FB2024_03 , released June 25, 2024
Gene: Dmel\eIF4G2
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General Information
Symbol
Dmel\eIF4G2
Species
D. melanogaster
Name
eukaryotic translation initiation factor 4G2
Annotation Symbol
CG10192
Feature Type
protein_coding_gene
FlyBase ID
FBgn0260634
Gene Model Status
Current
Stock Availability
7 publicly available
Gene Summary
eukaryotic translation initiation factor 4G2 (eIF4G2) encodes an eIF4G homolog involved in regulation of translation initiation within male germ cells. It is required for spermatogenesis. [Date last reviewed: 2019-09-12] (FlyBase Gene Snapshot)
All Summaries
Also Known As

ofs, off-schedule, eIF4G-2, nc32

Key Links
Genomic Location
Cytogenetic map
95B9-95C1
Sequence location
Recombination map
3-81
RefSeq locus
NT_033777 REGION:23808285..23819237
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (8 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables RNA 7-methylguanosine cap binding
inferred from direct assay
(Franklin-Dumont et al., 2007)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
enables mRNA binding
inferred from biological aspect of ancestor with PANTHER:PTN000583430
(GO Reference Genome Project, 2011-)
enables RNA binding
inferred from electronic annotation with InterPro:IPR003890
(InterPro Project Members, 2004-)
enables translation initiation factor activity
inferred from biological aspect of ancestor with PANTHER:PTN000583430
(GO Reference Genome Project, 2011-)
inferred from electronic annotation with InterPro:IPR045208
(InterPro Project Members, 2004-)
Biological Process (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
involved_in male meiotic nuclear division
inferred from mutant phenotype
(Baker and Fuller, 2007)
involved_in spermatogenesis
inferred from mutant phenotype
(Baker and Fuller, 2007)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in translational initiation
inferred from biological aspect of ancestor with PANTHER:PTN008594891
(GO Reference Genome Project, 2011-)
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
part_of eukaryotic translation initiation factor 4F complex
inferred from biological aspect of ancestor with PANTHER:PTN000583430
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
eukaryotic translation initiation factor 4G2 (eIF4G2) encodes an eIF4G homolog involved in regulation of translation initiation within male germ cells. It is required for spermatogenesis. [Date last reviewed: 2019-09-12]
Gene Group (FlyBase)
CYTOPLASMIC TRANSLATION INITIATION FACTORS -
Cytoplasmic (or eukaryotic) translation initiation factors function in the initiation stage of ribosome-mediated translation in the cytoplasm. (Adapted from FBrf0233552 and PMID:20094052).
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
ms(3)nc32
Fails to complement certain β Tub85D mutant alleles for male fertility although mapping to another site; β Tub85D synthesis, meiosis, and spermatid differentiation are normal in these sterile trans-heterozygotes. ms(3)nc32/ms(3)nc32 males fail to enter meiosis and show no spermatid differentiation. Testes from these males contain gonial cells, primary spermatocyte cysts, and cysts with abnormal cells. Adult male homozygotes show no β Tub85D synthesis. ms(3)nc32/+ males fertile.
Summary (Interactive Fly)

functions as translation initiation factor eIF4G during spermatogenesis to coordinate the initiation of meiotic division and differentiation

Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\eIF4G2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VCH1)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Shares 5' UTR with downstream gene.

Gene model reviewed during 5.53

Gene model reviewed during 5.47

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 6.04

Sequence Ontology: Class of Gene
gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
eIF4G2-RA
FBtr0089623
NM_142931
7297
2072
eIF4G2-RC
FBtr0344335
NM_001300560
8889
2072
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
eIF4G2-PA
FBpp0088566
226.6
2072
8.10
Q9VCH1
NP_651188
AAF56194
eIF4G2-PC
FBpp0310717
226.6
2072
8.10
Q9VCH1
NP_001287489
AHN57488
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

2072 aa isoforms: eIF4G2-PA, eIF4G2-PC
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
UniProt
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\eIF4G2 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

2.39

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
spermatogenesis & adult stage | male
spermatocyte
(Franklin-Dumont et al., 2007)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage | male & spermatogenesis
cyst cell of testis
(Ghosh and Lasko, 2015)
male germline cell
(Ghosh and Lasko, 2015)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data

eIF4G2 protein accumulates in the cytoplasm of male germ cells as well as in the surrounding somatic cyst cells in all stages of spermatogenesis.

(Ghosh and Lasko, 2015)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\eIF4G2 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 7 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of eIF4G2
Transgenic constructs containing regulatory region of eIF4G2
Aberrations (Deficiencies and Duplications) ( 6 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (5)
Hsap\EIF4G3
5 of 14
Yes
No
Hsap\EIF4G1
4 of 14
No
No
Hsap\EIF4G2
4 of 14
No
No
Hsap\PAIP1
2 of 14
No
No
1  
Hsap\CTIF
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (6)
Rnor\Eif4g1
5 of 14
Yes
No
Rnor\Eif4g3
5 of 14
Yes
No
Rnor\Eif4g2
4 of 14
No
No
Rnor\Ctif
1 of 14
No
No
Rnor\LOC501116
1 of 14
No
Yes
Rnor\Paip1
1 of 14
No
No
Mus musculus (laboratory mouse) (5)
Mmus\Eif4g1
5 of 14
Yes
No
Mmus\Eif4g3
5 of 14
Yes
No
Mmus\Eif4g2
4 of 14
No
No
Mmus\Paip1
2 of 14
No
No
Mmus\Ctif
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (6)
Xtro\eif4g1
4 of 13
Yes
No
Xtro\eif4g3
4 of 13
Yes
No
Xtro\cptp
1 of 13
No
No
Xtro\ctif
1 of 13
No
No
Xtro\eif4g2
1 of 13
No
No
Xtro\mars1
1 of 13
No
No
Danio rerio (Zebrafish) (8)
Drer\eif4g1a
5 of 14
Yes
No
Drer\eif4g2a
4 of 14
No
No
Drer\eif4g2b
4 of 14
No
No
Drer\eif4g3a
4 of 14
No
No
Drer\eif4g3b
4 of 14
No
No
Drer\paip1
2 of 14
No
No
Drer\wu:fb16f03
2 of 14
No
No
Drer\ctif
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\ifg-1
5 of 14
Yes
No
Anopheles gambiae (African malaria mosquito) (4)
Agam\AgaP_AGAP002502
5 of 12
Yes
No
Agam\AgaP_AGAP004750
3 of 12
No
No
Agam\AgaP_AGAP011627
2 of 12
No
No
Agam\AgaP_AGAP011628
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (5)
Atha\EIF4G
5 of 13
Yes
No
Atha\eIFiso4G1
4 of 13
No
No
Atha\eIFiso4G2
4 of 13
No
No
Atha\AT1G62410
3 of 13
No
Yes
Atha\AT4G30680
3 of 13
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (2)
Scer\TIF4631
5 of 13
Yes
No
Scer\TIF4632
3 of 13
No
No
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\tif471
4 of 12
Yes
No
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:eIF4G2. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (3)
eIF4G1
7 of 13
NAT1
5 of 13
Paip1
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    EIF4G3; eukaryotic translation initiation factor 4 gamma 3
    5 of 14
    603929
        EIF4G1; eukaryotic translation initiation factor 4 gamma 1
        4 of 14
        600495
          EIF4G2; eukaryotic translation initiation factor 4 gamma 2
          4 of 14
          602325
              Functional Complementation Data
              Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
              Interactions
              Summary of Physical Interactions
              esyN Network Diagram
              Show neighbor-neighbor interactions:
              Show/hide secondary interactors 
              (data from AllianceMine provided by esyN)
              Select Layout:
              Legend:
              Protein
              RNA
              Selected Interactor(s)
              Other Interaction Browsers
              View in FlyBase Interactions Browser   View in MIST tool (external link)
              Please see the Physical Interaction reports below for full details
              protein-protein
              Physical Interaction
              Assay
              References
              eIF4G2 - eIF4E1
              anti tag coimmunoprecipitation, anti tag western blot, two hybrid, western blot, anti bait coimmunoprecipitation
              (Ghosh and Lasko, 2015, Hernandez et al., 2012, Baker and Fuller, 2007)
              eIF4G2 - eIF4E3
              two hybrid, anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation
              (Ghosh and Lasko, 2015, Hernandez et al., 2012)
              eIF4G2 - eIF4E5
              two hybrid
              (Shao et al., 2023)
              eIF4G2 - eIF4E7
              two hybrid
              (Hernandez et al., 2012)
              eIF4G2 - pAbp
              anti bait coimmunoprecipitation, western blot
              (Ghosh and Lasko, 2015)
              Summary of Genetic Interactions
              esyN Network Diagram
              Show/hide secondary interactors 
              (data from AllianceMine provided by esyN)
              esyN Network Key:
              Suppression
              Enhancement
              Other Interaction Browsers
              View in FlyBase Interactions Browser   View in MIST tool (external link)
              Please look at the allele data for full details of the genetic interactions
              Starting gene(s)
              Interaction type
              Interacting gene(s)
              Reference
              Starting gene(s)
              Interaction type
              Interacting gene(s)
              Reference
              βTub85D
              enhanceable
              eIF4G2
              (Fuller, 1986)
              External Data
              Linkouts
              BioGRID - A database of protein and genetic interactions.
              DroID - A comprehensive database of gene and protein interactions.
              MIST (protein-protein) - An integrated Molecular Interaction Database
              Pathways
              Genomic Location and Detailed Mapping Data
              Chromosome (arm)
              3R
              Recombination map
              3-81
              Cytogenetic map
              95B9-95C1
              Sequence location
              FlyBase Computed Cytological Location
              Cytogenetic map
              Evidence for location
              95B9-95C1
              Limits computationally determined from genome sequence between P{PZ}l(3)0468404684 and P{PZ}Atg600096
              Experimentally Determined Cytological Location
              Cytogenetic map
              Notes
              References
              Experimentally Determined Recombination Data
              Location

              3-81

              (FlyBase, 2016)

              3-82.1

              (Comeron et al., 2012)

              3-82.9 +/- 1.1

              (Fuller, 1986)

              3-82.9

              Left of (cM)
              (Fuller, 1986)
              Right of (cM)
              (Fuller, 1986)
              Notes
              Stocks and Reagents
              Stocks (7)
              Genomic Clones (28)
              cDNA Clones (142)
               

              Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

              cDNA clones, fully sequenced
              BDGP DGC clones
              Other clones
                Drosophila Genomics Resource Center cDNA clones

                For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

                cDNA Clones, End Sequenced (ESTs)
                BDGP DGC clones
                Other clones
                RNAi and Array Information
                Linkouts
                DRSC - Results frm RNAi screens
                Antibody Information
                Laboratory Generated Antibodies
                 

                monoclonal

                (Na et al., 2016)

                polyclonal

                (Ghosh and Lasko, 2015)
                Commercially Available Antibodies
                 
                Cell Line Information
                Publicly Available Cell Lines
                 
                  Other Stable Cell Lines
                   
                    Other Comments
                    Relationship to Other Genes
                    Source for database merge of

                    Source for merge of: eIF4G2 ms(3)nc32

                    (Baker and Fuller, 2007)
                    Additional comments

                    eIF4G2 and CG33111 share an alternatively spliced 5' UTR, but have distinct open reading frames and 3' UTRs.

                    (Franklin-Dumont et al., 2007)
                    Nomenclature History
                    Source for database identify of

                    Source for identity of: eIF4G2 ofs

                    (Franklin-Dumont et al., 2007)

                    Source for identity of: ofs CG10192

                    (Franklin-Dumont et al., 2007)
                    Nomenclature comments
                    Etymology
                    Synonyms and Secondary IDs (12)
                    Datasets (0)
                    Study focus (0)
                    Experimental Role
                    Project
                    Project Type
                    Title
                    Study result (0)
                    Result
                    Result Type
                    Title
                    External Crossreferences and Linkouts ( 45 )
                    Sequence Crossreferences
                    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                    UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                    Other crossreferences
                    AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                    DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                    EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                    FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                    InterPro - A database of protein families, domains and functional sites
                    KEGG Genes - Molecular building blocks of life in the genomic space.
                    MARRVEL_MODEL - MARRVEL (model organism gene)
                    Linkouts
                    BioGRID - A database of protein and genetic interactions.
                    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                    DroID - A comprehensive database of gene and protein interactions.
                    DRSC - Results frm RNAi screens
                    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                    Flygut - An atlas of the Drosophila adult midgut
                    FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
                    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
                    MIST (protein-protein) - An integrated Molecular Interaction Database
                    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                    References (57)