanatomy | adult stage | progressive phenotype of Hsap\SNCA^UAS.cKa

(Breda et al., 2015)

Rab11^UAS.EGFP, Scer\GAL4^elav-C155 is a suppressor of abnormal locomotor behavior | adult stage phenotype of Hsap\SNCA^UAS.cKa, Scer\GAL4^elav-C155

(Breda et al., 2015)

Rab11^UAS.EGFP, Scer\GAL4^elav-C155 is a suppressor of short lived | adult stage phenotype of Hsap\SNCA^UAS.cKa, Scer\GAL4^elav-C155

(Breda et al., 2015)

Scer\GAL4^elav-C155/Rab11^UAS.EGFP is a suppressor of abnormal neuroanatomy | adult stage | progressive phenotype of Hsap\SNCA^UAS.cFa

(Breda et al., 2015)

Scer\GAL4^ple.PF/Rab11^UAS.EGFP is a suppressor of abnormal neuroanatomy | adult stage | progressive phenotype of Hsap\SNCA^UAS.cFa

(Breda et al., 2015)

Rab11^UAS.EGFP, Scer\GAL4^elav-C155 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^elav-C155

(Breda et al., 2015)

Rab11^UAS.EGFP, Scer\GAL4^elav-C155 is a suppressor of abnormal neurophysiology | third instar larval stage phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^elav-C155

(Breda et al., 2015)

Rab11^UAS.EGFP, Scer\GAL4^elav-C155 is a suppressor of abnormal locomotor behavior | adult stage phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^elav-C155

(Breda et al., 2015)

Rab11^UAS.EGFP, Scer\GAL4^elav-C155 is a suppressor of short lived | adult stage phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^elav-C155

(Breda et al., 2015)

Rab11^UAS.EGFP, Scer\GAL4^Bx-MS1096 is a suppressor | partially of visible phenotype of Scer\GAL4^Bx-MS1096, Vcho\ctxA^UAS.cGa

(Guichard et al., 2013)

Scer\GAL80^ts.αTub84B, Rab11^UAS.EGFP, Scer\GAL4^NP0001 is a suppressor of visible phenotype of Scer\GAL4^NP0001, Scer\GAL80^ts.αTub84B, Vcho\ctxA^UAS.cGa

(Guichard et al., 2013)

Rab11^UAS.EGFP, Scer\GAL4^elav-C155 is a suppressor of abnormal neuroanatomy phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Steinert et al., 2012)

Rab11^UAS.EGFP, Scer\GAL4^elav-C155 is a suppressor of abnormal neurophysiology phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Steinert et al., 2012)

Scer\GAL4^elav-C155/Rab11^UAS.EGFP is a suppressor of abnormal locomotor behavior | third instar larval stage phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^C164

(Steinert et al., 2012)

Rab11^UAS.EGFP, Scer\GAL4^elav-C155 is a suppressor of some die during pupal stage phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Richards et al., 2011)

Rab11^UAS.EGFP, Scer\GAL4^elav-C155 is a suppressor of short lived phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Richards et al., 2011)

NOT Suppressor of

Statement

Reference

Rab11^UAS.EGFP, Scer\GAL4^CCAP.PP is a non-suppressor of visible | adult stage phenotype of Rab2^KK107630, Scer\GAL4^CCAP.PP

(Lund et al., 2021)

Other

Statement

Reference

Rab11^UAS.EGFP, Scer\GAL4^Toll-6-D42, ccb^RNAi.UAS.cMa has abnormal neuroanatomy | larval stage phenotype

(Martin-Peña and Ferrus, 2020)

Rab11^UAS.EGFP, Scer\GAL4^Toll-6-D42, ccb^RNAi.UAS.cMa has decreased size | larval stage phenotype

(Martin-Peña and Ferrus, 2020)

Phenotype Manifest In

Enhancer of

Statement

Reference

Rab11^UAS.EGFP, Scer\GAL4^Bx-MS1096 is an enhancer of wing phenotype of Scer\GAL4^Bx-MS1096, Vcho\ctxA^UAS.cGa

(Guichard et al., 2013)

Rab11^UAS.EGFP, Scer\GAL4^sca.PU is an enhancer of scutellar bristle phenotype of IKKε^DN.UAS, Scer\GAL4^sca.PU

(Otani et al., 2011)

Suppressor of

Statement

Reference

Rab11^UAS.EGFP, Scer\GAL4^ninaE.PT is a suppressor of outer photoreceptor cell | adult stage | decreased number phenotype of Hsap\BIN1^UAS.1, Scer\GAL4^ninaE.PT

(Lambert et al., 2022)

Rab11^UAS.EGFP, Scer\GAL4^elav-C155 is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Hsap\SNCA^UAS.cKa, Scer\GAL4^elav-C155

(Breda et al., 2015)

Scer\GAL4^elav-C155/Rab11^UAS.EGFP is a suppressor of NMJ bouton | third instar larval stage phenotype of Hsap\SNCA^UAS.cKa

(Breda et al., 2015)

Rab11^UAS.EGFP, Scer\GAL4^elav-C155 is a suppressor of synaptic vesicle | third instar larval stage phenotype of Hsap\SNCA^UAS.cKa, Scer\GAL4^elav-C155

(Breda et al., 2015)

Rab11^UAS.EGFP, Scer\GAL4^elav-C155 is a suppressor of dopaminergic PPL1 neuron | adult stage phenotype of Hsap\SNCA^UAS.cKa, Scer\GAL4^elav-C155

(Breda et al., 2015)

Rab11^UAS.EGFP, Scer\GAL4^elav-C155 is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^elav-C155

(Breda et al., 2015)

Rab11^UAS.EGFP, Scer\GAL4^elav-C155 is a suppressor of NMJ bouton | third instar larval stage phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^elav-C155

(Breda et al., 2015)

Rab11^UAS.EGFP, Scer\GAL4^elav-C155 is a suppressor of synaptic vesicle | third instar larval stage phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^elav-C155

(Breda et al., 2015)

Rab11^UAS.EGFP, Scer\GAL4^elav-C155 is a suppressor of dopaminergic PPL1 neuron | adult stage phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^elav-C155

(Breda et al., 2015)

Scer\GAL4^elav-C155/Rab11^UAS.EGFP is a suppressor of dopaminergic PPL1 neuron | adult stage phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^ple.PF

(Breda et al., 2015)

Rab11^UAS.EGFP, Scer\GAL4^GMR.PU is a suppressor | partially of rhabdomere of eye photoreceptor cell | adult stage phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^GMR.PU

(Breda et al., 2015)

Rab11^UAS.EGFP, Scer\GAL4^Bx-MS1096 is a suppressor | partially of wing phenotype of Scer\GAL4^Bx-MS1096, Vcho\ctxA^UAS.cGa

(Guichard et al., 2013)

Rab11^UAS.EGFP, Scer\GAL4^NP0001 is a suppressor | partially of adult midgut epithelium phenotype of Scer\GAL4^NP0001, Vcho\ctxA^UAS.cGa

(Guichard et al., 2013)

Rab11^UAS.EGFP, Scer\GAL4^elav-C155 is a suppressor of NMJ bouton phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Steinert et al., 2012)

Rab11^UAS.EGFP, Scer\GAL4^elav-C155 is a suppressor of synaptic vesicle phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Steinert et al., 2012)

Rab11^UAS.EGFP, Scer\GAL4^elav-C155 is a suppressor of rhabdomere phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Richards et al., 2011)

Rab11^UAS.EGFP, Scer\GAL4^Bx-MS1096 is a suppressor of wing phenotype of Bant\EF^UAS.cGa, Scer\GAL4^Bx-MS1096

(Guichard et al., 2010)

NOT Suppressor of

Statement

Reference

Rab11^UAS.EGFP, Scer\GAL4^CCAP.PP is a non-suppressor of wing phenotype of Rab2^KK107630, Scer\GAL4^CCAP.PP

(Lund et al., 2021)

Other

Statement

Reference

Rab11^UAS.EGFP, Scer\GAL4^Toll-6-D42, ccb^RNAi.UAS.cMa has embryonic/larval neuromuscular junction phenotype

(Martin-Peña and Ferrus, 2020)

Rab11^UAS.EGFP, Scer\GAL4^Toll-6-D42, ccb^RNAi.UAS.cMa has A1-7 ventral longitudinal muscle cell | larval stage phenotype

(Martin-Peña and Ferrus, 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of ccb^{dsRNA.UAS.cMa} and Rab11^UAS.EGFP under the control of Scer\GAL4^Toll-6-D42 leads to a decrease in the size of NMJ of larval motor neurons innervating muscle fibers 6 and 7.

(Martin-Peña and Ferrus, 2020)

The increased average amplitude of miniature (in case of Hsap\SNCA^Scer\UAS.cFa) or evoked excitatory junction potentials (in case of Hsap\SNCA^Scer\UAS.cKa) at neuromuscular junctions of third instar larvae as well as the abnormal frequency distribution of mEJP amplitudes seen in larvae expressing either Hsap\SNCA^Scer\UAS.cFa or Hsap\SNCA^Scer\UAS.cKa under the control of Scer\GAL4^elav-C155 can be restored by co-expression of Rab11^{Scer\UAS.T:Avic\GFP-EGFP}.

The increased mean size of synaptic vesicles as well as the shift in frequency distribution toward larger vesicles at both type 1b and 1s of neuromuscular junction boutons characteristic for third instar larvae expressing either Hsap\SNCA^Scer\UAS.cFa or Hsap\SNCA^Scer\UAS.cKa under the control of Scer\GAL4^elav-C155 can be suppressed by co-expression of Rab11^{Scer\UAS.T:Avic\GFP-EGFP}.

The defective crawling behavior (decrease in distance travelled per unit of time) characteristic for third instar larvae expressing Hsap\SNCA^Scer\UAS.cFa under the control of either Scer\GAL4^C164 or Scer\GAL4^ple.PF can be suppressed by co-expression of Rab11^{Scer\UAS.T:Avic\GFP-EGFP} using the Scer\GAL4^C164 driver and partially suppressed when the Scer\GAL4^ple.PF driver is used.

The defective crawling behavior (decrease in distance travelled per unit of time) characteristic for third instar larvae expressing Hsap\SNCA^Scer\UAS.cKa under the control of either Scer\GAL4^C164 or Scer\GAL4^ple.PF can be suppressed by co-expression of Rab11^{Scer\UAS.T:Avic\GFP-EGFP} using either of the two drivers.

The loss of dopaminergic PPL1 neurons in the adult brain characteristic for flies expressing Hsap\SNCA^Scer\UAS.cFa under the control of either Scer\GAL4^elav-C155 or Scer\GAL4^ple.PF can be reversed by co-expression of Rab11^{Scer\UAS.T:Avic\GFP-EGFP} using either of the two drivers.

The loss of dopaminergic PPL1 neurons in the adult brain characteristic for flies expressing Hsap\SNCA^Scer\UAS.cKa under the control of Scer\GAL4^elav-C155 can be reversed by co-expression of Rab11^{Scer\UAS.T:Avic\GFP-EGFP}.

The decrease in the number of rhabdomeres in the adult eye observed in flies expressing Hsap\SNCA^Scer\UAS.cFa under the control Scer\GAL4^GMR.PU can be partially restored by co-expression of Rab11^{Scer\UAS.T:Avic\GFP-EGFP}.

The defects in climbing behavior seen in adult flies or various ages (10, 20 and 30 day-old) expressing either Hsap\SNCA^Scer\UAS.cFa or Hsap\SNCA^Scer\UAS.cKa under the control of Scer\GAL4^elav-C155 can be suppressed by co-expression of Rab11^{Scer\UAS.T:Avic\GFP-EGFP}.

The defects in climbing behavior seen in adult flies or various ages (10, 20 and 30 day-old) expressing Hsap\SNCA^Scer\UAS.cFa under the control of Scer\GAL4^ple.PF are suppressed by co-expression of Rab11^{Scer\UAS.T:Avic\GFP-EGFP} in 30 day-old flies only.

The shortened lifespan characteristic for adult flies expressing either Hsap\SNCA^Scer\UAS.cFa or Hsap\SNCA^Scer\UAS.cKa under the control of Scer\GAL4^elav-C155 can be reversed by co-expression of Rab11^{Scer\UAS.T:Avic\GFP-EGFP}.

(Breda et al., 2015)

Xenogenetic Interactions

Statement

Reference

Co-expression of Rab11^{Scer\UAS.T:Avic\GFP-EGFP} almost completely rescues the wings defects caused by expression of Vcho\ctxA^Scer\UAS.cGa under the control of Scer\GAL4^Bx-MS1096.

Co-expression of Rab11^{Scer\UAS.T:Avic\GFP-EGFP} suppresses the emaciated, reduced body weight phenotype seen in flies expressing Vcho\ctxA^Scer\UAS.cGa under the control of Scer\GAL4^{Myo31DF-NP0001} in the presence of Scer\GAL80^ts.αTub84B at 22[o]C (a permissive temperature for Scer\GAL80^ts.αTub84B that reduces but does not eliminate Scer\GAL4^{Myo31DF-NP0001} activity).

Co-expression of Rab11^{Scer\UAS.T:Avic\GFP-EGFP} reduces the size of the gaps seen between midgut intestinal epithelial cells in flies expressing Vcho\ctxA^Scer\UAS.cGa under the control of Scer\GAL4^{Myo31DF-NP0001} during the adult stage (temperature sensitive Scer\GAL80^ts.αTub84B allele used to limit expression).

(Guichard et al., 2013)

Expression of Rab11^{Scer\UAS.T:Avic\GFP-EGFP} suppresses the reduction in average synaptic vesicle diameter seen when Hsap\HTT^{Q93.ex1p.Scer\UAS} is expressed under the control of Scer\GAL4^elav-C155.

Expression of Rab11^{Scer\UAS.T:Avic\GFP-EGFP} suppresses the reduction in eEJP and mEJP amplitudes seen when Hsap\HTT^{Q93.ex1p.Scer\UAS} is expressed under the control of Scer\GAL4^elav-C155 to levels comparable with controls. No significant difference is seen in mEJP decay kinetics, mEJP frequency, mean resting membrane potential or quantal content compared to controls.

Expression of Rab11^{Scer\UAS.T:Avic\GFP-EGFP} suppresses the third instar larval crawling defects seen when Hsap\HTT^{Q93.ex1p.Scer\UAS} is expressed under the control of Scer\GAL4^C164. The number of turns and distance travelled is comparable to controls.

(Steinert et al., 2012)

Overexpression of Rab11^{Scer\UAS.T:Avic\GFP-EGFP} almost completely suppresses the Hsap\HTT^{Q93.ex1p.Scer\UAS} eclosion phenotype, with a greater than twofold increase in emergence.

Overexpression of Rab11^{Scer\UAS.T:Avic\GFP-EGFP} gives a highly significant 43% suppression of the rhadomere degeneration seen upon expression of Hsap\HTT^{Q93.ex1p.Scer\UAS} under the control of Scer\GAL4^elav-C155.

Overexpression of Rab11^{Scer\UAS.T:Avic\GFP-EGFP} increases median survival of Hsap\HTT^{Q93.ex1p.Scer\UAS} by 50% from 10 to 15 days.

(Richards et al., 2011)

Expression of Rab11^{Scer\UAS.T:Avic\GFP-EGFP} in the wing disc primordium suppresses the activity of Zzzz\EF^Scer\UAS.cGa (when both are under the control of Scer\GAL4^Bx-MS1096).

(Guichard et al., 2010)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

8506

w^*; P{UAS-Rab11-GFP}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Rab11^{Scer\UAS.T:Avic\GFP-EGFP}

Rab11^UAS.EGFP

Name Synonyms

Secondary FlyBase IDs

References (31)

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