FlyBase Allele Report: Dmel\puc[E69]

The size of regenerated adult wings in puc^E69 heterozygous mutants that were subjected to ablation of imaginal wing disc tissue during the larval stage, is not reduced compared to similarly treated controls.

(Khan et al., 2017)

Heterozygous puc^E69 mutants display no perturbation to neuromuscular junction size.

(West et al., 2015)

puc^E69/+ flies are more resistant to oxidative stress (paraquat), have extended lifespans, and have significantly better climbing ability (at 40 days old, but not younger) than wild type.

(Huang et al., 2014)

puc^E69 heterozygotes exhibit a 25% frequency of ensheathment defects compared with only 11% in controls. puc^E69 homozygotes exhibit a 50% frequency of ensheathment defects.

(Jemc et al., 2012)

Wings of heterozygous puc^E69 flies appear normal.

(Neisch et al., 2010)

puc^E69/+ flies are more resistant against paraquat toxicity than controls.

(Wu et al., 2009)

40% of stage 17 homozygous embryos show inversion of the normal left-right asymmetry of the anterior midgut and 5% do not show any left-right asymmetry.

(Taniguchi et al., 2007)

Homozygotes have a larval cuticle with a puckered dorsal midline.

(Polaski et al., 2006)

puc^E69 heterozygous adults have signifcantly longer average and maximum life spans than wild-type.

(Wang et al., 2005)

In contrast to wild-type flies, puc^E69/+ flies do not die in response to being fed with paraquat. However, the sensitivity of these flies to G148 is similar to wild-type flies. puc^E69/+ males show significant extension of mean and maximal lifespan when compared to wild-type flies. Females show qualitatively similar lifespan data, although the extension of lifespan is less pronounced than in males. puc^E69/+ flies and wild-type controls exhibit roughly equivalent sizes, reproductive activities and developmental timing. However, these mutants show a much stronger resistance to starvation than wild-type flies, and also contain significantly decreased levels of oxidized proteins.

(Wang et al., 2003)

Heterozygous embryos do not show cuticle defects.

(Wei et al., 2003)

Dorsal closure takes place in homozygous embryos but the leading edge cells do not differentiate properly. The dorsal ectoderm closes properly, but the two rows of cardioblasts do not migrate and join to form the heart tube at the dorsal midline, instead remaining separate.

(Chartier et al., 2002)

Homozygous puc^E69 cells do not survive in the eye disc.

(Weber et al., 2000)

Heterozygotes have wild-type wings. The number of dying cells in the late third larval instar wing disc is not increased compared to wild type.

(Adachi-Yamada et al., 1999)

Mutant embryos show a severe crinkling of the dorsal epidermis and a small, posterior, dorsal hole. The fine dorsal hairs are disrupted.

(Zecchini et al., 1999)

Homozygous embryos lack dorsal hairs along the dorsal midline, leaving a strip of naked cuticle along most of the midline. These embryos show puckering of the epidermis.

(Martin-Blanco et al., 1998)

Dorsal closure proceeds normally in puc^E69 cno³ double heterozygotes.

(Takahashi et al., 1998)

Homozygous embryos produce a mutant cuticle phenotype in the dorsal epidermis. After germband shortening the cells are not restricted to a straight line at the edge of the epidermis, but are seen to extend several cell diameters away from the edge. As dorsal closure proceeds these cells become increasingly disorganised so that by completion of dorsal closure they are haphazardly arranged in clusters at the dorsal midline. The shape of the dorsal-most cells is also abnormal, the cells do not elongate and consequently they do not generate a straight edge to the moving front of the epidermis during dorsal closure. The pattern of the cuticle secreted by these cells is also abnormal. Despite the abnormalities of the dorsalmost epidermal cells, dorsal closure does occur to completion.

(Ring and Martinez Arias, 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Csk^j1D8, puc^E69/puc[+] has visible phenotype, enhanceable by ASPP[+]/ASPP⁸

(Langton et al., 2007)

NOT Enhanced by

Statement

Reference

puc^E69 has chemical resistant | dominant phenotype, non-enhanceable by ry⁵⁰⁶/ry⁵⁰⁶

(Wang et al., 2003)

Suppressed by

Statement

Reference

gfr¹, puc^E69/puc[+] has lethal | dominant phenotype, suppressible by bru3⁰⁵⁸⁷¹/l(3)05871[+]

(Beam and Moberg, 2010)

gfr¹, puc^E69/puc[+] has lethal | dominant phenotype, suppressible by Df(3L)Exel6119/+

(Beam and Moberg, 2010)

Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1, puc^E69/puc[+] has visible phenotype, suppressible by Rho1^72R/Rho1[+]

(Neisch et al., 2010)

Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1, puc^E69/puc[+] has visible phenotype, suppressible by hep^NIG.4353R, Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1, puc^E69/puc[+] has visible phenotype, suppressible by slpr^NIG.2272R, Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1, puc^E69/puc[+] has visible phenotype, suppressible by Tak1^NIG.1388R, Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1, puc^E69/puc[+] has visible phenotype, suppressible by Tak1^K46R.M.UAS, Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1, puc^E69/puc[+] has visible phenotype, suppressible by POSH^GD11467, Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

puc^E69 has abnormal oxidative stress response | dominant phenotype, suppressible by Atg1[+]/Atg1^Δ3D

(Wu et al., 2009)

puc^E69 has abnormal oxidative stress response | dominant phenotype, suppressible by Atg1^EY09216/Atg1[+]

(Wu et al., 2009)

puc^E69 has abnormal oxidative stress response | dominant phenotype, suppressible by Atg6[+]/Atg6⁰⁰⁰⁹⁶

(Wu et al., 2009)

puc^E69 has long lived | dominant phenotype, suppressible by foxo²⁵/foxo[+]

(Wang et al., 2005)

puc^E69 has long lived | dominant phenotype, suppressible by foxo²¹/foxo[+]

(Wang et al., 2005)

puc^E69 has chemical resistant | dominant phenotype, suppressible by bsk²/bsk[+]

(Wang et al., 2003)

puc^E69 has long lived | dominant phenotype, suppressible by hep¹

(Wang et al., 2003)

puc^E69 has chemical resistant | dominant phenotype, suppressible by hep¹

(Wang et al., 2003)

bi^omb-1, puc^E69 has increased cell death phenotype, suppressible by fu¹

(Adachi-Yamada and O'Connor, 2002)

puc^E69 has lethal | recessive phenotype, suppressible by hep¹

(Weber et al., 2000)

puc^E69 has lethal | recessive phenotype, suppressible by hep¹/hep¹

(Zeitlinger and Bohmann, 1999)

NOT suppressed by

Statement

Reference

gfr^x, puc^E69/puc[+] has lethal | dominant phenotype, non-suppressible by Df(3L)H99/+

(Beam and Moberg, 2010)

Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1, puc^E69/puc[+] has visible phenotype, non-suppressible by Ask1^K618M.UAS, Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

Enhancer of

Statement

Reference

puc^E69/puc[+] is an enhancer of neoplasia phenotype of Sox21a⁶

(Zhai et al., 2015)

puc^E69/puc[+] is an enhancer of abnormal cell migration phenotype of Scer\GAL4^ptc-559.1, Sin3A^KK100700

(Das et al., 2013)

puc^E69/puc[+] is an enhancer of increased cell death phenotype of Mmus\Gria1^Lc.UAS, Scer\GAL4^hs.2sev

(Yang et al., 2013)

BacA\p35^GMR.PH, puc^E69, puc[+] is an enhancer of increased cell death phenotype of BacA\p35^GMR.PH, Mmus\Gria1^Lc.UAS, Scer\GAL4^hs.2sev

(Yang et al., 2013)

puc^E69/puc[+], Scer\GAL4^dpp.blk1 is an enhancer of visible phenotype of Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

puc^E69/puc[+], Scer\GAL4^dpp.blk1 is an enhancer of visible phenotype of Rho1^UAS.cMa, Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

puc^E69/puc[+], Scer\GAL4^salm.EPv is an enhancer of visible phenotype of Axud1^UAS.cGa, Scer\GAL4^salm.EPv

(Glavic et al., 2009)

puc^E69/puc[+] is an enhancer of visible phenotype of Rala^35d

(Balakireva et al., 2006)

puc^E69/puc[+] is an enhancer of visible | dominant phenotype of sd^SG29.1

(Miotto et al., 2006)

puc^E69/puc[+] is an enhancer of visible phenotype of Scer\GAL4^pnr-MD237, slpr^UASp.cPa

(Polaski et al., 2006)

puc^E69/puc[+] is an enhancer of increased cell death phenotype of Csk^RNAi.UAS, Scer\GAL4^ptc-559.1

(Vidal et al., 2006)

puc^E69 is an enhancer of visible phenotype of H^UAS.cMa, Scer\GAL4^GMR.PF

(Müller et al., 2005)

puc^E69/puc[+] is an enhancer of abnormal cell growth | somatic clone phenotype of arr²

(Giraldez and Cohen, 2003)

puc^E69/puc[+] is an enhancer of increased cell death phenotype of Scer\GAL4^C-765, egr^UAS.cMa

(Moreno et al., 2002)

puc^E69/puc[+] is an enhancer of increased cell death phenotype of Scer\GAL4^GMR.PF, egr^UAS.cMa

(Moreno et al., 2002)

puc^E69/puc[+] is an enhancer of visible phenotype of Rac1^GMR.PN

(Stronach and Perrimon, 2002)

puc^E69/puc[+] is an enhancer of visible phenotype of dpp^hr56/dpp^d8

(Adachi-Yamada et al., 1999)

puc^E69/puc[+] is an enhancer of visible | recessive phenotype of bi^omb-1

(Adachi-Yamada et al., 1999)

puc^E69 is an enhancer of visible phenotype of tkv⁷/tkv⁴²⁷

(Adachi-Yamada et al., 1999)

puc^E69 is an enhancer of visible phenotype of Scer\GAL4^pnr-MD237, hep^UAS.cBa

(Zeitlinger and Bohmann, 1999)

Suppressor of

Statement

Reference

puc^E69 is a suppressor | partially of decreased rate of adult locomotory behavior | progressive phenotype of Ddrgk1^NIG.5862R, Scer\GAL4^elav.PU

(Li et al., 2023)

puc^E69 is a suppressor | partially of short lived phenotype of Ddrgk1^NIG.5862R, Scer\GAL4^elav.PU

(Li et al., 2023)

puc^E69/puc[+] is a suppressor of visible | adult stage phenotype of Scer\GAL4^pnr.PU, lic^GD7546

(Sun et al., 2019)

puc^E69/puc[+] is a suppressor of visible | adult stage phenotype of mol^e02670

(Khan et al., 2017)

puc^E69/puc[+] is a suppressor of abnormal stress response | adult stage phenotype of mol^e02670

(Khan et al., 2017)

puc^E69/puc[+] is a suppressor of short lived phenotype of Mrp4^M2/Mrp4^M1

(Huang et al., 2014)

puc^E69/puc[+] is a suppressor of abnormal locomotor behavior | progressive phenotype of Mrp4^M2/Mrp4^M1

(Huang et al., 2014)

puc^E69/puc[+] is a suppressor of abnormal oxidative stress response phenotype of Mrp4^M2

(Huang et al., 2014)

puc^E69/puc[+] is a suppressor of short lived | conditional phenotype of Hayan¹

(Nam et al., 2012)

puc^E69/puc[+] is a suppressor of partially lethal - majority die | larval stage | conditional phenotype of Hayan¹

(Nam et al., 2012)

puc^E69/puc[+] is a suppressor of lethal phenotype of slpr^BS06

(Baril et al., 2009)

puc^E69/puc[+] is a suppressor of visible | male phenotype of JMJD6^UAS.cKa, Scer\GAL4^Act5C.PU

(Krieser et al., 2007)

puc^E69 is a suppressor of visible phenotype of Rala^G20V.cMa.UAS, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

puc^E69/puc[+] is a suppressor of partially lethal - majority die phenotype of slpr^BS06

(Polaski et al., 2006)

puc^E69/puc[+] is a suppressor of visible phenotype of Scer\GAL4^pnr-MD237, slpr^D314Y.UASp

(Polaski et al., 2006)

puc^E69/puc[+] is a suppressor | partially of abnormal immune response phenotype of Irc^RNAi.UAS, Scer\GAL4^da.G32

(Ha et al., 2005)

puc^E69/puc[+] is a suppressor of chemical sensitive | dominant phenotype of bsk²

(Wang et al., 2003)

puc^E69/puc[+] is a suppressor of chemical sensitive phenotype of hep¹

(Wang et al., 2003)

puc^E69 is a suppressor | partially of lethal phenotype of kay¹/kay²

(Ramet et al., 2002)

puc^E69 is a suppressor of abnormal neuroanatomy phenotype of Jra^Jbz.UAS, Scer\GAL4^elav-C155

(Sanyal et al., 2002)

puc^E69 is a suppressor of abnormal neurophysiology phenotype of Jra^Jbz.UAS, Scer\GAL4^elav-C155

(Sanyal et al., 2002)

puc^E69/puc[+] is a suppressor of lethal | recessive phenotype of Src42A^Jp45

(Tateno et al., 2000)

puc^E69 is a suppressor of visible phenotype of hep^r39/hep¹

(Agnes et al., 1999)

puc^E69 is a suppressor of visible phenotype of hep^r75/hep¹

(Agnes et al., 1999)

puc^E69/puc[+] is a suppressor of lethal phenotype of kay¹/kay²

(Zeitlinger and Bohmann, 1999)

puc^E69/puc[+] is a suppressor of lethal | recessive | maternal effect | embryonic stage phenotype of hep¹

(Zeitlinger and Bohmann, 1999)

NOT Suppressor of

Statement

Reference

puc^E69 is a non-suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^elav-C155, kay^Fbz.UAS

(Sanyal et al., 2002)

puc^E69 is a non-suppressor of abnormal neurophysiology phenotype of Scer\GAL4^elav-C155, kay^Fbz.UAS

(Sanyal et al., 2002)

Other

Statement

Reference

Rab8¹/Rab8[+], puc^E69 has abnormal neuroanatomy phenotype

(West et al., 2015)

Df(3L)H99/+, puc^E69 has viable phenotype

(Beam and Moberg, 2010)

bru3⁰⁵⁸⁷¹/l(3)05871[+], puc^E69 has viable phenotype

(Beam and Moberg, 2010)

cic^Q474X, puc^E69/puc[+] has partially lethal - majority die | dominant phenotype

(Beam and Moberg, 2010)

gfr¹, puc^E69/puc[+] has lethal | dominant phenotype

(Beam and Moberg, 2010)

bru3⁰⁵⁸⁷¹/l(3)05871[+], gfr¹, puc^E69/puc[+] has viable phenotype

(Beam and Moberg, 2010)

Df(3L)Exel6119/+, gfr¹, puc^E69/puc[+] has viable phenotype

(Beam and Moberg, 2010)

gfr², puc^E69/puc[+] has lethal | dominant phenotype

(Beam and Moberg, 2010)

gfr^x, puc^E69/puc[+] has lethal | dominant phenotype

(Beam and Moberg, 2010)

Csk^j1D8, puc^E69/puc[+] has increased cell death phenotype

(Langton et al., 2007)

Csk^j1D8, puc^E69/puc[+] has visible phenotype

(Langton et al., 2007)

ASPP⁸, puc^E69/puc[+] has increased cell death | third instar larval stage phenotype

(Langton et al., 2007)

ASPP[+]/ASPP⁸, Csk^j1D8, puc^E69/puc[+] has visible phenotype

(Langton et al., 2007)

chm¹⁴/chm[+], puc^E69 has viable phenotype

(Miotto et al., 2006)

chm¹⁴, puc^E69/puc[+] has viable phenotype

(Miotto et al., 2006)

bi^omb-1, puc^E69 has increased cell death phenotype

(Adachi-Yamada and O'Connor, 2002)

puc^E69/puc[+], slpr^3P5 has partially lethal phenotype

(Stronach and Perrimon, 2002)

hep¹, puc^E69 has visible phenotype

(Weber et al., 2000)

hep¹, puc^E69 has abnormal planar polarity phenotype

(Weber et al., 2000)

hep¹, puc^E69/puc[+] has viable phenotype

(Zeitlinger and Bohmann, 1999)

hep¹, puc^E69 has viable phenotype

(Zeitlinger and Bohmann, 1999)

kay¹/kay², puc^E69 has viable phenotype

(Zeitlinger and Bohmann, 1999)

Phenotype Manifest In

Enhanced by

Statement

Reference

Csk^j1D8, puc^E69/puc[+] has wing phenotype, enhanceable by ASPP[+]/ASPP⁸

(Langton et al., 2007)

Suppressed by

Statement

Reference

Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1, puc^E69/puc[+] has wing blade phenotype, suppressible by Rho1^72R/Rho1[+], Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1, puc^E69/puc[+] has wing blade phenotype, suppressible by hep^NIG.4353R, Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1, puc^E69/puc[+] has wing blade phenotype, suppressible by slpr^NIG.2272R, Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1, puc^E69/puc[+] has wing blade phenotype, suppressible by Tak1^NIG.1388R, Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1, puc^E69/puc[+] has wing blade phenotype, suppressible by Tak1^K46R.M.UAS, Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1, puc^E69/puc[+] has wing blade phenotype, suppressible by POSH^GD11467, Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

Scer\GAL4^GMR.PF, egr^UAS.cIa, puc^E69/puc[+] has eye phenotype, suppressible by lncRNA:Hsrω^RNAi.Sym.UAS/Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2009)

bi^omb-1, puc^E69 has wing phenotype, suppressible by fu¹

(Adachi-Yamada and O'Connor, 2002)

puc^E69/puc[+], tkv⁷/tkv⁴²⁷ has wing disc phenotype, suppressible by hep^r75

(Adachi-Yamada et al., 1999)

puc^E69, tkv⁷/tkv⁴²⁷ has wing disc phenotype, suppressible by hep^r75

(Adachi-Yamada et al., 1999)

NOT suppressed by

Statement

Reference

Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1, puc^E69/puc[+] has wing blade phenotype, non-suppressible by Ask1^K618M.UAS, Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

Enhancer of

Statement

Reference

puc^E69/puc[+] is an enhancer of adult anterior midgut epithelium phenotype of Sox21a⁶

(Zhai et al., 2015)

puc^E69/puc[+] is an enhancer of wing disc phenotype of Scer\GAL4^ptc-559.1, Sin3A^KK100700

(Das et al., 2013)

puc^E69/puc[+] is an enhancer of eye phenotype of Mmus\Gria1^Lc.UAS, Scer\GAL4^hs.2sev

(Yang et al., 2013)

Scer\GAL4^hs.2sev, BacA\p35^GMR.PH, puc^E69, puc[+] is an enhancer of eye phenotype of BacA\p35^GMR.PH, Mmus\Gria1^Lc.UAS, Scer\GAL4^hs.2sev

(Yang et al., 2013)

BacA\p35^GMR.PH, puc^E69, puc[+], Scer\GAL4^hs.2sev is an enhancer of eye phenotype of Mmus\Gria1^Lc.UAS, Scer\GAL4^hs.2sev

(Yang et al., 2013)

puc^E69/puc[+] is an enhancer of eye | somatic clone phenotype of ebi^k16213

(Lim et al., 2012)

puc^E69/puc[+], Scer\GAL4^dpp.blk1 is an enhancer of wing blade phenotype of Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

puc^E69/puc[+], Scer\GAL4^dpp.blk1 is an enhancer of wing blade phenotype of Rho1^UAS.cMa, Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

puc^E69/puc[+] is an enhancer of adult thorax phenotype of Scer\GAL4^pnr-MD237, slpr^UASp.cPa

(Baril et al., 2009)

puc^E69/puc[+] is an enhancer of scutellar bristle phenotype of Scer\GAL4^pnr-MD237, slpr^UASp.cPa

(Baril et al., 2009)

puc^E69/puc[+], Scer\GAL4^salm.EPv is an enhancer of wing blade phenotype of Axud1^UAS.cGa, Scer\GAL4^salm.EPv

(Glavic et al., 2009)

puc^E69/puc[+], Scer\GAL4^salm.EPv is an enhancer of wing phenotype of Axud1^UAS.cGa, Scer\GAL4^salm.EPv

(Glavic et al., 2009)

puc^E69/puc[+] is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, egr^UAS.cIa

(Mallik and Lakhotia, 2009)

puc^E69/puc[+] is an enhancer of scutellum phenotype of Scer\GAL4^pnr-MD237, slpr^UASp.cPa

(Baril et al., 2009, Polaski et al., 2006)

puc^E69/puc[+] is an enhancer of eye phenotype of Rab11^N124I.UAS, Scer\GAL4^GMR.PF

(Tiwari and Roy, 2009)

puc^E69/puc[+] is an enhancer of microchaeta phenotype of Rala^35d

(Balakireva et al., 2006)

puc^E69/puc[+] is an enhancer of macrochaeta phenotype of Rala^35d

(Balakireva et al., 2006)

puc^E69/puc[+], Scer\GAL4^Bx-MS1096 is an enhancer of wing phenotype of Lkb1^UAS.cLa, Scer\GAL4^Bx-MS1096

(Lee et al., 2006)

puc^E69/puc[+] is an enhancer of zonula adherens & disc epithelium proper phenotype of Csk^RNAi.UAS, Scer\GAL4^ptc-559.1

(Vidal et al., 2006)

puc^E69 is an enhancer of eye phenotype of H^UAS.cMa, Scer\GAL4^GMR.PF

(Müller et al., 2005)

puc^E69/puc[+] is an enhancer of wing phenotype of Scer\GAL4^C-765, egr^UAS.cMa

(Moreno et al., 2002)

puc^E69/puc[+] is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, egr^UAS.cMa

(Moreno et al., 2002)

puc^E69/puc[+] is an enhancer of eye phenotype of Rac1^GMR.PN

(Stronach and Perrimon, 2002)

puc^E69/puc[+] is an enhancer of ommatidium phenotype of Rac1^GMR.PN

(Stronach and Perrimon, 2002)

puc^E69/puc[+] is an enhancer of wing phenotype of dpp^hr56/dpp^d8

(Adachi-Yamada et al., 1999)

puc^E69 is an enhancer of wing phenotype of tkv⁷/tkv⁴²⁷

(Adachi-Yamada et al., 1999)

puc^E69 is an enhancer of wing disc phenotype of tkv⁷/tkv⁴²⁷

(Adachi-Yamada et al., 1999)

puc^E69/puc[+] is an enhancer of wing phenotype of bi^omb-1

(Adachi-Yamada et al., 1999)

puc^E69 is an enhancer of scutellum phenotype of Scer\GAL4^pnr-MD237, hep^UAS.cBa

(Zeitlinger and Bohmann, 1999)

NOT Enhancer of

Statement

Reference

puc^E69 is a non-enhancer of phenotype of Rho1^rev220

(Magie et al., 1999)

Suppressor of

Statement

Reference

puc^E69/puc[+] is a suppressor of thoracic tergum phenotype of Scer\GAL4^pnr.PU, lic^GD7546

(Sun et al., 2019)

puc^E69/puc[+] is a suppressor of wing phenotype of mol^e02670

(Khan et al., 2017)

puc^E69/puc[+] is a suppressor of male genitalia phenotype of JMJD6^UAS.cKa, Scer\GAL4^Act5C.PU

(Krieser et al., 2007)

puc^E69 is a suppressor of macrochaeta phenotype of Rala^G20V.cMa.UAS, Scer\GAL4^sca-537.4

(Balakireva et al., 2006)

puc^E69/puc[+] is a suppressor of embryonic/first instar larval cuticle | dorsal | germline clone phenotype of Dok^PG155

(Biswas et al., 2006)

puc^E69/puc[+] is a suppressor of adult thorax | pharate adult stage phenotype of chm¹⁴

(Miotto et al., 2006)

puc^E69/puc[+] is a suppressor of mesothoracic tergum phenotype of Scer\GAL4^pnr-MD237, slpr^D314Y.UASp

(Polaski et al., 2006)

puc^E69 is a suppressor | partially of mesothoracic tergum phenotype of Pvr^RNAi.UAS, Scer\GAL4^pnr-MD237

(Ishimaru et al., 2004)

puc^E69 is a suppressor of thorax phenotype of Pvr^RNAi.UAS, Scer\GAL4^pnr-MD237

(Ishimaru et al., 2004)

puc^E69 is a suppressor of embryo | dorsal closure stage phenotype of Scer\GAL4^pnr-MD237, knrl^UAS.cLa

(Gritzan et al., 2002)

puc^E69 is a suppressor | partially of mesothoracic cleft phenotype of kay¹/kay²

(Ramet et al., 2002)

puc^E69 is a suppressor of bouton phenotype of Jra^Jbz.UAS, Scer\GAL4^elav-C155

(Sanyal et al., 2002)

puc^E69 is a suppressor of neuromuscular junction | larval stage phenotype of Jra^Jbz.UAS, Scer\GAL4^elav-C155

(Sanyal et al., 2002)

puc^E69/puc[+] is a suppressor of phenotype of slpr^3P5

(Stronach and Perrimon, 2002)

puc^E69/puc[+] is a suppressor of embryo | dorsal closure stage phenotype of slpr⁹²¹

(Stronach and Perrimon, 2002)

puc^E69/puc[+] is a suppressor of embryonic/first instar larval cuticle phenotype of slpr⁹²¹

(Stronach and Perrimon, 2002)

puc^E69/puc[+] is a suppressor of adult thorax | dorsal phenotype of Src42A^Jp45

(Tateno et al., 2000)

puc^E69 is a suppressor of adult thorax phenotype of hep^r39/hep¹

(Agnes et al., 1999)

puc^E69 is a suppressor of chaeta phenotype of hep^r39/hep¹

(Agnes et al., 1999)

puc^E69 is a suppressor of adult thorax phenotype of hep^r75/hep¹

(Agnes et al., 1999)

puc^E69 is a suppressor of chaeta phenotype of hep^r75/hep¹

(Agnes et al., 1999)

puc^E69 is a suppressor of wing phenotype of hep¹

(Agnes et al., 1999)

puc^E69 is a suppressor of leg phenotype of hep¹

(Agnes et al., 1999)

puc^E69 is a suppressor of eye phenotype of hep¹

(Agnes et al., 1999)

puc^E69 is a suppressor of wing disc phenotype of hep¹

(Agnes et al., 1999)

puc^E69 is a suppressor of imaginal disc phenotype of hep^r39

(Agnes et al., 1999)

puc^E69 is a suppressor of wing disc phenotype of hep^r39

(Agnes et al., 1999)

puc^E69 is a suppressor of imaginal disc phenotype of hep^r75

(Agnes et al., 1999)

puc^E69 is a suppressor of wing disc phenotype of hep^r75

(Agnes et al., 1999)

puc^E69 is a suppressor of adult thorax | dorsal phenotype of kay²

(Zeitlinger and Bohmann, 1999)

puc^E69 is a suppressor of embryonic/first instar larval cuticle | dorsal phenotype of hep¹

(Martin-Blanco et al., 1998)

NOT Suppressor of

Statement

Reference

puc^E69 is a non-suppressor of bouton phenotype of Scer\GAL4^elav-C155, kay^Fbz.UAS

(Sanyal et al., 2002)

puc^E69 is a non-suppressor of neuromuscular junction | larval stage phenotype of Scer\GAL4^elav-C155, kay^Fbz.UAS

(Sanyal et al., 2002)

puc^E69/puc[+] is a non-suppressor of embryonic epidermis | ventral phenotype of arm³

(McEwen et al., 2000)

puc^E69/puc[+] is a non-suppressor of ventral denticle belt phenotype of arm³

(McEwen et al., 2000)

puc^E69/puc[+] is a non-suppressor of embryonic epidermis | dorsal phenotype of arm³

(McEwen et al., 2000)

puc^E69 is a non-suppressor of phenotype of Rho1^rev220

(Magie et al., 1999)

Other

Statement

Reference

Rab8¹/Rab8[+], puc^E69 has NMJ bouton | third instar larval stage phenotype

(West et al., 2015)

Rab8¹/Rab8[+], puc^E69 has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(West et al., 2015)

Csk^j1D8, puc^E69/puc[+] has wing phenotype

(Langton et al., 2007)

Csk^j1D8, puc^E69/puc[+] has wing pouch phenotype

(Langton et al., 2007)

ASPP⁸, puc^E69/puc[+] has wing disc phenotype

(Langton et al., 2007)

ASPP[+]/ASPP⁸, Csk^j1D8, puc^E69/puc[+] has leg phenotype

(Langton et al., 2007)

Sac1[+]/Sac1^L2F, puc^E69 has embryonic/first instar larval cuticle phenotype

(Wei et al., 2003)

Sac1^L2F, puc^E69 has embryonic/first instar larval cuticle phenotype

(Wei et al., 2003)

bi^omb-1, puc^E69 has wing phenotype

(Adachi-Yamada and O'Connor, 2002)

hep¹, puc^E69 has eye phenotype

(Weber et al., 2000)

hep¹, puc^E69 has ommatidium phenotype

(Weber et al., 2000)

hep¹, puc^E69 has macrochaeta phenotype

(Zeitlinger and Bohmann, 1999)

kay¹/kay², puc^E69 has adult thorax | dorsal phenotype

(Zeitlinger and Bohmann, 1999)

Additional Comments

Genetic Interactions

Statement

Reference

The reduced size of regenerated adult wings characteristic for mol^e02670 heterozygous mutants that were subjected to ablation of the imaginal wing disc tissue during the larval stage can be rescued by combination with a single copy of puc^E69.

(Khan et al., 2017)

Heterozygous combinations of puc^E69 and Rab8¹ results in an increase in synaptic bouton number compared with wild-type.

(West et al., 2015)

One copy of puc^E69 increases tumor formation in the anterior midgut of Sox21a⁶ mutant flies.

(Zhai et al., 2015)

Reduced resistance to oxidative stress (paraquat) in Mrp4^M2/Mrp4^M2 flies is increased to significantly more than wild type levels with a single copy of puc^E69. Reduced lifespan in Mrp4^M1/Mrp4^M2 flies is increased to significantly more than wild type levels with a single copy of puc^E69. Reduced climbing ability in Mrp4^M1/Mrp4^M2 flies is rescued to wild type levels (at 10 and 30 days old) and increased beyond wild type levels (at 40 days old) by a single copy of puc^E69.

(Huang et al., 2014)

The cell invasion phenotype seen when Sin3A^KK100700 is expressed under the control of Scer\GAL4^ptc-559.1 is enhanced in a puc^E69/+ background.

(Das et al., 2013)

puc^E69/+ enhances the mild rough eye phenotype seen in eyes containing large ebi^k16213 clones.

(Lim et al., 2012)

A puc^E69/+ mutant background suppresses the mortality induced when homozygous Hayan¹ mutants are wounded at either the larval or adult stages.

(Nam et al., 2012)

puc^E69 dominantly suppresses the wing notching phenotype seen in Df(1)N-54l9 heterozygotes (the penetrance of the phenotype is significantly decreased in the double heterozygotes).

(Beam and Moberg, 2010)

Heterozygous puc^E69 mutant flies expressing Moe^{dsRNA.327-775.Scer\UAS} under the control of Scer\GAL4^dpp.blk1 exhibit a severely reduced or rudimentary wing phenotype.

Rho1^72R/+ strongly suppresses the Scer\GAL4^dpp.blk1>Moe^{dsRNA.327-775.Scer\UAS}; puc^E69/+ wing blade phenotype.

Co-expression of hep^{dsRNA.Scer\UAS} suppresses the Scer\GAL4^dpp.blk1>Moe^{dsRNA.327-775.Scer\UAS}; puc^E69/+ wing blade phenotype.

Co-expression of slpr^{dsRNA.Scer\UAS.NIG} suppresses the Scer\GAL4^dpp.blk1>Moe^{dsRNA.327-775.Scer\UAS}; puc^E69/+ wing blade phenotype.

Co-expression of Tak1^{dsRNA.Scer\UAS} suppresses the Scer\GAL4^dpp.blk1>Moe^{dsRNA.327-775.Scer\UAS}; puc^E69/+ wing blade phenotype.

Co-expression of Tak1^{K46R.M.Scer\UAS} suppresses the Scer\GAL4^dpp.blk1>Moe^{dsRNA.327-775.Scer\UAS}; puc^E69/+ wing blade phenotype.

Co-expression of Pk92B^{K618M.Scer\UAS} has no effect on the Scer\GAL4^dpp.blk1>Moe^{dsRNA.327-775.Scer\UAS}; puc^E69/+ wing blade phenotype.

Heterozygous puc^E69 mutant flies expressing Rho1^Scer\UAS.cMa under the control of Scer\GAL4^dpp.blk1 exhibit a severely reduced or rudimentary wing phenotype.

(Neisch et al., 2010)

puc^E69 restores viability to slpr^BS06/Y males.

puc^E69/+ strongly enhances the thorax closure defects of Scer\GAL4^pnr-MD237 slpr^{Scer\UAS.P\T.cPa} flies - the scutellum is smaller, and more bristles are lost.

(Baril et al., 2009)

Expression of Axud1^Scer\UAS.cGa with Scer\GAL4^salm-EPv in a heterozygous puc^E69 background significantly enhances the reduction in wing size.

(Glavic et al., 2009)

Introduction of a single copy of puc^E69 into the Scer\GAL4^GMR.PF, egr^Scer\UAS.cIa background dramatically enhances the reduced eye phenotype, with flies displaying a prominent black lesion in place of the eye.

(Mallik and Lakhotia, 2009)

The reduced eye phenotype caused by expression of Rab11^{N124I.Scer\UAS} under the control of Scer\GAL4^GMR.PF is enhanced if the flies also carry puc^E69/+.

(Tiwari and Roy, 2009)

Heterozygosity for Atg1^Δ3D, Atg1^EY09216 or Atg6⁰⁰⁰⁹⁶ reverts the resistance to paraquat toxicity seen in puc^E69/+ animals.

(Wu et al., 2009)

The genital rotation defect seen in males expressing PSR^Scer\UAS.cKa under the control of either Scer\GAL4^Act5C is suppressed by puc^E69/+.

(Krieser et al., 2007)

puc^E69/+ Csk^j1D8/+ double heterozygotes have a notched wing phenotype, and third larval instar wing discs show ectopic apoptosis within the wing pouch.

The ectopic apoptosis seen in the wing discs of puc^E69/+ Csk^j1D8/+ double heterozygotes is enhanced if they are also heterozygous for ASPP⁸, resulting in adults with a severely reduced wing size. These adults also have leg deformities.

The wing discs of ASPP⁸/ASPP⁸ puc^E69/+ third instar larvae show a moderate ectopic patch of apoptotic cells. Adults of this genotype do not show wing notching.

(Langton et al., 2007)

Only 20% of embryos derived from Dok^PG155 germ-line clones that are also heterozygous for puc^E69 show a dorsal closure phenotype, compared to 48% of Dok^PG155 germ-line clones without a puc mutation. The rescued embryos have small holes in the cuticle.

(Biswas et al., 2006)

A puc^E69/+ background dramatically enhances the lkb1^Scer\UAS.cLa-Scer\GAL4^Bx-MS1096 small wing phenotype.

(Lee et al., 2006)

The severity of the thoracic cleft seen in chm¹⁴ pharate adults is suppressed by heterozygosity for puc^E69.

(Miotto et al., 2006)

The narrowed scutellum phenotype seen in flies expressing slpr^{Scer\UAS.P\T.cPa} under the control of Scer\GAL4^pnr-MD237 is enhanced by puc^E69/+, such that the scutellum is essentially eliminated.

The cleft notum phenotype seen in flies expressing slpr^{D314Y.Scer\UAS.P\T} under the control of Scer\GAL4^pnr-MD237 is suppressed almost to wild type by puc^E69/+.

(Polaski et al., 2006)

puc^E69/+ strongly enhances the loss of apical profile, delamination and subsequent migration and cell death increase seen in cells at the posterior edge of theptc expression domain in Csk^IR.Scer\UAS Scer\GAL4^ptc-559.1 third instar larvae.

(Vidal et al., 2006)

The reduced survival rate after natural infection with "Ecc-15" (P.carotovorum.carotovorum) that is seen in flies expressing Irc^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 is partially rescued by puc^E69/+.

(Ha et al., 2005)

The increase in average and maximum lifespan seen in puc^E69/+ adults is dominantly suppressed by foxo²¹ or foxo²⁵.

(Wang et al., 2005)

Recovery of arr² homozygous somatic clones in third instar wing discs (induced at around 60 hours after egg laying) is reduced by the presence of puc^E69/+ (10/25 twinspots vs 21/29 in a wild-type background).

(Giraldez and Cohen, 2003)

puc^E69/+; hep¹/Y mutant flies have a decreased mortality rate in response to paraquat compared to hep¹/Y single mutants, but an increased mortality rate compared to puc^E69/+ single mutants. Likewise, puc^E69/+; bsk²/+ have a decreased sensitivity to paraquat compared to bsk²/+ single mutants and an increased mortality rate compared to puc^E69/+ single mutants. The response of puc^E69/+ flies to paraquat is not affected when flies have a ry⁵⁰⁶ genetic background. puc^E69/+ flies with a hep¹/Y background have a less pronounced extension of lifespan than puc^E69/+ single mutant flies.

(Wang et al., 2003)

puc^E69 Sac1^L2F double heterozygous embryos show defects in the dorsal cuticle (anterior head holes and/or puckering). puc^E69 shows no genetic interactions with Sac1²¹⁰⁷ or Sac1^BG02228. Sac1^L16C interacts genetically with puc^E69.

(Wei et al., 2003)

bi^omb-1, puc^E69 double heterozygotes exhibit a strong wing notch and cell death increase phenotype. These phenotypes are suppressed by the addition of fu¹.

(Adachi-Yamada and O'Connor, 2002)

The dorsal closure phenotype caused by expression of eg^Scer\UAS.cDa under the control of Scer\GAL4^pnr-MD237 is suppressed by puc^E69. The dorsal closure phenotype caused by expression of knrl^Scer\UAS.cLa under the control of Scer\GAL4^pnr-MD237 is suppressed by puc^E69.

(Gritzan et al., 2002)

Heterozygosity for puc^E69 enhances the wing phenotype due to P{UAS-eiger.M}weak with Scer\GAL4^C-765. puc^E69/+ enhances the eye ablation phentoype of egr^Scer\UAS.cMa; Scer\GAL4^GMR.PF animals.

(Moreno et al., 2002)

The severe dorsal open phenotype of slpr⁹²¹/Y embryos is significantly rescued by puc^E69/+. puc^E69/+ rescues slpr^3P5/Y animals to adulthood. The mutant male flies are weakly viable with no gross morphological defects.

(Stronach and Perrimon, 2002)

Dominantly suppresses the lethality and cleft thorax phenotypes of Src42A^Jp45 homozygotes.

(Tateno et al., 2000)

Homozygous escapers show mildly roughened eyes that display typical planar polarity defects.

(Weber et al., 2000)

Dominantly enhances the wing notching phenotype of dpp^d8/dpp^hr56, tkv⁷/tkv⁴²⁷ or bi^omb-1/Y flies. Dominantly enhances the appearance of an apoptotic cluster of cells in the primordial wing tip of the late third larval instar tkv⁷/tkv⁴²⁷ wing disc. The appearance of the cluster of apoptotic cells is suppressed in hep^r75/Y ; tkv⁷/tkv⁴²⁷; puc^E69/+ larvae.

(Adachi-Yamada et al., 1999)

The lethality of the kay¹/kay² combination is rescued by one copy of puc^E69; the rescued flies have a thoracic cleft phenotype ranging from strong to very mild. Rescues the thoracic cleft phenotype of kay² homozygotes. The lethality of embryos which are maternally and zygotically hep¹ is rescued by one copy of puc^E69. The lethality of puc^E69 homozygotes is rescued if the flies are also homozygous or hemizygous for hep¹. The rescued flies look remarkably normal, except some females have macrochaetae with a kink. Dominantly enhances the scutellar phenotype caused by hep^Scer\UAS.cBa expressed under the control of Scer\GAL4^pnr-MD237.

(Zeitlinger and Bohmann, 1999)

Dominantly partially suppresses the dorsal open phenotype of hep¹ hemizygous embryos.

(Martin-Blanco et al., 1998)

Xenogenetic Interactions

Statement

Reference

A puc^E69/+ background increases the severity the reduced adult eye size phenotype found in Scer\GAL4^hs.2sev->Mmus\Gria1^Lc.Scer\UAS flies.

A puc^E69/+ background further enhances the rescue found in BacA\p35^GMR.PH;Scer\GAL4^hs.2sev->Mmus\Gria1^Lc.Scer\UAS flies.

(Yang et al., 2013)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

98329

P{A92}puc^E69/TM3, Sb¹

Kyoto

109029

w^*; cno³ P{A92}puc^E69 / TM6B,abdA-LacZ

Notes on Origin

Discoverer

Comments

Reversion to wild-type is associated with loss of the P{A92} element. JNK activity is increased twofold compared to wild-type and ERK activity is similar to wild-type in extracts of homozygous embryos.

(Martin-Blanco et al., 1998)

Loss of the P{A92} insertion results in reversion of the puc phenotype to wild type.

(Ring and Martinez Arias, 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Puc^E69

(Li et al., 2023, Calderon et al., 2022, Zhou and Boutros, 2020, Wang et al., 2019, Vidal et al., 2006, Ha et al., 2005)

puc ^E69

(Zhang et al., 2010)

puc-LacZ

(Lee et al., 2006)

puc-lacZ

(Tsuda et al., 2006)

puc^E69

(Deshpande et al., 2023, Yang et al., 2022, Kong et al., 2021, Wada et al., 2021, Wu et al., 2021, Zhou et al., 2021, Irwin et al., 2020, Ma et al., 2020, Ugbode et al., 2020, Sun et al., 2019, Li et al., 2018, Khan et al., 2017, Ma et al., 2017, Ren et al., 2017, Tang et al., 2017, Zhang et al., 2016, Shklover et al., 2015, West et al., 2015, Zhai et al., 2015, Huang et al., 2014, Pérez-Garijo et al., 2013, Yang et al., 2013, Lim et al., 2012, Nam et al., 2012, Milton et al., 2011, Beam and Moberg, 2010, Neisch et al., 2010, Baril et al., 2009, Glavic et al., 2009, Mallik and Lakhotia, 2009, Tiwari and Roy, 2009, Tuxworth et al., 2009, Wu et al., 2009, Taniguchi et al., 2007, Balakireva et al., 2006, Lee et al., 2006, Polaski et al., 2006, Singh et al., 2006, Tsuda et al., 2006, Pastor-Pareja et al., 2004, Ryoo et al., 2004)

puc^E69lacZ

(Krieser et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (93)

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